Abstract:
:We present methods for visualizing the dynamic response of biological samples to laser-induced heating. Our approach utilizes optical frequency-domain imaging to detect, spatially localize, and monitor unique dynamic signatures that arise within zones of active tissue denaturation. Since this information is precisely registered with high-resolution ( approximately 10 microm) cross sectional images, regions of thermally destroyed tissue can be mapped in relation to pre-existing morphology. Using porcine esophageal specimens ex vivo, we demonstrate that the extent and evolution of laser thermal damage can be assessed in real time.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Vakoc BJ,Tearney GJ,Bouma BEdoi
10.1117/1.2714027subject
Has Abstractpub_date
2007-03-01 00:00:00pages
020501issue
2eissn
1083-3668issn
1560-2281journal_volume
12pub_type
杂志文章abstract::Photospallation is proposed as the primary mechanism behind our recent animal studies involving corneal ablation by nanosecond-pulse mid-IR laser beams. Following a brief summary of earlier work directed to refractive procedures in the mid-IR, a preliminary analysis is presented, based on simple one-dimensional models...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.429958
更新日期:1999-10-01 00:00:00
abstract::Two pronounced absorption peaks in blue and red ranges of the chlorin-based photosensitizer (PS) absorption spectrum provide additional benefits in photodynamic therapy (PDT) performance. Differing optical properties of biological tissues in these ranges allow for both dual-wavelength diagnostics and PDT performance. ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.25.6.063804
更新日期:2019-12-01 00:00:00
abstract::Autofluorescence of the cornea within specific wavelength region and dynamic light scattering measurements of ocular tissue have both been used for early-stage detection of the presence of diabetic retinopathy. In the present study, autofluorescence of the cornea and dynamic light scattering in the cornea and vitreous...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.429882
更新日期:1998-07-01 00:00:00
abstract::A simultaneous multispectral fluorescence imaging system incorporating multiplexed volume holographic grating (VHG) is developed to acquire multispectral images of an object in one shot. With the multiplexed VHG, the imaging system can provide the distribution and spectral characteristics of multiple fluorophores in t...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.6.060502
更新日期:2016-06-01 00:00:00
abstract::Given the several difficulties associated with histology, including difficulty in continuous monitoring, this study aimed to investigate the feasibility of optical imaging modalities—cross-polarization color (CPC) imaging, erythema index (EI) imaging, and laser speckle contrast (LSC) imaging—for continuous evaluation ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.10.106010
更新日期:2014-01-01 00:00:00
abstract::Spectrally resolved two-photon excited autofluorescence imaging is used to distinguish different cell types and functional areas during dynamic processes in the living gut. Excitation and emission spectra of mucosal tissue and tissue components are correlated to spectra of endogenous chromophores. We show that selecti...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3655587
更新日期:2011-11-01 00:00:00
abstract::We instrumented a combined fluorescence spectroscopy and imaging system to characterize the single- and two-photon excited autofluorescence in epithelial tissue. Single-photon fluorescence (SPF) are compared with two-photon fluorescence (TPF) measured at the same location in epithelial tissue. It was found that the SP...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2975866
更新日期:2008-09-01 00:00:00
abstract::In part I of the study, two attachment systems [O-ring; bar-clip (BC)] were used, and the system with three individualized O-rings provided the lowest stress on the implants and the support tissues. Therefore, the aim of this study was to assess the stress distribution, through the photoelastic method, on implant-reta...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.6.066012
更新日期:2014-06-01 00:00:00
abstract::This paper presents a fiber Bragg grating (FBG)-instrumented prosthetic silicone liner that provides cushioning for the residual limb and can successfully measure interface pressures inside prosthetic sockets of lower-limb amputees in a simple and practical means of sensing. The liner is made of two silicone layers be...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.8.087001
更新日期:2017-08-01 00:00:00
abstract::Fluorescence image-guided surgery (FIGS) is an emerging technique in oncology, neurology, and cardiology. To adapt intraoperative imaging for various surgical applications, increasingly flexible and compact FIGS instruments are necessary. We present a compact, portable FIGS system and demonstrate its use in cardiovasc...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3378128
更新日期:2010-03-01 00:00:00
abstract::Sinus blockages are a common reason for physician visits, affecting one out of seven people in the United States, and often require medical treatment. Diagnosis in the primary care setting is challenging because symptom criteria (via detailed clinical history) plus objective imaging [computed tomography (CT) or endosc...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.3.035004
更新日期:2015-03-01 00:00:00
abstract::We examine the morphological and chemical changes in the pulp chamber dentin after using endodontic agents by scanning electron microscopy (SEM), Fourier transform Raman spectroscopy (FT-Raman), and micro energy-dispersive x-ray fluorescence spectrometry (μEDXRF). Thirty teeth were sectioned exposing the pulp chamber ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.7.075008
更新日期:2012-07-01 00:00:00
abstract::Polarized Raman spectroscopy allows measurement of molecular orientation and composition and is widely used in the study of polymer systems. Here, we extend the technique to the extraction of quantitative orientation information from bone tissue, which is optically thick and highly turbid. We discuss multiple scatteri...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3426310
更新日期:2010-05-01 00:00:00
abstract::The main curative therapy for patients with nonsmall cell lung cancer is surgery. Despite this, the survival rate is only 50%, therefore it is important to more efficiently diagnose and predict prognosis for lung cancer patients. Raman spectroscopy is useful in the diagnosis of malignant and premalignant lesions. The ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3323088
更新日期:2010-03-01 00:00:00
abstract::Surgical light is important for helping the surgeon easily identify specific tissues during an operation. We propose a spectral reflectance comparison model to optimize the light-emitting diode light spectrum in the operating room. An entropy evaluation method, meant specifically for surgical situations, was developed...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.10.105012
更新日期:2015-10-01 00:00:00
abstract::When irradiated with nanosecond laser pulses, gold nanoparticles allow for manipulation or destruction of cells and proteins with high spatial and temporal precision. Gold nanorods are especially attractive, because they have an up-to-20-fold stronger absorption than a sphere of equal volume, which is shifted to the o...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.5.058003
更新日期:2012-05-01 00:00:00
abstract::In order to evaluate the impact of anatomy on the spectral properties of oral tissue, we used reflectance and fluorescence spectroscopy to characterize nine different anatomic sites. All spectra were collected in vivo from healthy oral mucosa. We analyzed 710 spectra collected from the oral cavity of 79 healthy volunt...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2992139
更新日期:2008-11-01 00:00:00
abstract::We have developed a novel nitroimidazole indocyanine dye conjugate for tumor-targeted hypoxia fluorescence tomography. The hypoxia probe has been evaluated in vitro using tumor cell lines and in vivo with tumor targeting in mice. The in vitro cell studies were performed to assess fluorescence labeling differences betw...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3589348
更新日期:2011-06-01 00:00:00
abstract::Optical coherence tomography (OCT) is a noninvasive imaging methodology that is able to image tissue to depths of over 1 mm. Many epithelial conditions, such as melanoma and oral cancers, require an invasive biopsy for diagnosis. A noninvasive, real-time, point of care method of imaging depth-resolved epithelial struc...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3652708
更新日期:2011-11-01 00:00:00
abstract::Imaging technologies working in the spatial frequency domain are becoming increasingly popular for generating wide-field maps of optical properties, enabling rapid analysis of tissue parameters. While acquisition methods have become faster and are now performing in real-time, processing methods remain slow, precluding...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.11.110501
更新日期:2016-11-01 00:00:00
abstract::Vascular leakage is an important feature of various disease conditions. In vivo optical imaging provides a great opportunity for the evaluation of this phenomenon. In the present study, we focus on the development and validation of a near-infrared (NIR) imaging formula to allow reliable, cost-efficient evaluation of v...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.1.016022
更新日期:2015-01-01 00:00:00
abstract::A new, simple, high-speed, and hardware-only integration-based fluorescence-lifetime-sensing algorithm using a center-of-mass method (CMM) is proposed to implement lifetime calculations, and its signal-to-noise-ratio based on statistics theory is also deduced. Compared to the commonly used iterative least-squares meth...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3309737
更新日期:2010-01-01 00:00:00
abstract::We assess the properties of contact pressure applied by manually operated fiber-optic probes as a function of the operator, probe contact area, and sample stiffness. First, the mechanical properties of human skin sites with different skin structures, thicknesses, and underlying tissues were studied by in vivo indentat...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.12.127002
更新日期:2015-01-01 00:00:00
abstract::A three-beam spectral domain optical coherence tomography system (OCT) whose center wavelength is 840 nm was developed. The three beams focus on fundus 3.1 mm apart from each other and are detected by a single line sensor. The distance between the beams is fixed and the beams scan a total area of 10×10 mm² while keep...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.10.106001
更新日期:2012-10-01 00:00:00
abstract::Laser speckle contrast imaging is a technique used for imaging blood flow without scanning. Though several studies have attempted to combine spatial and temporal statistics of laser speckle images for reducing image noise as well as preserving acceptable spatiotemporal resolution, the statistical accuracy of these spa...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3290804
更新日期:2010-01-01 00:00:00
abstract::As the most abundant cell type in the central nervous system, astrocyte has been one of main research topics in neuroscience. Although various tools have been developed, at present, there is no tool that allows noninvasive activation of astrocyte in vivo without genetic or pharmacological perturbation. Here we report ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3600774
更新日期:2011-07-01 00:00:00
abstract::We have combined a confocal laser scanning head modified for TPE (two-photon excitation) microscopy with some spectroscopic modules to study single molecules and molecular aggregates. The behavior of the TPE microscope unit has been characterized by means of point spread function measurements and of the demonstration ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.1382809
更新日期:2001-07-01 00:00:00
abstract::In a previous study, we investigated physical methods to reduce whole-body, diet-related autofluorescence interference in several mouse strains through changes in animal diet. Measurements of mice with an in vivo multispectral imaging system over a 21-day period allowed for the quantification of concentration changes ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2799188
更新日期:2007-09-01 00:00:00
abstract::Micrometer scale resolution full-field optical coherence tomography (FF-OCT) is developed for imaging human graft corneas. Three-dimensional (3-D) images with ultrahigh resolution (respectively, 1 and 1.5 μm in the axial and transverse directions), comparable to traditional histological sections, are obtained allowing...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3486544
更新日期:2010-09-01 00:00:00
abstract::Shallow lesions less than 1.5-cm deep are frequently seen in breast patients when they are scanned in reflection geometry. Two boundary conditions are compared for imaging shallow lesions, and a new probe design is introduced. A partial reflection boundary condition is suitable for imaging shallow lesions less than 1....
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2966703
更新日期:2008-07-01 00:00:00