Abstract:
:The use of reduced nicotinamide adenine dinucleotide (NADH) fluorescence to gain metabolic information on kidneys in response to an alteration in oxygen availability has previously been experimentally demonstrated, but signal quantification has not, to date, been addressed. In this work the relative contribution to rat kidney autofluorescence of the capsule versus cortex under ultraviolet excitation is determined from experimental results obtained using autofluorescence microscopy and a suitable mathematical model. The results allow for a quantitative assessment of the relative contribution of the signal originating in the metabolically active cortex as a function of capsule thickness for different wavelengths.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Raman RN,Pivetti CD,Rubenchik AM,Matthews DL,Troppmann C,Demos SGdoi
10.1117/1.3094948subject
Has Abstractpub_date
2009-03-01 00:00:00pages
020505issue
2eissn
1083-3668issn
1560-2281journal_volume
14pub_type
信件abstract::Virus inactivation in red blood cell concentrates (RBCC) is being studied in order to increase the safety of the blood supply. For this purpose we have been studying the silicon phthalocyanine (Pc 4), a photosensitizer activated with red light. Two approaches were used to achieve enhanced selectivity of Pc 4 for virus...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.429931
更新日期:1999-07-01 00:00:00
abstract::A microelectromechanical system (MEMS) mirror based endoscopic swept-source optical coherence tomography (SS-OCT) system that can perform three-dimensional (3-D) imaging at high speed is reported. The key component enabling 3-D endoscopic imaging is a two-axis MEMS scanning mirror which has a 0.8×0.8 mm2 mirror plate...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.8.086005
更新日期:2013-08-01 00:00:00
abstract::In near-infrared spectroscopy, concentration changes in oxygenated and deoxygenated hemoglobin are calculated from the changes in the attenuation of the measurement light. This is done by solving a linear equation based on the modified Lambert-Beer law. To solve this equation, we need to know the partial optical pathl...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3147402
更新日期:2009-05-01 00:00:00
abstract::A side-viewing, 2.3-mm diameter oblique incidence reflectometry endoscope has been designed to obtain optical property measurements of turbid samples. Light from a single-mode fiber is relayed obliquely onto the tissue with a gradient index lens-based distal optics assembly and the resulting diffuse reflectance profil...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.6.067002
更新日期:2014-06-01 00:00:00
abstract::We present a quantitative analysis of dynamic diffuse optical measurements to obtain oxygen saturation of hemoglobin in volume oscillating compartments. We used a phasor representation of oscillatory hemodynamics at the heart rate and respiration frequency to separate the oscillations of tissue concentrations of oxyhe...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.10.101408
更新日期:2016-10-01 00:00:00
abstract::Voltage-sensitive dyes (VSDs) are designed to monitor membrane potential by detecting fluorescence changes in response to neuronal or muscle electrical activity. However, fluorescence imaging is limited by depth of penetration and high scattering losses, which leads to low sensitivity in vivo systems for external dete...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.4.045006
更新日期:2017-04-01 00:00:00
abstract:SIGNIFICANCE:Despite the importance of the cell membrane in regulation of drug activity, the influence of drug treatments on its physical properties is still poorly understood. The combination of fluorescence lifetime imaging microscopy (FLIM) with specific viscosity-sensitive fluorescent molecular rotors allows the qu...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.25.12.126004
更新日期:2020-12-01 00:00:00
abstract::Second-harmonic-generation (SHG) microscopy has emerged as a powerful tool to image unstained living tissues and probe their molecular and supramolecular organization. In this article, we review the physical basis of SHG, highlighting how coherent summation of second-harmonic response leads to the sensitivity of polar...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.JBO.17.6.060901
更新日期:2012-06-01 00:00:00
abstract::Nanobioconjugates have been synthesized using cadmium selenide quantum dots (QDs), europium complexes (EuCs), and biotin. In those conjugates, long-lived photoluminescence (PL) is provided by the europium complexes, which efficiently transfer energy via Förster resonance energy transfer (FRET) to the QDs in close spat...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.10.101506
更新日期:2014-01-01 00:00:00
abstract::We successfully acquire the in vivo Raman spectrum of melanin from human skin using a rapid near-infrared (NIR) Raman spectrometer. The Raman signals of in vivo cutaneous melanin are similar to those observed from natural and synthetic eumelanins. The melanin Raman spectrum is dominated by two intense and broad peaks ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1805553
更新日期:2004-11-01 00:00:00
abstract::Cellularity of the fibrous caps of coronary atheromas, manifested by the infiltration of macrophages (average size, 20 to 30 microm), is thought to weaken the structural integrity of the cap and predispose plaques to rupture. Therefore, an imaging technology capable of identifying macrophages within fibroatheroma caps...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.3290810
更新日期:2010-01-01 00:00:00
abstract::In order to diagnose cancer, a sample must be removed, prepared, and examined under a microscope, which is expensive, invasive, and time consuming. Fiber optic fluorescence endomicroscopy, where an image guide is used to obtain high-resolution images of tissue in vivo, has shown promise as an alternative to convention...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.9.096003
更新日期:2013-09-01 00:00:00
abstract::We combine laser tweezers with custom computer tracking software and robotics to analyze the motility [swimming speed, VCL (curvilinear velocity), and swimming force in terms of escape laser power (Pesc)] and energetics [mitochondrial membrane potential (MP)] of individual sperm. Domestic dog sperm are labeled with a ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2839051
更新日期:2008-01-01 00:00:00
abstract::Dynamic analyses of vibrational motion in cell membranes provide a lot of information on the complex dynamic motilities of a red blood cell (RBC). Here, we present the correlation properties of membrane fluctuation in discocyte and spherocyte RBCs by using quantitative phase microscopy (QPM). Since QPM can provide nan...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3601460
更新日期:2011-07-01 00:00:00
abstract::Laser-induced breakdown spectroscopy (LIBS) is applied to analyze human fingernails using nanosecond laser pulses. Measurements on 45 nail samples are carried out and 14 key species are identified. The elements detected with the present system are: Al, C, Ca, Fe, H, K, Mg, N, Na, O, Si, Sr, Ti as well as CN molecule. ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3574757
更新日期:2011-05-01 00:00:00
abstract::Microcirculation lesion is a common symptom of chronic liver diseases in the form of vasculature deformation and circulation alteration. In acute to chronic liver diseases such as biliary atresia, microcirculation lesion can have an early onset. Detection of microcirculation lesion is meaningful for studying the progr...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.11.116006
更新日期:2014-01-01 00:00:00
abstract::Optical clearing is a well-known phenomenon. It is based on the matching of refractive indices of a bulk material and scattering particles. The same principle is also used in scattering-based optical measurements of different constituents, such as glucose. By registering changes in scattering, it is possible to evalua...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2904957
更新日期:2008-03-01 00:00:00
abstract::Early detection of tissue hypoxia in the intensive care unit is essential for effective treatment. Reduced nicotinamide adenine dinucleotide (NADH) has been suggested to be the most sensitive indicator of tissue oxygenation at the mitochondrial level. However, no experimental evidence comparing the kinetics of changes...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.1.017005
更新日期:2014-01-01 00:00:00
abstract::The effect of glucose on fluorescence of synthesized ZnCdS nanoparticles in the presence of glucose oxidase or in a mixture of glucose oxidase and peroxidase has been investigated. Behavior of fluorescence characteristics of ZnCdS nanoparticles with nonstabilized surface and coated with polymer shell is compared. It h...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.1.011020
更新日期:2014-01-01 00:00:00
abstract::We describe the use of a Teflon-AF liquid core optical fiber (LCOF) geometry to enhance the collection of Raman scattering from the biochemical creatinine, dissolved in water and in urine. At short integration times, where shot noise is most troublesome, the enhanced signal leads to greater accuracy in estimating the ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1917842
更新日期:2005-05-01 00:00:00
abstract::Probing tissue with near-infrared radiation (NIR) simultaneously produces remitted fluorescence and Raman scattering (IE) plus Rayleigh∕Mie light scattering (EE) that noninvasively give chemical and physical information about the materials and objects within. We model tissue as a three-phase system: plasma and red blo...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3625283
更新日期:2011-09-01 00:00:00
abstract::Sinus blockages are a common reason for physician visits, affecting one out of seven people in the United States, and often require medical treatment. Diagnosis in the primary care setting is challenging because symptom criteria (via detailed clinical history) plus objective imaging [computed tomography (CT) or endosc...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.3.035004
更新日期:2015-03-01 00:00:00
abstract::Photodynamic therapy (PDT) is an alternative to radical surgical resection for T1a or nonresectable carcinomas of the gastroesophageal junction. Besides the concentration of the photosensitizer, the light distribution in tissue is responsible for tumor destruction. For this reason, knowledge about the behavior of ligh...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2564793
更新日期:2007-01-01 00:00:00
abstract:PURPOSE:Investigations of foveal aberrations assume circular pupils. However, the pupil becomes increasingly elliptical with increase in visual field eccentricity. We address this and other issues concerning peripheral aberration specification. METHODS:One approach uses an elliptical pupil similar to the actual pupil ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.2.025004
更新日期:2012-02-01 00:00:00
abstract::Focusing light inside highly scattering media is a challenging task in biomedical optical imaging, manipulation, and therapy. A recent invention has overcome this challenge by time reversing ultrasonically encoded diffuse light to an ultrasound-modulated volume inside a turbid medium. In this technique, a photorefract...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.11.110502
更新日期:2013-11-01 00:00:00
abstract::Dispersion encoded full-range (DEFR) frequency-domain optical coherence tomography (FD-OCT) and its enhanced version, fast DEFR, utilize dispersion mismatch between sample and reference arm to eliminate the ambiguity in OCT signals caused by non-complex valued spectral measurement, thereby numerically doubling the usa...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.7.077007
更新日期:2012-07-01 00:00:00
abstract::The results of an experimental study of the possibilities of monitoring erbium yttrium aluminum garnet laser-mediated ablation of human epidermis with the use of Fourier transform infrared (FTIR) spectroscopy and spectral polarization techniques are presented. The attenuated total reflection (ATR) method was used for ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1755719
更新日期:2004-07-01 00:00:00
abstract::Recently, it was shown in measurements carried out on humans that time-resolved near-infrared reflectometry and fluorescence spectroscopy may allow for discrimination of information originating directly from the brain avoiding influence of contaminating signals related to the perfusion of extracerebral tissues. We rep...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3574018
更新日期:2011-04-01 00:00:00
abstract::Under ultraviolet and visible light excitation, melanin is essentially a nonfluorescent substance. This work reports our study on near-infrared (NIR) fluorescence properties of melanins, and explores potential applications of NIR fluorescence techniques for evaluating skin disorders involving melanin. The NIR fluoresc...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2204007
更新日期:2006-05-01 00:00:00
abstract::Intercellular heterogeneity is a key factor in a variety of core cellular processes including proliferation, stimulus response, carcinogenesis, and drug resistance. However, cell-to-cell variability studies at the single-cell level have been hampered by the lack of enabling experimental techniques. We present a measur...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.3.037008
更新日期:2012-03-01 00:00:00