Prolonged storage of red cells: the effect of pH, adenine and phosphate.

Abstract:

:Prolonged storage of red blood cells (RBCs) at 4 degrees C results in decreased intracellular ATP levels with diminished posttransfusion survival. Meryman described a preservative medium, exceptional in its capacity to increase these intracellular levels during the first weeks of storage and later in maintaining adequate levels, for extended storage periods. We modified this medium, investigated its constituents, and found that its ATP-preserving effect was unrelated to its tonicity or to the presence of mannitol. Throughout storage, RBC potassium leakage and lactate production were moderate. No evidence of osmotic swelling was detected. In spite of high ATP levels, the cells became echinocytes, thus discounting a direct correlation between shape and metabolic status. The most striking finding in this study was that the prestorage pH of the blood unit (pH 7.0), has a crucial contribution in elevating nucleotide levels in a medium containing high levels of phosphate (18-40 mM) and adenine. We suggest that a combined effect of optimal pH, adenine, glucose and phosphate in the medium contributes to the ability of the RBCs to synthesize the necessary purine nucleotides by the 'salvage pathway'.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Mazor D,Dvilansky A,Meyerstein N

doi

10.1111/j.1423-0410.1994.tb00327.x

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

264-9

issue

4

eissn

0042-9007

issn

1423-0410

journal_volume

66

pub_type

杂志文章
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    pub_type: 杂志文章

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  • Inactivation of severe acute respiratory syndrome coronavirus 2 in plasma and platelet products using a riboflavin and ultraviolet light-based photochemical treatment.

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  • Iron deficiency among French whole-blood donors: first assessment and identification of predictive factors.

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  • Improvement of enzyme-linked antiglobulin test by using an antiglobulin linked to glucose oxidase: description of the technique.

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