Abstract:
:Optical clearing is a well-known phenomenon. It is based on the matching of refractive indices of a bulk material and scattering particles. The same principle is also used in scattering-based optical measurements of different constituents, such as glucose. By registering changes in scattering, it is possible to evaluate changes in the concentration of a solvent. This work describes the use of optical coherence tomography (OCT) to monitor glucose-induced changes in the optical properties of samples. Intralipid, mouse skin tissue, and mice (C57BL) are used as samples. Differences between in vitro and in vivo measurement conditions, the effect of glucose on the samples' optical properties, as well as possible problems in OCT experiments are discussed. A comparison of OCT signals from mouse skin samples and mice in vivo shows that the intensity of backscattered radiation is stronger in a living animal than in cultured tissue. Moreover, the effect of glucose on the scattering properties is larger in an in vivo case than in an in vitro case. In comparison with tissue, the effect of glucose is the smallest in Intralipid. The results increase the value of using cultured tissue in developing optical sensing techniques.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Kinnunen M,Myllylä R,Vainio Sdoi
10.1117/1.2904957subject
Has Abstractpub_date
2008-03-01 00:00:00pages
021111issue
2eissn
1083-3668issn
1560-2281journal_volume
13pub_type
杂志文章abstract::Optical coherence tomography (OCT) provides more parameters than pure morphology does. In a recent paper [A. Knuettel and M. Boehlau-Godau, J. Biomed. Opt. 5(1) 83-92 (2000)] we have shown that the refractive index (RI) can be evaluated in a localized manner in skin tissue under in vivo conditions. Based on a theory, ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1647544
更新日期:2004-03-01 00:00:00
abstract::A main field in biomedical optics research is diffuse optical tomography, where intensity variations of the transmitted light traversing through tissue are detected. Mathematical models and reconstruction algorithms based on finite element methods and Monte Carlo simulations describe the light transport inside the tis...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.12.126009
更新日期:2012-12-01 00:00:00
abstract::Dispersion encoded full-range (DEFR) frequency-domain optical coherence tomography (FD-OCT) and its enhanced version, fast DEFR, utilize dispersion mismatch between sample and reference arm to eliminate the ambiguity in OCT signals caused by non-complex valued spectral measurement, thereby numerically doubling the usa...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.7.077007
更新日期:2012-07-01 00:00:00
abstract::Using commercially available organic fluorophores, the current applications of Förster (fluorescence) resonance energy transfer (FRET) are limited to about 80 Å. However, many essential activities in cells are spatially and/or temporally dependent on the assembly/disassembly of transient complexes consisting of large-...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.1.011006
更新日期:2012-01-01 00:00:00
abstract::Cellularity of the fibrous caps of coronary atheromas, manifested by the infiltration of macrophages (average size, 20 to 30 microm), is thought to weaken the structural integrity of the cap and predispose plaques to rupture. Therefore, an imaging technology capable of identifying macrophages within fibroatheroma caps...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.3290810
更新日期:2010-01-01 00:00:00
abstract::Optical coherence microscopy (OCM) combines confocal microscopy and optical coherence tomography (OCT) to improve imaging depth and contrast, enabling cellular imaging in human tissues. We aim to investigate OCM for ex vivo imaging of upper and lower gastrointestinal tract tissues, to establish correlations between OC...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3322704
更新日期:2010-01-01 00:00:00
abstract::Renal ischemia that occurs intraoperatively during procedures requiring clamping of the renal artery (such as renal procurement for transplantation and partial nephrectomy for renal cancer) is known to have a significant impact on the viability of that kidney. To better understand the dynamics of intraoperative renal ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.3.035001
更新日期:2013-03-01 00:00:00
abstract::Current techniques to assess lymph node metastases in cancer patients include lymphoscintigraphy after administration of a nonspecific radiocolloid in order to locate and resect lymph nodes for pathological examination of harbored cancer cells. Clinical trials involving intradermal or subcutaneous injection of antibod...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.2953498
更新日期:2008-07-01 00:00:00
abstract::Multiphoton microscopy (MPM) has the capacity to record second-harmonic generation (SHG) and endogenous two-photon excitation fluorescence (2PEF) signals emitted from biological tissues. The development of fiber-based miniaturized endomicroscopes delivering pulses in the femtosecond range will allow the transfer of MP...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.24.10.106004
更新日期:2019-10-01 00:00:00
abstract::Micrometer scale resolution full-field optical coherence tomography (FF-OCT) is developed for imaging human graft corneas. Three-dimensional (3-D) images with ultrahigh resolution (respectively, 1 and 1.5 μm in the axial and transverse directions), comparable to traditional histological sections, are obtained allowing...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3486544
更新日期:2010-09-01 00:00:00
abstract::Parametric and nonparametric data processing schemes for analyzing translating laser speckle data used to investigate the mechanical behavior of biological tissues are examined. Cross-correlation, minimum mean square estimator, maximum likelihood, and maximum entropy approaches are discussed and compared on speckle da...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1412224
更新日期:2001-10-01 00:00:00
abstract::The Nikon C1 confocal laser scanning microscope is a relatively inexpensive and user-friendly instrument. We describe a straightforward method to convert the C1 for multiphoton microscopy utilizing direct coupling of a femtosecond near-infrared laser into the scan head and fiber optic transmission of emission light to...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3139850
更新日期:2009-05-01 00:00:00
abstract::Clustering analysis (CA) and principal component analysis (PCA) were applied to dynamic Cerenkov luminescence images (dCLI). In order to investigate the performances of the proposed approaches, two distinct dynamic data sets obtained by injecting mice with (32)P-ATP and (18)F-FDG were acquired using the IVIS 200 optic...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3663442
更新日期:2011-12-01 00:00:00
abstract::So far, the study of the optical clearing is almost always about healthy tissue. However, the ultimate goal is to detect diseases for clinical application. Optical clearing on diseased skins is explored. The effect is evaluated by applying a combined liquid paraffin and glycerol mixed solution on several kinds of dise...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.11.115003
更新日期:2012-11-01 00:00:00
abstract::Cardiac architecture is inherently three-dimensional, yet most characterizations rely on two-dimensional histological slices or dissociated cells, which remove the native geometry of the heart. We previously developed a method for labeling intact heart sections without dissociation and imaging large volumes while pres...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3200939
更新日期:2009-07-01 00:00:00
abstract::A confocal imaging and image processing scheme is introduced to visualize and evaluate the spatial distribution of spectral information in tissue. The image data are recorded using a confocal laser-scanning microscope equipped with a detection unit that provides high spectral resolution. The processing scheme is based...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.10.106011
更新日期:2012-10-01 00:00:00
abstract::Optical resolution photoacoustic microscopy (ORPAM) is important for various biomedical applications, such as the study of cellular structures, microcirculation systems, and tumor angiogenesis. However, the lateral resolution of a conventional ORPAM is limited by optical diffraction. In this work, we report a simulati...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.11.116003
更新日期:2014-01-01 00:00:00
abstract::Fast estimation of optical properties from reflectance measurements at two spatial frequencies could pave way for real-time, wide-field and quantitative mapping of vital signs of tissues. We present a machine learning-based approach for estimating optical properties in the spatial frequency domain, where a random fore...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.24.7.071606
更新日期:2018-12-01 00:00:00
abstract::Raman spectroscopy on single, living epithelial cells captured in a laser trap is shown to have diagnostic power over colorectal cancer. This new single-cell technology comprises three major components: primary culture processing of human tissue samples to produce single-cell suspensions, Raman detection on singly tra...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2748060
更新日期:2007-05-01 00:00:00
abstract::Three-dimensional (3-D) tissue imaging offers substantial benefits to a wide range of biomedical investigations from cardiovascular biology, diabetes, Alzheimer's disease to cancer. Two-photon tissue cytometry is a novel technique based on high-speed multiphoton microscopy coupled with automated histological sectionin...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2435626
更新日期:2007-01-01 00:00:00
abstract::Radiotherapy is one of the main methods to treat cancer. However, due to the propagation pattern of high-energy photons in tissue and their inability to discriminate between healthy and malignant tissues, healthy tissues may also be damaged, causing undesired side effects. A possible method for internal electron thera...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.5.051041
更新日期:2015-05-01 00:00:00
abstract::In order to monitor dynamic physiological events in near-real time, a variety of photoacoustic computed tomography (PACT) systems have been developed that can rapidly acquire data. Previously reported studies of dynamic PACT have employed conventional static methods to reconstruct a temporally ordered sequence of imag...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.5.056007
更新日期:2014-05-01 00:00:00
abstract::Near-infrared spectroscopy is a novel imaging technique potentially sensitive to both brain hemodynamics (slow signal) and neuronal activity (fast optical signal, FOS). The big challenge of measuring FOS noninvasively lies in the presumably low signal-to-noise ratio. Thus, detectability of the FOS has been controversi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3505007
更新日期:2010-11-01 00:00:00
abstract::Fourier domain (FD) techniques have increasingly gained attention in optical coherence tomography (OCT). This is primarily due to their demonstrated sensitivity of two to three orders of magnitude over conventional time-domain techniques. FDOCT images are subject to two primary sources of artifacts. First, a complex c...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2136147
更新日期:2005-11-01 00:00:00
abstract::The present work describes the fabrication and characterization of an optical fiber pH sensor using a sol–gel technique. The sensing head configuration is incorporated using a short section of no-core fiber, coated with tetraethyl orthosilicate and spliced at the end of a single mode fiber with a bulge. Different type...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.5.057001
更新日期:2017-05-01 00:00:00
abstract::Most ovarian cancers are diagnosed at advanced stages due to the lack of efficacious screening techniques. Photoacoustic tomography (PAT) has a potential to image tumor angiogenesis and detect early neovascular changes of the ovary. We have developed a coregistered PAT and ultrasound (US) prototype system for real-tim...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.4.046006
更新日期:2016-04-30 00:00:00
abstract::Optical coherence tomography (OCT) is an emerging technology for high-resolution, noncontact imaging of transparent and scattering media. Polarization-sensitive optical coherence tomography (PS-OCT) is a functional extension of OCT that can image birefringent properties of a biological sample. PS-OCT was used to measu...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1629308
更新日期:2004-01-01 00:00:00
abstract::We present methods for visualizing the dynamic response of biological samples to laser-induced heating. Our approach utilizes optical frequency-domain imaging to detect, spatially localize, and monitor unique dynamic signatures that arise within zones of active tissue denaturation. Since this information is precisely ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2714027
更新日期:2007-03-01 00:00:00
abstract::The present report addresses the application of surface plasmon resonance (SPR) phenomenon for urea sensing. The former promises a high sensitivity, label-free detection, and real-time information by monitoring the refractive index change at the metal–dielectric interface. In the present report, a highly sensitive ure...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.8.087006
更新日期:2016-08-01 00:00:00
abstract::Penetration profiles of topically applied drugs and cosmetic products provide important information on their efficacy. The application of tape stripping in combination with UV/VIS spectroscopy is checked to determine the local position of topically applied substances inside the stratum corneum, the penetration profile...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1854683
更新日期:2005-01-01 00:00:00