Abstract：
:During the past 15 years mass spectrometry (MS)-based analyses have become established as the method of choice for direct protein identification and measurement. Owing to the remarkable improvements in the sensitivity and resolution of MS instruments, this technology has revolutionised the opportunities available for the system-wide characterisation of proteins, with wide applications across virtually the whole of cell biology. In this article we provide a perspective on the current state of the art and discuss how the future of cell biology research may benefit from further developments and applications in the field of MS and proteomics, highlighting the major challenges ahead for the community in organising the effective sharing and integration of the resulting data mountain.
journal_name
Trends Cell Bioljournal_title
Trends in cell biologyauthors
Ahmad Y,Lamond AIdoi
10.1016/j.tcb.2013.10.010subject
Has Abstractpub_date
2014-04-01 00:00:00pages
257-64issue
4eissn
0962-8924issn
1879-3088pii
S0962-8924(13)00191-8journal_volume
24pub_type
杂志文章,评审abstract：:During clathrin-mediated endocytosis, dynamin promotes the formation of clathrin-coated vesicles, but its mode of action is unresolved. In a recent study, Macia and colleagues made use of dynasore, a dynamin-specific inhibitor, to show that dynamin plays a dual role in endocytosis: they confirmed that dynamin is invol...
journal_title：Trends in cell biology
pub_type： 杂志文章
doi：10.1016/j.tcb.2006.10.004
更新日期：2006-12-01 00:00:00
abstract：:The separation of chromosomes in anaphase is a precarious step in the cell cycle. The separation is irreversible, and any error can lead to cell death or genetic instability. Chromosome separation is controlled by the protease separase. Here we discuss recent work that has revealed additional layers of separase regula...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2016.07.008
更新日期：2017-01-01 00:00:00
abstract：:In animal species undergoing determinate growth, the making of a full-size adult body requires a series of coordinated growth events culminating in the cessation of growth that precedes sexual maturation. The merger between physiology and genetics now coming to pass in the Drosophila model allows us to decipher these ...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2013.03.005
更新日期：2013-07-01 00:00:00
abstract：:The proteins reggie-1 and reggie-2 were originally discovered in neurons during axon regeneration. Subsequently, they were independently identified as markers of lipid rafts in flotation assays and were hence named flotillins. Since then, reggie/flotillin proteins have been found to be evolutionarily conserved and are...
journal_title：Trends in cell biology
pub_type： 杂志文章
doi：10.1016/j.tcb.2009.10.003
更新日期：2010-01-01 00:00:00
abstract：:The basement membrane (BM), a specialized network of extracellular matrix macromolecules, surrounds epithelial, endothelial, muscle, fat and nerve cells. During development, immune surveillance and disease states ranging from cancer to fibrosis, host cells penetrate the BM by engaging tissue-invasive programs, the ide...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2008.08.007
更新日期：2008-11-01 00:00:00
abstract：:Lymphocyte migration from high endothelial venules into lymphoid organs is mediated by a sequence of interactions between cell adhesion molecules on lymphocytes and those on the vascular endothelial cells that line the vessels. recent studies suggest that the so-called lymphocyte homing receptors and vascular addressi...
journal_title：Trends in cell biology
pub_type： 杂志文章
doi：10.1016/0962-8924(94)90234-8
更新日期：1994-09-01 00:00:00
abstract：:The availability of green fluorescent protein (GFP) as a tracer for observing proteins in living cells has revolutionized cell biology and spurred an intensive search for GFP variants with novel characteristics, additional autofluorescent proteins and alternative techniques of protein labelling. Two recent studies - o...
journal_title：Trends in cell biology
pub_type： 杂志文章
doi：10.1016/s0962-8924(02)02343-7
更新日期：2002-09-01 00:00:00
abstract：:Cyclin-dependent kinase 2 (CDK2) regulates diverse aspects of the mammalian cell cycle. Most cancer cells contain mutations in the pathways that control CDK2, and CDK2 activity has received much attention as a target for cancer therapy. However, a recent report demonstrating that some cancer cells can proliferate with...
journal_title：Trends in cell biology
pub_type： 评论,杂志文章
doi：10.1016/s0962-8924(03)00148-x
更新日期：2003-08-01 00:00:00
abstract：:Membrane trafficking pathways function to sort and transport cargoes to various intracellular compartments and to the plasma membrane. This allows precise spatiotemporal control of processes such as signal transduction, which in turn is crucial for complex cell functions such as cell division, migration and polarity. ...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2011.02.003
更新日期：2011-05-01 00:00:00
abstract：:Diabetes is a metabolic disease affecting nearly 300 million individuals worldwide. Both types of diabetes (1 and 2) are characterized by loss of functional pancreatic β-cell mass causing different degrees of insulin deficiency. The Bcl-2 family has a double-edged effect in diabetes. These proteins are crucial control...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2011.03.001
更新日期：2011-07-01 00:00:00
abstract：:Luke and colleagues have recently attributed a new role to a member of the serpin superfamily of serine proteinase inhibitors. They have used Caenorhabditis elegans to show that an intracellular serpin is crucial for maintaining lysosomal integrity. We examine the role of this firewall in preventing necrosis and attem...
journal_title：Trends in cell biology
pub_type： 杂志文章
doi：10.1016/j.tcb.2007.11.006
更新日期：2008-02-01 00:00:00
abstract：:Why some genes are more popular than others remains an open question, but one example of this phenomenon involves the genes controlling planar cell polarity (PCP), the polarization of cells within a plane of a tissue. Indeed, the so-called 'core' PCP genes such as dishevelled, frizzled, and prickle have been extensive...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2016.12.001
更新日期：2017-05-01 00:00:00
abstract：:Hematopoiesis generally refers to hematopoietic development in fetuses and adults, as well as to hematopoietic stem cell differentiation into progeny lineages. The multiple processes that generate diverse hematopoietic cells have been considered to be unidirectional. However, many reports have recently demonstrated th...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2020.07.006
更新日期：2020-10-01 00:00:00
abstract：:Studies of the HEDGEHOG signalling pathway in Drosophila have revealed a functional link between two genes, cubitus interruptus and patched, whose human homologues are, respectively, a proto-oncogene and a tumour suppressor. While the former has been implicated as a transcription factor, controversy has surrounded the...
journal_title：Trends in cell biology
pub_type： 杂志文章
doi：10.1016/0962-8924(96)84934-8
更新日期：1996-12-01 00:00:00
abstract：:DNA double-strand breaks (DSBs) are the most toxic DNA lesions given their oncogenic potential. Nevertheless, programmed DSBs (prDSBs) contribute to several biological processes. Formation of prDSBs is the 'price to pay' to achieve these essential biological functions. Generated by domesticated PiggyBac transposases, ...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2019.11.005
更新日期：2020-02-01 00:00:00
abstract：:Diverse biological and developmental functions are mediated by signalling through the epidermal growth factor (EGF) receptor. In flies, many different mechanisms are used to control and restrict EGF-R signalling, including ligand processing, ligand variety (an inhibitor as well as activators), transcription and perhap...
journal_title：Trends in cell biology
pub_type： 杂志文章
doi：10.1016/S0962-8924(97)01143-4
更新日期：1997-11-01 00:00:00
abstract：:A cell-free system that mimics mitotic fragmentation of Golgi stacks has provided a working model for the disassembly process. Two distinct pathways, one COP-dependent and one COP-independent, act on Golgi stacks to give rise to two types of end products: transport vesicles and larger, more heterogeneous vesicles and ...
journal_title：Trends in cell biology
pub_type： 杂志文章
doi：10.1016/s0962-8924(00)89094-7
更新日期：1995-11-01 00:00:00
abstract：:In essence, the mitotic cell cycle in eukaryotes involves the duplication and separation of chromosomes, coupled to the process of dividing one cell into two. Cytokinesis is therefore the culmination of a series of events that were triggered during G1 phase, and brings the daughter cells back to the starting position ...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/s0962-8924(00)01765-7
更新日期：2000-06-01 00:00:00
abstract：:Centromere formation is a complex process that involves the packaging of DNA into a centromere-unique chromatin, chemical modification and the seeding of kinetochore and associated proteins. The early steps in this process, in which a chromosomal region is marked for centromerization (that is, to become resolutely com...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/s0962-8924(00)01739-6
更新日期：2000-05-01 00:00:00
abstract：:The light-sensitive outer segment organelle of the vertebrate photoreceptor cell is a modified cilium filled with hundreds of flattened 'disc' membranes that provide vast light-absorbing surfaces. The outer segment is constantly renewed with new discs added at its base every day. This continuous process is essential f...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2020.08.005
更新日期：2020-11-01 00:00:00
abstract：:The ErbB family of four receptor tyrosine kinases occupies a central role in a wide variety of biological processes from neuronal development to breast cancer. New information continues to expand their biologic significance and to unravel the molecular mechanisms that underlie the signaling capacity of these receptors...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2006.10.008
更新日期：2006-12-01 00:00:00
abstract：:The cell surface receptor Fas/APO-1 and its ligand have recently been identified as important mediators of apoptosis. Both molecules are crucial for the maintenance of a sound immune system, and when defective they give rise to severe autoimmune disorders. Understanding the mechanism and regulation of Fas/APO-1-trigge...
journal_title：Trends in cell biology
pub_type： 杂志文章
doi：10.1016/0962-8924(94)90102-3
更新日期：1994-12-01 00:00:00
abstract：:Ubiquinone (UQ; also known as coenzyme Q; CoQ) is a mobile component of the mitochondrial electron transport chain, where it acts as a pro-oxidant in its ubisemiquinone state. Despite this, UQ is also believed to be a membrane antioxidant. These properties place UQ at the center of hotly debated questions about how mi...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2015.12.007
更新日期：2016-05-01 00:00:00
abstract：:The function of caveolae is hotly debated. It now seems clear that caveolae are stable membrane domains that are kept in place by the actin cytoskeleton. However, this stability can be perturbed, leading to caveolar internalization. Caveolae are important in the regulation of various signaling processes, such as nitri...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/s0962-8924(02)00039-9
更新日期：2003-02-01 00:00:00
abstract：:Proteins of the cyclophilin family display two intriguing properties. On the one hand, they are the intracellular receptors for the immunosuppressive drug cyclosporin A (CsA); on the other hand, they function in vitro as enzymes that catalyse slow steps in protein folding. A dissection of the role of CsA in mediating ...
journal_title：Trends in cell biology
pub_type： 杂志文章
doi：10.1016/0962-8924(92)90200-7
更新日期：1992-09-01 00:00:00
abstract：:Like the conductor of an orchestra, the Sec protein translocation channel is the platform needed to bring together the many different players required for the constitutive and obligatory process of protein transport. This conserved membrane channel, termed SecY in bacteria and Sec61 in eukaryotes, creates a ubiquitous...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2011.04.005
更新日期：2011-09-01 00:00:00
abstract：:Id helix-loop-helix proteins function at a general level as positive regulators of cell growth and as negative regulators of cell differentiation. They act as dominant-negative antagonists of other helix-loop-helix transcription factors, which drive cell lineage commitment and differentiation in diverse cell types of ...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：
更新日期：1998-02-01 00:00:00
abstract：:Hypoxia is linked to metastasis; however, how it affects metastatic progression is not clear due to limited consensus in the literature. We posit that this lack of consensus is due to hypoxia being studied using different approaches, such as in vitro, primary tumor, or metastasis assays in an isolated manner. Here, we...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2018.06.008
更新日期：2018-11-01 00:00:00
abstract：:Cell cycle transitions are driven by the periodic oscillations of cyclins, which bind and activate cyclin-dependent kinases (CDKs) to phosphorylate target substrates. Cyclin F uses a substrate recruitment strategy similar to that of the other cyclins, but its associated catalytic activity is substantially different. I...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2012.10.011
更新日期：2013-03-01 00:00:00
abstract：:Epithelial cells comprise the foundation for the majority of organs in the mammalian body, and are the source of approximately 90% of all human cancers. Characteristically, epithelial cells form intercellular adhesions, exhibit apical/basal polarity, and orient their mitotic spindles in the plane of the epithelial she...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2011.06.005
更新日期：2011-12-01 00:00:00
