Abstract:
:The ability to noninvasively image embryonic vascular anatomy in mouse models is an important requirement for characterizing the development of the normal cardiovascular system and malformations in the heart and vascular supply. Photoacoustic imaging, which can provide high resolution non invasive images of the vasculature based upon optical absorption by endogenous hemoglobin, is well suited to this application. In this study, photoacoustic images of mouse embryos were obtained ex vivo and in vivo. The images show intricate details of the embryonic vascular system to depths of up to 10 mm, which allowed whole embryos to be imaged in situ. To achieve this, an all-optical photoacoustic scanner and a novel time reversal image reconstruction algorithm, which provide deep tissue imaging capability while maintaining high spatial resolution and contrast were employed. This technology may find application as an imaging tool for preclinical embryo studies in developmental biology as well as more generally in preclinical and clinical medicine for studying pathologies characterized by changes in the vasculature.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Laufer J,Norris F,Cleary J,Zhang E,Treeby B,Cox B,Johnson P,Scambler P,Lythgoe M,Beard Pdoi
10.1117/1.JBO.17.6.061220subject
Has Abstractpub_date
2012-06-01 00:00:00pages
061220issue
6eissn
1083-3668issn
1560-2281journal_volume
17pub_type
杂志文章abstract::The composition of cartilage is predictive of its in vivo performance. Therefore, the ability to assess its primary macromolecular components, proteoglycan (PG) and collagen, is of great importance. In the current study, we hypothesized that PG content and distribution in tissue engineered cartilage could be determine...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1922329
更新日期:2005-05-01 00:00:00
abstract::We present the application of a microfluidic optical cell stretcher to measure the elasticity of malaria-infected red blood cells. The measurements confirm an increase in host cell rigidity during the maturation of the parasite Plasmodium falciparum. The device combines the selectivity and sensitivity of single-cell e...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3458919
更新日期:2010-05-01 00:00:00
abstract::Cerenkov light is created in clinical applications involving high-energy radiation such as in radiation therapy. There is considerable interest in using Cerenkov light as a means to perform in vivo dosimetry during radiation therapy; however, a better understanding of the light-to-dose relationship is needed. One such...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.23.10.105007
更新日期:2018-10-01 00:00:00
abstract::The ability to quantitatively determine tissue fluorescence is of interest for the purpose of better understanding the details of photodynamic therapy of skin cancer. In particular, we are interested in quantifying protoporphyrin IX (PpIX) in vivo. We present a method of correcting fluorescence for effects of native t...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3665440
更新日期:2011-12-01 00:00:00
abstract::Intraoperative applications of near-infrared (NIR) fluorescent contrast agents can be aided by instrumentation capable of merging the view of surgical field with that of NIR fluorescence. We demonstrate augmented microscopy, an intraoperative imaging technique in which bright-field (real) and electronically processed ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.10.106002
更新日期:2015-10-01 00:00:00
abstract::Robot-assisted laparoscopic surgery is becoming an established technique for prostatectomy and is increasingly being explored for other types of cancer. Linking intraoperative imaging techniques, such as fluorescence guidance, with the three-dimensional insights provided by preoperative imaging remains a challenge. Na...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.8.086008
更新日期:2016-08-01 00:00:00
abstract::We present a novel fluorescence imaging system developed for real-time interventional imaging applications. The system implements a correction scheme that improves the accuracy of epi-illumination fluorescence images for light intensity variation in tissues. The implementation is based on the use of three cameras oper...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3259362
更新日期:2009-11-01 00:00:00
abstract::The main curative therapy for patients with nonsmall cell lung cancer is surgery. Despite this, the survival rate is only 50%, therefore it is important to more efficiently diagnose and predict prognosis for lung cancer patients. Raman spectroscopy is useful in the diagnosis of malignant and premalignant lesions. The ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3323088
更新日期:2010-03-01 00:00:00
abstract::Within the framework of further development of unified Monte Carlo code for the needs of biomedical optics and biophotonics, we present an approach for modeling of coherent polarized light propagation in highly scattering turbid media, such as biological tissues. The temporal coherence of light, linear and circular po...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.2.025005
更新日期:2014-02-01 00:00:00
abstract::To improve surgical guidance toward prostate draining lymph nodes, we investigate the potential of intraoperative fluorescence imaging and combined pre- and intraoperative multimodality imaging approaches. Transgenic adenocarcinoma mouse prostate mice with spontaneous prostate tumors are injected intratumorally with: ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3528027
更新日期:2011-01-01 00:00:00
abstract::The use of reduced nicotinamide adenine dinucleotide (NADH) fluorescence to gain metabolic information on kidneys in response to an alteration in oxygen availability has previously been experimentally demonstrated, but signal quantification has not, to date, been addressed. In this work the relative contribution to ra...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3094948
更新日期:2009-03-01 00:00:00
abstract::The objective of this work was to compare the accuracy of analyte concentration estimation when using transmission versus diffuse reflectance spectroscopy of a scattering medium. Monte Carlo ray tracing of light through the medium was used in conjunction with pure component absorption spectra and Beer-Lambert absorpti...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.23.1.018001
更新日期:2018-01-01 00:00:00
abstract::Multiphoton microscopy (MPM) has the capacity to record second-harmonic generation (SHG) and endogenous two-photon excitation fluorescence (2PEF) signals emitted from biological tissues. The development of fiber-based miniaturized endomicroscopes delivering pulses in the femtosecond range will allow the transfer of MP...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.24.10.106004
更新日期:2019-10-01 00:00:00
abstract::Dihydroartemisinin (DHA), a front-line antimalarial herbal compound, has been shown to possess promising anticancer activity with low toxicity. We have previously reported that DHA induced caspase-3-dependent apoptosis in human lung adenocarcinoma cells. However, the cellular target and molecular mechanism of DHA-indu...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3481141
更新日期:2010-07-01 00:00:00
abstract::Microscopic visualization of islets of Langerhans under normal and diabetic conditions is essential for understanding the pathophysiology of the disease. The intrinsic opacity of pancreata, however, limits optical accessibility for high-resolution light microscopy of islets in situ. Because the standard microtome-base...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3470241
更新日期:2010-07-01 00:00:00
abstract::The epithelium (EP) thickness and the standard deviation (SD) of A-mode scan intensity in the laminar propria (LP) layer are used as effective indicators for the diagnosis of oral submucous fibrosis (OSF) based on the noninvasive clinical scanning of a swept-source optical coherence tomography (OCT) system of approxim...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3233653
更新日期:2009-09-01 00:00:00
abstract::Fourier transform infrared (FTIR) spectroscopic imaging has been used to probe the biochemical composition of human renal tumor tissue and adjacent normal tissue. Freshly resected renal tumor tissue from surgery was prepared as a thin cryosection and examined by FTIR spectroscopic imaging. Tissue types could be discri...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3622292
更新日期:2011-09-01 00:00:00
abstract::So far, the study of the optical clearing is almost always about healthy tissue. However, the ultimate goal is to detect diseases for clinical application. Optical clearing on diseased skins is explored. The effect is evaluated by applying a combined liquid paraffin and glycerol mixed solution on several kinds of dise...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.11.115003
更新日期:2012-11-01 00:00:00
abstract::Cancer is the main cause of canine morbidity and mortality. The existing evaluation of tumors requires an experienced veterinarian and usually includes invasive procedures (e.g., fine-needle aspiration) that can be unpleasant for the dog and the owner. We investigate visible and near-infrared diffuse reflectance spect...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.3.037003
更新日期:2015-03-01 00:00:00
abstract::Dispersion encoded full-range (DEFR) frequency-domain optical coherence tomography (FD-OCT) and its enhanced version, fast DEFR, utilize dispersion mismatch between sample and reference arm to eliminate the ambiguity in OCT signals caused by non-complex valued spectral measurement, thereby numerically doubling the usa...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.7.077007
更新日期:2012-07-01 00:00:00
abstract:SIGNIFICANCE:Adaptive optics optical coherence tomography (AO-OCT) technology enables non-invasive, high-resolution three-dimensional (3D) imaging of the retina and promises earlier detection of ocular disease. However, AO-OCT data are corrupted by eye-movement artifacts that must be removed in post-processing, a proce...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.26.1.016001
更新日期:2021-01-01 00:00:00
abstract::In order to monitor dynamic physiological events in near-real time, a variety of photoacoustic computed tomography (PACT) systems have been developed that can rapidly acquire data. Previously reported studies of dynamic PACT have employed conventional static methods to reconstruct a temporally ordered sequence of imag...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.5.056007
更新日期:2014-05-01 00:00:00
abstract::We present a method for lung cancer detection exploiting reflectance spectra measured in vivo during endoscopic imaging of the lung. The measured reflectance spectra were analyzed using a specially developed light-transport model to obtain quantitative information about cancer-related, physiological, and morphologic c...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2337529
更新日期:2006-07-01 00:00:00
abstract::We present methods for visualizing the dynamic response of biological samples to laser-induced heating. Our approach utilizes optical frequency-domain imaging to detect, spatially localize, and monitor unique dynamic signatures that arise within zones of active tissue denaturation. Since this information is precisely ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2714027
更新日期:2007-03-01 00:00:00
abstract::Myocardial infarction leads to structural remodeling of the myocardium, in particular to the loss of cardiomyocytes due to necrosis and an increase in collagen with scar formation. Stem cell regenerative treatments have been shown to alter this remodeling process, resulting in improved cardiac function. As healthy myo...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3469844
更新日期:2010-07-01 00:00:00
abstract::The results of an experimental study of the possibilities of monitoring erbium yttrium aluminum garnet laser-mediated ablation of human epidermis with the use of Fourier transform infrared (FTIR) spectroscopy and spectral polarization techniques are presented. The attenuated total reflection (ATR) method was used for ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1755719
更新日期:2004-07-01 00:00:00
abstract::We introduce the concept of deliberate placement of absorbers to alter the average path of photons through tissue for a biomedical optical device. By changing the reflectivity of a needle that separates a source and detector, the average photon path through a turbid medium can be changed. Totally reflective needles ha...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2168167
更新日期:2006-01-01 00:00:00
abstract::Frequency-domain diffuse optical spectroscopy (FD-DOS) utilizes intensity-modulated light to characterize optical scattering and absorption in thick tissue. Previous FD-DOS systems have been limited by large device footprints, complex electronics, high costs, and limited acquisition speeds, all of which complicate acc...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.3.036009
更新日期:2017-03-01 00:00:00
abstract::A simple empirical method is presented to determine the scattering coefficient mu' s from backscattered polarized images of turbid media. It uses the ratio, pixel by pixel, of two images that are the second and the first backscattered Stokes parameter images Q and I, respectively. Taking this image ratio, then integra...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1924715
更新日期:2005-05-01 00:00:00
abstract::A method is proposed for determining the glucose concentration on the human fingertip by extracting two optical parameters, namely the optical rotation angle and the depolarization index, using a Mueller optical coherence tomography technique and a genetic algorithm. The feasibility of the proposed method is demonstra...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.23.4.047001
更新日期:2018-04-01 00:00:00