Use of the MAIEA technique to confirm the relationship between the Cromer antigens and decay-accelerating factor and to assign provisionally antigens to the short-consensus repeats.

Abstract:

:The MAIEA (monoclonal-antibody-specific immobilisation of erythrocyte antigens) assay has recently been developed for the assignment of red cell antigens, recognised by human alloantisera, to particular membrane components of the red cell membrane. This technique detects trimolecular complexes formed by the reaction of a human antibody and a mouse antibody with a particular red cell protein. A positive reaction, in an ELISA-type detection procedure, occurs if the epitopes to the human and mouse antibodies are present on the same membrane component but at different regions. In this report, we show how the MAIEA assay can be used to confirm the relationship between Cromer system antigens and the complement-regulatory protein, decay-accelerating factor (DAF, CD 55). In addition, the location of the antigens along the protein is postulated by using three anti-DAF monoclonal antibodies with specificities to different regions of DAF. Tca and Esa are assigned provisionally to the first short-consensus repeat (SCR), UMC to the second SCR, Dra to the third SCR and Cra, WESa and WESb to the fourth SCR or to the serine/threonine rich region of the DAF protein.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Petty AC,Daniels GL,Anstee DJ,Tippett P

doi

10.1111/j.1423-0410.1993.tb02172.x

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

309-15

issue

4

eissn

0042-9007

issn

1423-0410

journal_volume

65

pub_type

杂志文章
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    doi:10.1111/j.1423-0410.1979.tb04426.x

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    doi:

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    pub_type: 杂志文章

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