Evaluation of two different protocols for peripheral blood stem cell collection with the Fresenius AS 104 blood cell separator.

Abstract:

OBJECTIVES:Reconstitution of hematopoiesis by means of peripheral blood stem cells is a valid alternative to autologous bone marrow transplantation. The aim of this investigation was to increase the efficiency of collection of circulating blood progenitor cells and to obtain a purer product for transplant. METHODS:We carried out leukapheresis procedures with the Fresenius AS 104 blood cell separator, using two different protocols, the previously used PBSC-LYM and a new mononuclear cell collection program. RESULTS:Both programs were highly effective in collecting mononuclear cells (MNC) and CD34+ cells. Some differences were found, especially regarding MNC yield and efficiencies. There are remarkable differences in the efficiency of collection of CD34+ cells (62.38% with the new program as opposed to 31.69% with the older one). Linear regression analysis showed a negative correlation between blood volume processed and MNC efficiency only for the PBSC-LYM program. Differences were also observed in the degree of inverse correlation existing in both programs between patients' white blood cell precount and MNC collection efficiency. The inverse correlation was stronger for the PBSC-LYM program. Seven patients with solid tumors and hematologic malignancies received high dose chemotherapy and were subsequently transplanted with peripheral blood stem cells collected using the new protocol. All patients obtained a complete and stable engraftment with the reinfusion product collected with one or two leukapheresis procedures. CONCLUSIONS:High efficiencies and yields were observed in the new protocol for MNC and CD34+ cells. These were able to effect rapid and complete bone marrow recovery after myeloablative chemotherapy.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Menichella G,Lai M,Pierelli L,Vittori M,Serafini R,Ciarli M,Foddai ML,Salerno G,Sica S,Scambia G,Leone G,Bizzi B

doi

10.1046/j.1423-0410.1997.7340230.x

subject

Has Abstract

pub_date

1997-01-01 00:00:00

pages

230-6

issue

4

eissn

0042-9007

issn

1423-0410

journal_volume

73

pub_type

临床试验,杂志文章
  • Variant Creutzfeldt-Jakob disease transmission by plasma products: assessing and communicating risk in an era of scientific uncertainty.

    abstract::A substantial body of animal data indicates that transmissible spongiform encephalopathies (TSEs) are transmitted through blood. These data have been augmented in the past year by reports that two recipients of red cells from donors with variant Creutzfeldt-Jakob disease (vCJD) in the United Kingdom have acquired this...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.2005.00702.x

    authors: Farrugia A,Ironside JW,Giangrande P

    更新日期:2005-11-01 00:00:00

  • Immune tolerance protocols using low-dose factor VIII: a review of the literature.

    abstract::Low-dose immune tolerance protocols use doses of factor VIII of less than 50 IU/kg/day to induce immune tolerance. Such protocols are chosen for a variety of reasons including cost, patient acceptability and ease of administration. Early published reports of low-dose protocols appeared in 1981, described small numbers...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1159/000056718

    authors: Sultan Y

    更新日期:1999-01-01 00:00:00

  • Accumulation of soluble inflammatory mediators between blood donation and pre-storage leucocyte depletion.

    abstract:BACKGROUND AND OBJECTIVES:Leucocyte-derived cytokines accumulate in stored whole blood. Pre-storage leucocyte depletion has reduced cytokine levels and, consequently, febrile non-haemolytic transfusion reactions. As leucocyte filtration and component separation can be performed until 24 h after donation, we hypothesize...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2008.01132.x

    authors: Chudziak D,Sireis W,Pfeiffer HU,Henschler R,Seifried E,Bönig H

    更新日期:2009-02-01 00:00:00

  • Platelets stored in a new-generation container differences between plasma and platelet additive solution II.

    abstract:BACKGROUND AND OBJECTIVES:In order to preserve platelet concentrates (PCs) with high yields, a new polyolefin platelet storage container (PL 2410, 1.3L, Baxter, La Châtre, France) with increased gas permeability in combination with a larger surface area has been developed. The storage capacity was studied with platelet...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:

    authors: de Wildt-Eggen J,Schrijver JG,Smid WM,Joie M,Bollinne V,Bins M

    更新日期:1998-01-01 00:00:00

  • A multicentre evaluation of a new filtration protocol for leucocyte depletion of high-haematocrit red blood cells collected by an automated blood collection system.

    abstract:BACKGROUND AND OBJECTIVES:A multicentre trial was set up to evaluate the performance of a new leucodepletion protocol. MATERIALS AND METHODS:Filtration at high haematocrit was started during collection of red blood cell (RBC) products by apheresis with Trima. SAG-M was added after filtration through the filter. Haemat...

    journal_title:Vox sanguinis

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1046/j.1423-0410.2001.00078.x

    authors: Soli M,Blanco L,Riggert J,Martínez-Clavel A,Lucas C,Lunghi M,Belloni M,Wolf C,van Waeg G,Antoon M

    更新日期:2001-01-01 00:00:00

  • Biochemistry of whole blood in poly(ethylene-co-ethylacrylate) experimental blood containers.

    abstract::The biochemical status of whole blood stored in containers fabricated of ethylene ethylacrylate (EEA) film was monitored at several times during 4 weeks of storage at 4 degrees C. Fifteen biochemical indicators were studied to reflect on erythrocyte integrity, cellular metabolism, plasma protein stability, and microag...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1985.tb00161.x

    authors: Horowitz B,Rowe AW,Stryker MH,Van der Sande J,Waldman AA,Wong SC,Gass JD,Woods KR

    更新日期:1985-01-01 00:00:00

  • Reduced risk of transfusion-transmitted HIV in Kenya through centrally co-ordinated blood centres, stringent donor selection and effective p24 antigen-HIV antibody screening.

    abstract:BACKGROUND:Following a 1994 study showing a high rate of transfusion-associated HIV, Kenya implemented WHO blood safety recommendations including: organizing the Kenya National Blood Transfusion Service (NBTS), stringent blood donor selection, and universal screening with fourth-generation p24 antigen and HIV antibody ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2010.01340.x

    authors: Basavaraju SV,Mwangi J,Nyamongo J,Zeh C,Kimani D,Shiraishi RW,Madoda R,Okonji JA,Sugut W,Ongwae S,Pitman JP,Marum LH

    更新日期:2010-10-01 00:00:00

  • False reactions in radioimmunoassay for viral hepatitis B markers in patients suffering from coagulation disorders.

    abstract::Simultaneous occurrence of hepatitis B surface antigen (HBsAg) and antibodies to HBsAg (anti-HBs) has been frequently described in hemophiliac patients. During our working procedures, this phenomenon was also observed. Interference of coagulation disorders with radioimmunoassay (RIA) used in the detection of viral hep...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1982.tb01088.x

    authors: Grangeot-Keros L,Lambert T,Dubreuil P,Briantais MJ,Pillot J

    更新日期:1982-03-01 00:00:00

  • Brb, a platelet alloantigen involved in neonatal alloimmune thrombocytopenia.

    abstract::Serum from a pregnant woman with the May-Hegglin anomaly contained a platelet-specific antibody. The serum reacted in the platelet indirect immunofluorescence test (PIIFT) with 97.6% of random donor platelets and those of the father but not with the mother's own platelets. This antibody induced a moderate thrombocytop...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1991.tb00911.x

    authors: Bettaieb A,Fromont P,Rodet M,Godeau B,Duedari N,Bierling P

    更新日期:1991-01-01 00:00:00

  • Antenatal blood group serology in Sudanese women.

    abstract::2,140 pregnant Sudanese women attending the antenatal clinic in Omdurman Maternity Hospital during the period November 1975 to December 1976 had ABO and Rh typing performed. Their sera were also screened by a modified papain enzyme technique for the presence of atypical antibodies. The rate of Rhesus immunization, the...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1978.tb02889.x

    authors: Abu-Sin AY,Ayoub M,Abdelrazig H

    更新日期:1978-01-01 00:00:00

  • Transfusion history, T cell subsets and natural killer cytotoxicity in patients with colorectal cancer.

    abstract::Blood transfusions are associated with clinical phenomena which are attributable to immune suppression. Since suppression of immune function is associated with a high risk of spontaneous malignancies, we studied T cell subsets and natural killer (NK) cytotoxicity in colorectal cancer patients and correlated the result...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1989.tb04954.x

    authors: Tartter PI,Steinberg B,Barron DM,Martinelli G

    更新日期:1989-01-01 00:00:00

  • In vitro storage characteristics of platelet concentrates suspended in 70% SSP+(TM) additive solution versus plasma over a 14-day storage period.

    abstract:BACKGROUND AND OBJECTIVES:The non-paired two-arm study compared the in vitro storage characteristics of platelets suspended as concentrates in either 100% plasma or a mixture of additive solution (SSP+™, MacoPharma, Mouveaux, France) and autologous plasma in a 70:30 ratio over a 14-day storage period. MATERIALS AND ME...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2011.01468.x

    authors: Saunders C,Rowe G,Wilkins K,Holme S,Collins P

    更新日期:2011-08-01 00:00:00

  • A Japanese multi-institutional collaborative study of antigen-positive red blood cell (RBC) transfusions in patients with corresponding RBC antibodies.

    abstract:BACKGROUND AND OBJECTIVES:It is sometimes difficult to obtain antigen-negative red blood cells (RBCs) for patients with antibodies against RBCs. However, the frequency and severity of the adverse reactions have not been well elucidated. Here, we conducted a multi-institutional collaborative study to clarify the backgro...

    journal_title:Vox sanguinis

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1111/vox.12906

    authors: Yamada C,Takeshita A,Ohto H,Ishimaru K,Kawabata K,Nomaguchi Y,Haraguchi Y,Abe M,Sobue K,Takenouchi H,Takadate J,Kamimura M,Katai A,Kasai D,Minami Y,Sugimoto T,Michino J,Nagai K,Kumagai M,Hasegawa Y,Ishizuka K,Oh

    更新日期:2020-07-01 00:00:00

  • Photoinactivation of viruses in human fresh plasma by phenothiazine dyes in combination with visible light.

    abstract::We developed a photodynamic method to inactivate viruses in human fresh plasma. Single plasma bags were illuminated with visible light in the presence of low doses of phenothiazine dyes like methylene blue or toluidine blue. By this treatment the infectivity of different enveloped viruses including the causative agent...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1991.tb00907.x

    authors: Lambrecht B,Mohr H,Knüver-Hopf J,Schmitt H

    更新日期:1991-01-01 00:00:00

  • Immunofluorescent staining of platelet suspensions and detection of antiplatelet antibody in patients with idiopathic thrombocytopenic purpura.

    abstract::Direct immunofluorescent staining of 31 specimens of platelets obtained from 13 cases of idiopathic thrombocytopenic purpura (ITP) revealed positive staining on the surface of platelets for both immunoglobulins (Igs) and human B1C globulin in 9 specimens, for only Igs in 1 specimen and for human B1C alone in 5 specime...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1979.tb04410.x

    authors: Boonpucknavig S,Vuttivirojana O,Srichaikul T

    更新日期:1979-01-01 00:00:00

  • Transfusion transmitted hepatitis C and non-A, non-B, non-C.

    abstract::The decline in the incidence of TAH from 1985-1990 was considerable and based on a variety of interventions that led to increasingly intensive scrutiny of potential donors. The decline in incidence observed since 1990, and particularly since 1992, has been truly extraordinary and based principally on the introduction ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.1994.tb04539.x

    authors: Alter HJ

    更新日期:1994-01-01 00:00:00

  • Posttransfusion non-A, non-B hepatitis after cardiac surgery: a prospective study.

    abstract::In a prospective study of 50 recipients of HBsAg-negative blood who had undergone cardiac surgery, 4 (8%) developed acute non-A, non-B hepatitis. The patients who developed hepatitis had received significantly more units of blood or blood products than the patients who had no hepatitis. The incubation period of the di...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1983.tb01919.x

    authors: Tur-Kaspa R,Shimon DV,Shalit M,Adler R,Shraga S,Manny N,Morag A,Eliakim M

    更新日期:1983-01-01 00:00:00

  • Use of the MAIEA technique to confirm the relationship between the Cromer antigens and decay-accelerating factor and to assign provisionally antigens to the short-consensus repeats.

    abstract::The MAIEA (monoclonal-antibody-specific immobilisation of erythrocyte antigens) assay has recently been developed for the assignment of red cell antigens, recognised by human alloantisera, to particular membrane components of the red cell membrane. This technique detects trimolecular complexes formed by the reaction o...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1993.tb02172.x

    authors: Petty AC,Daniels GL,Anstee DJ,Tippett P

    更新日期:1993-01-01 00:00:00

  • Molecular genetic analysis of para-Bombay phenotypes in Chinese: a novel non-functional FUT1 allele is identified.

    abstract:BACKGROUND AND OBJECTIVES:The para-Bombay phenotype (also known as H-deficient secretor) is characterized by a lack of ABH antigens on red cells, but ABH substances are found in saliva. Molecular genetic analysis was performed for five Chinese individuals serologically typed as para-Bombay. MATERIALS AND METHODS:ABO g...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1046/j.1423-0410.2002.00184.x

    authors: Yip SP,Chee KY,Chan PY,Chow EY,Wong HF

    更新日期:2002-10-01 00:00:00

  • Effectiveness of four intravenous IgG preparations in prevention of phagocytosis of anti-Rh-(D)-coated erythrocytes by mononuclear phagocytes.

    abstract::Four commercial intravenous immunoglobulin G (IGIV) preparations, Sandoglobulin (Sandoz), Intraglobin (Biotest), Gammonativ (Kabivitrum) and Gammaogard (Kabivitrum) showed percent inhibition of Fc receptor-mediated phagocytosis of anti-Rh(D)-coated erythrocytes as follows: 45 +/- 14 SD, 41 +/- 23 SD, 35 +/- 14 SD, 25 ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1993.tb02145.x

    authors: Sheth KV,al-Sedairy S,Lee J

    更新日期:1993-01-01 00:00:00

  • Therapeutic efficacy of platelet components treated with amotosalen and ultraviolet A pathogen inactivation method: results of a meta-analysis of randomized controlled trials.

    abstract:BACKGROUND AND OBJECTIVES:There are conflicting data regarding the therapeutic efficacy of platelets inactivated using amotosalen and ultraviolet A light. We have performed a meta-analysis to summarize the results of different randomized controlled trials (RCT). MATERIALS AND METHODS:Five RCTs reported through March 2...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,meta分析

    doi:10.1111/j.1423-0410.2012.01614.x

    authors: Cid J,Escolar G,Lozano M

    更新日期:2012-11-01 00:00:00

  • The influence of platelet glycoprotein polymorphisms on receptor function and risk for thrombosis.

    abstract::With regard to hemostasis and thrombosis, collagens are among the most important physiologic components of the extracellular matrix in their role as platelet activators. Differences in the rate of platelet activation markedly influence normal hemostasis and the pathological outcome of thrombosis. Thus, collagen recept...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.2002.tb05274.x

    authors: Kunicki TJ,Nugent DJ

    更新日期:2002-08-01 00:00:00

  • Non-HLA lymphocytotoxic antibodies in HIV-seropositive and HIV-seronegative hemophiliacs.

    abstract::We tested serum samples from 11 HIV-seropositive and 32 HIV-seronegative Italian patients with hemophilia A or B for the presence of lymphocytotoxic antibodies (LCTAs). No patients had the acquired immune deficiency syndrome and all had received over many years heat-untreated commercial clotting factors. LCTAs directe...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1988.tb01612.x

    authors: Conte R,Giovanardi L,Tazzari PL,De Rosa V,Rodorigo G,Re MC

    更新日期:1988-01-01 00:00:00

  • Blood donations indeterminate in HIV-1 western blot analysed by IgM immunoblot and polymerase chain reaction.

    abstract::The presence of IgM antibodies to human immunodeficiency virus 1 (HIV-1) was investigated in blood donor sera which were indeterminate in anti-HIV-1 IgG Western blot testing. In 7 of 173 instances out of approximately 1,000,000 blood donation sera with an isolated anti-p24 IgG produced an anti-gp41-45 IgM immunoblot r...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1993.tb02524.x

    authors: Knüver-Hopf J,Heinze H,Lambrecht B,Mohr H,Beyer J,Schmitt H

    更新日期:1993-01-01 00:00:00

  • Organizational models for health actions in the field of blood and blood products in Brazilian and Spanish regional contexts.

    abstract:BACKGROUND AND OBJECTIVES:This article aims to characterize some elements that define the organizational models of healthcare actions in the field of blood and blood products. MATERIALS AND METHODS:This exploratory and qualitative study was carried out based on a documentary survey complemented by interviews with key ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12665

    authors: Souza MKB

    更新日期:2018-05-21 00:00:00

  • External quality assessment for the detection of HCV RNA, HIV RNA and HBV DNA in plasma by nucleic acid amplification technology: a novel approach.

    abstract:BACKGROUND AND OBJECTIVES:In this EQA study a novel approach was used to assess the performance of blood centres and blood product manufacturers in detecting the possible contamination of plasma with HCV, HIV and HBV by NAT. MATERIALS AND METHODS:A panel of 12 samples, three negative and three positive for each virus,...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2008.01047.x

    authors: Pisani G,Marino F,Cristiano K,Bisso GM,Mele C,Luciani F,Wirz M,Gentili G,External Quality Assessment Participants.

    更新日期:2008-07-01 00:00:00

  • Transfusion practice in massively bleeding patients: time for a change?

    abstract:BACKGROUND AND OBJECTIVES:We identified some fatal cases where massively bleeding patients received inadequate transfusion therapy. The aim of this study was to review and evaluate the transfusion practice in acutely multitransfused patients. MATERIALS AND METHODS:Patients receiving > 10 units of red blood cells (RBC)...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2005.00668.x

    authors: Johansson PI,Hansen MB,Sørensen H

    更新日期:2005-08-01 00:00:00

  • Detection of early anti-p24 HIV responses in EIA- and immunoblot-negative individuals. Implications for confirmatory testing.

    abstract::A sensitive and specific radioimmunoprecipitation assay was developed for the detection and analysis of anti-HIV antibody response in human sera with the use of 125I-labelled purified HIV proteins with subsequent sodium-dodecylsulfate gel electrophoresis (125I-RIPA). The 125I-RIPA was shown to be as specific but at le...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1987.tb04910.x

    authors: Huisman JG,Winkel IN,Lelie PN,Tersmette M,Goudsmit J,Miedema F

    更新日期:1987-01-01 00:00:00

  • Hepatitis E in transfusion-dependent thalassaemia patients, in Greece: a single centre experience.

    abstract::Hepatitis E is considered an emerging disease that may be a threat in both developing and industrialized countries all over the world. The risk of chronic hepatitis E virus infection is higher among immunocompromised patients. This study aimed to assess the status of hepatitis E infection in patients with transfusion-...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12572

    authors: Klonizakis P,Gioula G,Exindari M,Apostolou C,Kotsiafti A,Vlachaki E

    更新日期:2017-10-01 00:00:00

  • Impact of tobacco smoking on platelet function in apheresis products in vitro.

    abstract:BACKGROUND AND OBJECTIVES:The aim of this study was to analyse the platelet function, over a 5-day time-period, of apheresis-derived platelet concentrates obtained from smokers and non-smokers. MATERIALS AND METHODS:Smoker and non-smoker plateletpheresis products were investigated on days 1, 3 and 5 of storage. Recept...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.0042-9007.2004.00507.x

    authors: Scheinichen D,Heuft HG,Renken C,Jüttner B,Jaeger K,Schürholz T,Blasczyk R,Piepenbrock S,Elsner HA

    更新日期:2004-05-01 00:00:00