Application of the gel test in IgG subclassing--a comparison of two agglutination assays.

Abstract:

BACKGROUND AND OBJECTIVES:IgG subclasses of anti-D seem to play some role in hemolytic disease of the newborn, but there has been disagreement as to its exact nature. The aim of our study was to evaluate a new technique for IgG subclassing of anti-D and to compare it to an established test. MATERIALS AND METHODS:In 31 cases of RhD immunization, we have compared two simple agglutinating assays for subclassing anti-D: an established V-well microtiter tray assay and a new gel test assay. Polyclonal rabbit anti-IgG agglutinating antibodies were tested against sensitized D-positive red blood cells by both assays. RESULTS:The anti-D concentration in tested sera varied between 0.1 and 81 micrograms/ml (0.5-405 IU/ml). Both assays were simple to perform and the results correlated well. CONCLUSIONS:The gel test had two advantages: quick performance and easy interpretation.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Palfi M,Hildén JO

doi

10.1046/j.1423-0410.1997.7220114.x

subject

Has Abstract

pub_date

1997-01-01 00:00:00

pages

114-7

issue

2

eissn

0042-9007

issn

1423-0410

journal_volume

72

pub_type

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