Mouse monoclonal anti-HBs and its use in the screening of donated blood by Elisa.

Abstract:

:A method is described for the production of mouse monoclonal antibodies to HBsAg. 4 clones produced antibodies directed at the a and one at the d determinant of HBsAg. These antibodies were conjugated to horse radish peroxidase and used in an Elisa for the detection of HBsAg in donated blood. Antibody C10 produced conjugate which rendered the Elisa as sensitive as a commercially obtainable immunoradiometric assay with which it was compared. Conditions for this assay were optimised and it may be used as a rapid (1 h) test for detecting HBsAg-positive blood. It is practicable to read this Elisa by eye instead of photometrically and it can be thus used under field conditions or in emergencies.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Els MC,Govender M,Marimuthu T,Bubb MO,Conradie JD

doi

10.1111/j.1423-0410.1984.tb00070.x

subject

Has Abstract

pub_date

1984-01-01 00:00:00

pages

165-74

issue

3

eissn

0042-9007

issn

1423-0410

journal_volume

46

pub_type

杂志文章
  • Current status of oxygen carriers ('blood substitutes'): 2006.

    abstract::An alternative to blood transfusion, based on oxygen-carrying solutions, has been sought for over a century. The present 'first-generation' haemoglobin-products were based on observations that crosslinking with, for example, glutaraldehyde, overcame subunit dissociation and renal toxicity. Experience with these soluti...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.2006.00789.x

    authors: Winslow RM

    更新日期:2006-08-01 00:00:00

  • Biochemical studies on McLeod phenotype erythrocytes.

    abstract::Red cells of the McLeod phenotype in the Kell blood group system have an acanthocytic morphology. The membrane protein composition analyzed on sodium dodecyl-sulfate-polyacrylamide gel electrophoresis, the ATP level and the activities of a large number of intracellular enzymes appear to be normal. Membranes prepared f...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1981.tb00664.x

    authors: Tang LL,Redman CM,Williams D,Marsh WL

    更新日期:1981-01-01 00:00:00

  • Epitope specificity and inactivation mechanisms of factor VIII inhibitor antibodies.

    abstract::The domain specificity of anti-factor VIII (FVIII) inhibitor antibodies was determined in assays using FVIII domains generated by thrombin cleavage or expressed as recombinant polypeptides to neutralise the inhibitor. The results revealed the existence of three major types of inhibitors, and various combinations of th...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1159/000056708

    authors: Scandella D

    更新日期:1999-01-01 00:00:00

  • BS II lectin: a second hemagglutinin isolated from Bandeiraea simplicifolia seeds with affinity for type III polyagglutinable red cells.

    abstract::BS II lectin, a second hemagglutinin isolated from Bandeiraea simplicifolia seeds, agglutinated the red cells from 11 patients manifesting the 'acquired B' antigen phenomenon. The results of serological studies indicate that this lectin has specificity for the type III polyagglutinable receptors rather than for the 'B...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1977.tb04469.x

    authors: Judd WJ,Beck ML,Hicklin BL,Iyer PN,Goldstein IJ

    更新日期:1977-10-01 00:00:00

  • Increase in glycocalicin levels in platelet concentrates stored in plasma or synthetic medium for 8 days: comparison with other platelet activation markers.

    abstract:BACKGROUND AND OBJECTIVES:Glycocalicin (GC) is a proteolytic fragment of GpIb and can conveniently be measured in supernatants of platelet concentrates (PCs) by means of a sandwich ELISA. Because of the convenience of the assay and easy sample storage, we tested its suitability as a sensitive platelet activation parame...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1159/000031201

    authors: Kostelijk EH,Folman CC,Gouwerok CW,Kramer CM,Verhoeven AJ,de Korte D

    更新日期:2000-01-01 00:00:00

  • A novel second-generation polyolefin container for storage of single-donor apheresis platelets.

    abstract::A non-blow-molded LE-2 polyolefin (PO) container was developed to store single-donor apheresis platelet concentrates (PCs) processed by the Haemonetics Plasma Collecting System (PCS) at 22 degrees C for over 1 day. We molded PO containers with heat-sealing of light-weight polymer alloy films with a thickness of 0.25 m...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1989.tb02022.x

    authors: Shimizu T,Kouketsu K,Kamiya T,Futagawa H,Hirose S

    更新日期:1989-01-01 00:00:00

  • Acute hypoproteinemic fluid overload: its determinants, distribution, and treatment with concentrated albumin and diuretics.

    abstract::We simulated the use of massive volumes of crystalloid fluids as a treatment of acute plasma loss in a standardized experimental model and studied the factors determining the retention or excretion of the resulting acute hypoproteinemic fluid overload, its distribution within the body, and its treatment with concentra...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1977.tb04481.x

    authors: Pappova E,Bachmeier W,Crevoisier JL,Kollar J,Kollar M,Tobler P,Zahler HW,Zaugg D,Lundsgaard-Hansen P

    更新日期:1977-01-01 00:00:00

  • Mechanism evaluation for an amino acid substitution p.Y246C of B-glycosyltransferase enzyme with Bweak phenotype.

    abstract:BACKGROUND:The amino acid substitutions caused by ABO gene variants are usually predicted to impact the glycosyltransferase function. Here, the effect of an amino acid substitution in the vicinity of the catalytic active region of the B-glycosyltransferase was explored in vitro and in silico study, which is important f...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.13041

    authors: Ying Y,Hong X,Xu X,Zhang J,He J,Zhu F,Xie X

    更新日期:2020-12-16 00:00:00

  • Evaluation of a new microporous filtration membrane system for therapeutic plasma exchange.

    abstract::A new therapeutic plasma exchange device developed by Sarns Inc./3M was evaluated in plasmapheresis of 20 healthy volunteers and in a multicenter clinical study of therapeutic plasma exchange that included 49 patients. Safety and efficacy of plasma separation from whole blood were assessed for a module that contains D...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1987.tb04925.x

    authors: Kurtz SR,Carey PM,McGill M,Pineda AA,Zaroulis CG,Case MT

    更新日期:1987-01-01 00:00:00

  • Serum IgE in polytransfused thalassemic patients.

    abstract::Serum IgE was determined by radioimmunoassay in 28 subjects affected by homozygous beta thalassemia (14 males and 14 females, aged 3-19 years, mean age 7 +/- 5.6 years). They were given transfusions with packed red cells and chelant therapy. Blood samples were taken just before a transfusion (basal value) and at 10-da...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1983.tb01931.x

    authors: La Rosa M,Pavone L,Musumeci S,Mollica F,Ronchetti R

    更新日期:1983-01-01 00:00:00

  • A method for estimating the residual risk of transfusion-transmitted HBV infection associated with occult hepatitis B virus infection in a donor population without universal anti-HBc screening.

    abstract:BACKGROUND AND OBJECTIVES:This report describes a method for estimating the risk of transfusion-transmitted HBV infection attributable to blood components from donors with occult hepatitis B virus infection (OBI) applicable where universal anti-HBc screening is not performed. MATERIALS AND METHODS:In the context of pa...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12060

    authors: Seed CR,Kiely P

    更新日期:2013-11-01 00:00:00

  • Complement and biocompatibility.

    abstract::Blood contact with foreign surfaces induces a certain degree of activation of the defence systems including complement. Recent data using complement specific inhibitors have shown that complement is not only associated with, but in fact contributes to the whole body inflammatory reaction seen as a complication of card...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.1998.tb05435.x

    authors: Mollnes TE

    更新日期:1998-01-01 00:00:00

  • Management of fetal alloimmune thrombocytopenia by weekly in utero platelet transfusions.

    abstract::Alloimmune neonatal thrombocytopenia (ANT) may cause intracranial haemorrhage in utero as well as at delivery. Recent management has concentrated on attempts to minimise fetal thrombocytopenia and prevent its complications. This report describes further experience with the use of repeated intravascular transfusions of...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1990.tb02054.x

    authors: Murphy MF,Pullon HW,Metcalfe P,Chapman JF,Jenkins E,Waters AH,Nicolaides KH,Mibashan RS

    更新日期:1990-01-01 00:00:00

  • "VA", a new type of erythrocyte polyagglutination characterized by depressed H receptors and associated with hemolytic anemia. I. Serological and hematological observations.

    abstract::This report describes a case of persistent polyagglutinability restriced to the red blood cells, and associated with hemolytic anemia in a 20-year-old male. AIME WITH NORMAL INTERVALS. His red blood cells were weakly agglutinated by almost all adult sera. No autoagglutination was noted and the direct antihuman globuli...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:

    authors: Graninger W,Rameis H,Fischer K,Poschmann A,Bird GW,Wingham J,Neumann E

    更新日期:1977-01-01 00:00:00

  • Children with AIDS.

    abstract::The etiologic agent for acquired immune deficiency syndrome (AIDS) has been identified as human T lymphotropic virus type III (HTLV-III). Its distinguishing characteristics and putative receptor, the T4 molecule, are discussed. The Western Blot method has been applied to assess the presence of antibody to HTLV-III in ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1986.tb02005.x

    authors: Pahwa S

    更新日期:1986-01-01 00:00:00

  • Second-generation anti-HCV screening in a Saudi Arabian donor population.

    abstract::The 2nd-generation anti-HCV test system was applied to a Saudi Arabian multi-ethnic donor population. When donors were stratified according to first-time donations versus repeat donations, the latter having been screened previously by a 1st-generation set of tests, it was found that in Saudi Arabian and Middle East na...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1994.tb00273.x

    authors: Bernvil SS,Andrews VJ,Sasich F

    更新日期:1994-01-01 00:00:00

  • Oversight and monitoring of blood safety in the United States.

    abstract::The US blood safety vigilance system is composed of a network of interwoven programs, now organized under a formal structure, with the Assistant Secretary of Health and DHHS Blood Safety Committee bearing overall responsibility. It takes advantage of the breadth of expertise and close collaborative relationship of tra...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1159/000031079

    authors: Busch M,Chamberland M,Epstein J,Kleinman S,Khabbaz R,Nemo G

    更新日期:1999-01-01 00:00:00

  • Competition between ABO and Le gene specified enzymes. I. A Lewis related difference in the amount of A antigen in saliva of A1 and A2 secretors.

    abstract::Radioimmunoassays were prepared using two anti-A and one anti-B reagents. The specificity of the procedures was assessed with 13 artificial antigens. The amounts of A and B natural antigens in saliva of ABH secretors of known Lewis phenotype were measured with these assays. The results confirmed that the average amoun...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:

    authors: Le Pendu J,Lemieux RU,Dalix AM,Lambert F,Oriol R

    更新日期:1983-01-01 00:00:00

  • Cord blood banking and transplant in Europe. Eurocord.

    abstract::The number of cord blood transplants has been increasing very quickly with more than 250 cases reported to Eurocord Registry and more than 500 patients transplanted via the New York Cord Blood Bank. Cord blood transplants have been performed either with related or unrelated cord blood. Several cord blood banks establi...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.1998.tb05403.x

    authors: Gluckman E,Rocha V,Chastang C

    更新日期:1998-01-01 00:00:00

  • A chromatographic procedure for the purification of human plasma albumin.

    abstract::Albumin is obtainable from human blood plasma by an ion exchange chromatographic procedure in a yield of about 95% and a purity well above Pharmacopoeia requirements. Cryosupernatant, factor IX depleted plasma is precipitated with 12 and 25% w/v polyethylene glycol 4000. The second precipitate is dissolved to 8% w/v p...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1977.tb02240.x

    authors: Curling JM,Berglöf J,Lindquist LO,Eriksson S

    更新日期:1977-08-01 00:00:00

  • Improvement of enzyme-linked antiglobulin test by using an antiglobulin linked to glucose oxidase: description of the technique.

    abstract::The classic enzyme-linked antiglobulin test (ELAT) used to detect and quantify the amount of IgG antibodies on red blood cells (RBC) is sensitive to hemolysis and erythrocyte enzymatic activities. We describe a new ELAT by using glucose oxidase (GO) linked to antihuman IgG. The optical density base line of GO-ELAT, al...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1984.tb00098.x

    authors: Rigal D,Monestier M,Lafont S,Raffin T,Got A,Meyer F,Jouvenceaux A

    更新日期:1984-01-01 00:00:00

  • Evaluation of two different protocols for peripheral blood stem cell collection with the Fresenius AS 104 blood cell separator.

    abstract:OBJECTIVES:Reconstitution of hematopoiesis by means of peripheral blood stem cells is a valid alternative to autologous bone marrow transplantation. The aim of this investigation was to increase the efficiency of collection of circulating blood progenitor cells and to obtain a purer product for transplant. METHODS:We ...

    journal_title:Vox sanguinis

    pub_type: 临床试验,杂志文章

    doi:10.1046/j.1423-0410.1997.7340230.x

    authors: Menichella G,Lai M,Pierelli L,Vittori M,Serafini R,Ciarli M,Foddai ML,Salerno G,Sica S,Scambia G,Leone G,Bizzi B

    更新日期:1997-01-01 00:00:00

  • Formation of mono(ethylhexyl)phthalate from di(ethylhexyl)phthalate in human plasma stored in PVC bags and its presence in fractionated plasma proteins.

    abstract::The formation of mono(ethylhexyl)phthalate (MEHP) from di(ethylhexyl)phthalate in human plasma stored in bags of polyvinylchloride has been studied. Substantial amounts were formed and in ten bags from 4 to 56microgram/ml were found. After 2 weeks at room temperature the concentration of MEHP had increased to values b...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1978.tb02903.x

    authors: Vessman J,Rietz G

    更新日期:1978-07-01 00:00:00

  • Predicting massive blood transfusion using clinical scores post-trauma.

    abstract:BACKGROUND AND OBJECTIVES:Early prediction of massive transfusion (MT) post-trauma may reduce mortality by earlier delivery of blood products. A clinical prediction tool (PWH score) for this purpose was developed at the Prince of Wales Hospital, Hong Kong. The aims of this study were to apply this tool to major trauma ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2011.01564.x

    authors: Mitra B,Rainer TH,Cameron PA

    更新日期:2012-05-01 00:00:00

  • Variant Creutzfeldt-Jakob disease transmission by plasma products: assessing and communicating risk in an era of scientific uncertainty.

    abstract::A substantial body of animal data indicates that transmissible spongiform encephalopathies (TSEs) are transmitted through blood. These data have been augmented in the past year by reports that two recipients of red cells from donors with variant Creutzfeldt-Jakob disease (vCJD) in the United Kingdom have acquired this...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.2005.00702.x

    authors: Farrugia A,Ironside JW,Giangrande P

    更新日期:2005-11-01 00:00:00

  • A Japanese multi-institutional collaborative study of antigen-positive red blood cell (RBC) transfusions in patients with corresponding RBC antibodies.

    abstract:BACKGROUND AND OBJECTIVES:It is sometimes difficult to obtain antigen-negative red blood cells (RBCs) for patients with antibodies against RBCs. However, the frequency and severity of the adverse reactions have not been well elucidated. Here, we conducted a multi-institutional collaborative study to clarify the backgro...

    journal_title:Vox sanguinis

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1111/vox.12906

    authors: Yamada C,Takeshita A,Ohto H,Ishimaru K,Kawabata K,Nomaguchi Y,Haraguchi Y,Abe M,Sobue K,Takenouchi H,Takadate J,Kamimura M,Katai A,Kasai D,Minami Y,Sugimoto T,Michino J,Nagai K,Kumagai M,Hasegawa Y,Ishizuka K,Oh

    更新日期:2020-07-01 00:00:00

  • Splenic lymphoma presenting as warm autoimmune hemolytic anemia associated with pure red cell aplasia.

    abstract:BACKGROUND AND OBJECTIVE:Warm autoimmune hemolytic anemia (AIHA) is a condition in which peripheral red blood cell (RBC) destruction is induced by the presence of an autoantibody. Pure red cell aplasia (PRCA) represents an isolated process of decreased erythropoiesis. The combination of both is quite rare, with a very ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1159/000031163

    authors: Zeidman A,Fradin Z,Barac Y,Bendayan D,Mittelman M,Orlin J

    更新日期:2000-01-01 00:00:00

  • Evaluation of two-step haemoglobin screening with HemoCue for blood donor qualification in mobile collection sites.

    abstract:BACKGROUND AND OBJECTIVES:Inaccuracy of fingerstick haemoglobin compromises donor's health and losses blood donations. We evaluated the benefit of double haemoglobin screening with HemoCue. STUDY DESIGN AND METHODS:Blood donors underwent fingerstick screening by HemoCue and were driven for donation if capillary haemog...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12165

    authors: Gómez-Simón A,Plaza EM,Torregrosa JM,Ferrer-Marín F,Sánchez-Guiu I,Vicente V,Lozano ML,Rivera J

    更新日期:2014-11-01 00:00:00

  • Modified formulation of CPDA for storage of whole blood, and of SAGM for storage of red blood cells, to maintain the concentration of 2,3-diphosphoglycerate.

    abstract:BACKGROUND AND OBJECTIVES:A dramatic decrease in the level of 2,3-diphosphoglycerate (2,3-DPG) takes place during the storage of whole blood (WB) in CPDA (citrate-phosphate-dextrose-adenine) and a similar decrease occurs during the storage of red blood cells (RBCs) in SAGM (saline-adenine-glucose-mannitol). The aim of ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.0042-9007.2003.00366.x

    authors: Kurup PA,Arun P,Gayathri NS,Dhanya CR,Indu AR

    更新日期:2003-11-01 00:00:00

  • Direct comparison of three residual risk models for hepatitis B virus window period infections using updated input parameters.

    abstract:BACKGROUND AND OBJECTIVES:Comparison of two models for estimating residual transfusion transmission risk by NAT screened window period (WP) donations in South African repeat donors gave identical results for HIV but not for HBV. In order to understand discrepant HBV modelling outcomes, the values of input parameters in...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/vox.12889

    authors: Lelie N,Vermeulen M,van Drimmelen H,Coleman C,Bruhn R,Reddy R,Busch M,Kleinman S

    更新日期:2020-04-01 00:00:00