Abstract:
BACKGROUND AND OBJECTIVES:IgG subclasses of anti-D seem to play some role in hemolytic disease of the newborn, but there has been disagreement as to its exact nature. The aim of our study was to evaluate a new technique for IgG subclassing of anti-D and to compare it to an established test. MATERIALS AND METHODS:In 31 cases of RhD immunization, we have compared two simple agglutinating assays for subclassing anti-D: an established V-well microtiter tray assay and a new gel test assay. Polyclonal rabbit anti-IgG agglutinating antibodies were tested against sensitized D-positive red blood cells by both assays. RESULTS:The anti-D concentration in tested sera varied between 0.1 and 81 micrograms/ml (0.5-405 IU/ml). Both assays were simple to perform and the results correlated well. CONCLUSIONS:The gel test had two advantages: quick performance and easy interpretation.
journal_name
Vox Sangjournal_title
Vox sanguinisauthors
Palfi M,Hildén JOdoi
10.1046/j.1423-0410.1997.7220114.xsubject
Has Abstractpub_date
1997-01-01 00:00:00pages
114-7issue
2eissn
0042-9007issn
1423-0410journal_volume
72pub_type
杂志文章相关文献
VOX SANGUINIS文献大全abstract:BACKGROUND AND OBJECTIVES:This study was undertaken to provide denominator data relating to the timing and location of transfusion, to support interpretation of reports of incorrect blood component transfused (IBCT) events to the UK Serious Hazards of Transfusion (SHOT) scheme. MATERIALS AND METHODS:The study was carr...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2007.00952.x
更新日期:2007-10-01 00:00:00
abstract::Hepatitis E virus (HEV)-positive plasma donations, identified by a plasma mini-pool screening approach, were analysed using serological methods for the presence of anti-HEV IgM and IgG. Avidity testing was performed on the IgG-reactive donations. Anti-HEV IgG with high avidity was observed in two donors together with ...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.12294
更新日期:2015-11-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Leucocyte-derived cytokines accumulate in stored whole blood. Pre-storage leucocyte depletion has reduced cytokine levels and, consequently, febrile non-haemolytic transfusion reactions. As leucocyte filtration and component separation can be performed until 24 h after donation, we hypothesize...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2008.01132.x
更新日期:2009-02-01 00:00:00
abstract::Four Kp(a-b-) Japanese sisters, the product of a consanguineous mating, have otherwise normal Kell antigens. Kpc, a 'new' allele of Kpa and Kpb is the most probable background. ...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1979.tb04395.x
更新日期:1979-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The results of quality monitoring leucocyte counts were analysed nationally for the first 2 years of universal leucocyte depletion (LD), spanning the time-period before and after standardization of the counting and LD methods. The objectives were twofold: first to determine whether the impleme...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1046/j.1423-0410.2002.00210.x
更新日期:2002-08-01 00:00:00
abstract::The interaction of temperature and pH in biological systems comprises two components. Temperature change may perturb the pH of solutions, and it may change the pKa of some ionizable groups that are involved in enzyme catalysis. The pH optima of single reactions and whole pathways are therefore temperature sensitive. T...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1992.tb01173.x
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Serratia marcescens is a gram-negative bacterium that has been implicated in adverse transfusion reactions associated with contaminated platelet concentrates. The aim of this study was to investigate whether the ability of S. marcescens to form surface-attached aggregates (biofilms) could acco...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2011.01550.x
更新日期:2012-04-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:B subgroups are rare and the genetic analysis reported to date has been limited. MATERIALS AND METHODS:Serological and molecular investigations were performed in blood from a B-subgroup donor. RESULTS:Red cells did not react with anti-B and anti-AB reagents. However, cells absorbed anti-B. R...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2005.00583.x
更新日期:2005-01-01 00:00:00
abstract::Since 2000, Quality Assurance (QA) exercises for the detection and identification of granulocyte antibodies and DNA typing for human neutrophil antigens (HNA) have been distributed within the International Granulocyte Immunobiology Workshops, which are linked to International Society of Blood Transfusion. The exercise...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.12041
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:This article aims to characterize some elements that define the organizational models of healthcare actions in the field of blood and blood products. MATERIALS AND METHODS:This exploratory and qualitative study was carried out based on a documentary survey complemented by interviews with key ...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.12665
更新日期:2018-05-21 00:00:00
abstract::An alternative to blood transfusion, based on oxygen-carrying solutions, has been sought for over a century. The present 'first-generation' haemoglobin-products were based on observations that crosslinking with, for example, glutaraldehyde, overcame subunit dissociation and renal toxicity. Experience with these soluti...
journal_title:Vox sanguinis
pub_type: 杂志文章,评审
doi:10.1111/j.1423-0410.2006.00789.x
更新日期:2006-08-01 00:00:00
abstract:OBJECTIVES:Positive direct antiglobulin tests (DAT) occur occasionally, without obvious cause, among normal blood donors. This is sometimes associated with a false-positive serologic test for syphilis. Positive DATs due to antiphospholipid antibodies (APAbs) are well documented in the antiphospholipid syndrome with sys...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1046/j.1423-0410.1997.7230182.x
更新日期:1997-01-01 00:00:00
abstract::A new high-frequency antigen related to the Kell system was defined by the serum of a patient, Mrs. Sub. The antigen was lacking from her cells and those of one of her three siblings. All random cells tested were incompatible with her serum as were bloods of other various "null" phenotypes, but Ko bloods were compatib...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1979.tb04406.x
更新日期:1979-01-01 00:00:00
abstract::Extracts of seeds from 79 plant species were tested for agglutinating and lytic activity with the red cells of ten vertebrate species (man, monkey, rabbit, rat, goat, sheep, guinea pig, horse, fowl, dog). Of these extracts, 24 were hemagglutinating and 15 were hemolytic. The results suggest that some lectins can serve...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1978.tb02928.x
更新日期:1978-01-01 00:00:00
abstract:BACKGROUND:The rationale for using topical platelet gel therapy is to provide the healing tissues with concentrated platelet-derived factors. Several systems are available to prepare platelet-rich plasma (PRP) and from these, the platelet gel. These systems produce two- to six-fold platelet and growth factor-enriched c...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2009.01188.x
更新日期:2009-08-01 00:00:00
abstract::Alpha1-Acid glycoprotein (orosomucoid) was prepared from a byproduct of the ethanol plasma fractionation by means of ion-exchange procedures. Immunoelectrophoresis suggested a high degree of purity; the purified protein contained 13.5% sialic acid and 17.8% hexose. The alpha1-acid glycoprotein was modified by removal ...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1976.tb02846.x
更新日期:1976-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Apheresis platelet concentrates sometimes contain persistent aggregates (PA). Because apheresis involves extracorporeal circulation, we hypothesized that interactions between GPIbα and von Willebrand factor (VWF) underlie their origin. MATERIALS AND METHODS:Platelets in donations with PA were...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.12365
更新日期:2016-05-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Capillary samples can provide blood for cell counts in haematologic patients and blood donors. However, some accept only values from venous blood. This study compares capillary and venous blood counts to verify the hypothesis that they are equivalent. MATERIALS AND METHODS:We analysed 463 cap...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2007.00978.x
更新日期:2007-11-01 00:00:00
abstract::Activated protein C (APC) is a highly specific serine proteinase which functions as an important naturally occurring antithrombotic enzyme. APC also has anti-inflammatory properties. We have developed a large-scale process for the production of APC for therapeutic use starting with cryoprecipitate-poor human plasma. T...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1995.tb00366.x
更新日期:1995-01-01 00:00:00
abstract::Blood transfusion is used as a life-saving and prophylactic treatment in sickle cell disease. Despite the many complications associated with its use, few randomised controlled trials and careful research studies have been performed to fully define its role. This subjects is, therefore, discussed in the context of the ...
journal_title:Vox sanguinis
pub_type: 杂志文章,评审
doi:10.1111/j.1423-0410.1995.tb03916.x
更新日期:1995-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Gene conversion events between GYPA and GYPB or GYPA and GYPE are facilitated by the close chromosomal proximity and high degree of sequence homology and can lead to the formation of GP hybrid genes. Discrepant results between blood group genotyping and haemagglutination in 22 random blood don...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.12168
更新日期:2014-11-01 00:00:00
abstract::Neonatal alloimmune thrombocytopenia (NAIT) is usually induced by platelet-specific antibodies against HPA-1a (Zwa) or HPA-5b (Bra). Recently, low-frequency alloantigens on the platelet glycoprotein (GP) IIb/IIIa complex have been discovered as a cause for NAIT. In this report, a new low-frequency platelet-specific al...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1995.tb02604.x
更新日期:1995-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Allergic transfusion reactions (ATRs) and febrile non-haemolytic transfusion reactions (FNHTRs) are the two major types of transfusion-related adverse reactions (TRARs). Although prestorage leucocyte reduction and diversion of the first aliquot of blood (LR/D) could reduce FNHTRs and bacterial...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.12373
更新日期:2016-05-01 00:00:00
abstract::beta 2-Microglobulin is a low molecular weight protein with sequence homology to immunoglobulins. As a portion of the HLA complex this protein is an important cell-surface structure. Under normal conditions beta 2-microglobulin is synthesized and shed by many cells, particularly lymphocytes, and is detectable in the c...
journal_title:Vox sanguinis
pub_type: 杂志文章,评审
doi:10.1111/j.1423-0410.1980.tb04500.x
更新日期:1980-06-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Several pathogen inactivation methods currently applied to therapeutic plasma may result in products with different hemostatic properties. This study aims at evaluating and comparing the hemostatic potential of different therapeutic plasma preparations currently available in France. MATERIALS...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2011.01562.x
更新日期:2012-05-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:There are conflicting data regarding the therapeutic efficacy of platelets inactivated using amotosalen and ultraviolet A light. We have performed a meta-analysis to summarize the results of different randomized controlled trials (RCT). MATERIALS AND METHODS:Five RCTs reported through March 2...
journal_title:Vox sanguinis
pub_type: 杂志文章,meta分析
doi:10.1111/j.1423-0410.2012.01614.x
更新日期:2012-11-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Platelet concentrates are frequently transfused to patients with reduced immunity. An exhaustive description of their viral content is needed to prevent unwanted infection. MATERIAL AND METHODS:To track viral sequences, a shotgun metagenomics approach was used on a bank of 300 platelets conce...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.12695
更新日期:2018-07-18 00:00:00
abstract::Monocyte-erythrocyte interaction in patients with autoimmune haemolytic anaemia (AIHA) was assessed by phagocytosis and rosette assays. In most patients, a relationship was observed between haemolysis and the phagocytosis of their own erythrocytes by allogenic peripheral monocytes. An evaluation of the number of immun...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1987.tb03030.x
更新日期:1987-01-01 00:00:00
abstract::The successful cloning of a non-structural antigen from the genome of what is now designated as the 'hepatitis C virus' (HCV) has transformed an erstwhile diagnosis of exclusion for non-A, non-B hepatitis (NANBH). The assay has been validated against panels of known infectivity for NANBH and sera from haemophiliac pat...
journal_title:Vox sanguinis
pub_type: 杂志文章,评审
doi:10.1111/j.1423-0410.1991.tb00863.x
更新日期:1991-01-01 00:00:00
abstract::Idiopathic analbuminemia was fortuitously detected in a 30-year-old Italian woman. No associated disease condition could be revealed in this patient, the only consistent finding being a slight tendency to develop ankle edema. Asthenia and a moderate hypotension were also recorded. Trace amounts (5.2 mg/100 ml) of seru...
journal_title:Vox sanguinis
pub_type: 杂志文章,评审
doi:10.1111/j.1423-0410.1980.tb01851.x
更新日期:1980-09-01 00:00:00