Abstract:
:Three-dimensional (3-D) tissue imaging offers substantial benefits to a wide range of biomedical investigations from cardiovascular biology, diabetes, Alzheimer's disease to cancer. Two-photon tissue cytometry is a novel technique based on high-speed multiphoton microscopy coupled with automated histological sectioning, which can quantify tissue morphology and physiology throughout entire organs with subcellular resolution. Furthermore, two-photon tissue cytometry offers all the benefits of fluorescence-based approaches including high specificity and sensitivity and appropriateness for molecular imaging of gene and protein expression. We use two-photon tissue cytometry to image an entire mouse heart at subcellular resolution to quantify the 3-D morphology of cardiac microvasculature and myocyte morphology spanning almost five orders of magnitude in length scales.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Ragan T,Sylvan JD,Kim KH,Huang H,Bahlmann K,Lee RT,So PTdoi
10.1117/1.2435626subject
Has Abstractpub_date
2007-01-01 00:00:00pages
014015issue
1eissn
1083-3668issn
1560-2281journal_volume
12pub_type
杂志文章abstract::This study investigates the precision and intraindividual variability of a clinical optical pachometer based on low-coherence reflectometry, which was used to measure the central thickness of a human cornea in vivo. The instrument, attached to a slit lamp, is a single mode fiber optic based Michelson interferometer wi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.429901
更新日期:1999-01-01 00:00:00
abstract::We present the first experimental result of direct delineation of the nuclei of living rat bladder epithelium with ultrahigh-resolution optical coherence tomography (uOCT). We demonstrate that the cellular details embedded in the speckle noise in a uOCT image can be uncovered by time-lapse frame averaging that takes a...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.2800007
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abstract::For clinical optoacoustic imaging, linear probes are preferably used because they allow versatile imaging of the human body with real-time display and free-hand probe guidance. The two-dimensional (2-D) optoacoustic image obtained with this type of probe is generally interpreted as a 2-D cross-section of the tissue ju...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.2.026011
更新日期:2013-02-01 00:00:00
abstract::A side-viewing, 2.3-mm diameter, surface magnifying chromoendoscopy-optical coherence tomography (SMC-OCT) endoscope has been designed for simultaneous, nondestructive surface fluorescence visualization and cross-sectional imaging. We apply this endoscope to in vivo examination of the mouse colon. A 30,000 element fib...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.8.086003
更新日期:2012-08-01 00:00:00
abstract::An integrated miniature multi-modal microscope (4M device) for microendoscopy was built and tested. Imaging performance is evaluated and imaging results are presented for both fluorescence and reflectance samples. Images of biological samples show successful imaging of both thin layers of fixed cells prepared on a sli...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2978060
更新日期:2008-09-01 00:00:00
abstract::Ultrasmall scale implants have recently attracted focus as valuable tools for monitoring both acute and chronic diseases. Semiconductor optical technologies are the key to miniaturizing these devices to the long-sought sub-mm scale, which will enable long-term use of these devices for medical applications. This can al...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.9.095012
更新日期:2015-09-01 00:00:00
abstract::It is widely known that not all of the treated cells survive after introduction of exogenous molecules via any physical method. Therefore, it is important to develop methods that would allow simultaneous evaluation of both molecular delivery efficiency and cell viability. This study presents Förster resonance energy t...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.9.097001
更新日期:2017-09-01 00:00:00
abstract::We fabricate scaffolds with a fine linear grating (periodicity of 1 to 8 microm and height of 1 microm) on a glass substrate for controlling cardiomyocyte orientation. The fabrication is done by the solidification of a liquid photopolymerizable material using two laser beam interference. As the photopolymerizable mate...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2186042
更新日期:2006-03-01 00:00:00
abstract::We present a multimodal optical microscope that incorporates six imaging modalities on one common platform. The imaging modalities include three staring modes, optical quadrature microscopy (OQM), differential interference contrast (DIC) microscopy, and epi-fluorescence microscopy, and three scanning modes, confocal r...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2753744
更新日期:2007-07-01 00:00:00
abstract:SIGNIFICANCE:Detailed biochemical and morphological imaging of the plaque burdened coronary arteries holds the promise of improved understanding of atherosclerosis plaque development, ultimately leading to better diagnostics and therapies. AIM:Development of a dual-modality intravascular catheter supporting swept-sour...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.25.9.096010
更新日期:2020-09-01 00:00:00
abstract::Spectral resolved tissue imaging has a broad range of biomedical applications such as the minimally invasive diagnosis of diseases and the study of wound healing and tissue engineering processes. Two-photon microscopy imaging of endogenous fluorescence has been shown to be a powerful method for the quantification of t...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1891370
更新日期:2005-03-01 00:00:00
abstract::We present a new detection instrument for sensor measurements based on excited-state fluorescence lifetimes. This system consists of a primary optoelectronic loop containing a resonance-type rf amplifier, a modulatable fluorescence-excitation light source, a fiber optic feedback loop (with a gap for a fluorescent sens...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1688814
更新日期:2004-05-01 00:00:00
abstract::We investigate a technology to create a high temperature heat source on the tip surface of the glass fiber proposed for medical surgery applications. Using 4 to 6 W power level semiconductor lasers at a wavelength of 980 nm, a laser coupled fiber tip was preprocessed to contain a certain amount of titanium oxide powde...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.10.101502
更新日期:2014-01-01 00:00:00
abstract::Autofluorescence of the cornea within specific wavelength region and dynamic light scattering measurements of ocular tissue have both been used for early-stage detection of the presence of diabetic retinopathy. In the present study, autofluorescence of the cornea and dynamic light scattering in the cornea and vitreous...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.429882
更新日期:1998-07-01 00:00:00
abstract::Oxidative stress has become an exciting area of schizophrenia (SCZ) research, and provides ample opportunities and hope for a better understanding of its pathophysiology, which may lead to new treatment strategies. The first objective of the present study was to analyze the oxidative stress markers in breath samples o...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.5.057006
更新日期:2015-05-01 00:00:00
abstract::We report an accurate optical differentiation technique between healthy and malaria-infected erythrocytes by quasi-simultaneous measurements of transmittance, reflectance, and scattering properties of unstained blood smears using a multispectral and multimode light-emitting diode microscope. We propose a technique for...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.3.036002
更新日期:2013-03-01 00:00:00
abstract::Environmental metastability of zirconia (ZrO2) ceramic in the human body [represented by a tetragonal-to-monoclinic (t→m) phase transformation] takes place on the surface of the artificial joint and proceeds with time toward its interior. Its quantitative characterization is mandatory for the safety of joint implants ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.12.127002
更新日期:2013-12-01 00:00:00
abstract::Dynamic analyses of vibrational motion in cell membranes provide a lot of information on the complex dynamic motilities of a red blood cell (RBC). Here, we present the correlation properties of membrane fluctuation in discocyte and spherocyte RBCs by using quantitative phase microscopy (QPM). Since QPM can provide nan...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3601460
更新日期:2011-07-01 00:00:00
abstract::The lymphatic system is responsible for fluid homeostasis and immune cell trafficking and has been implicated in several diseases, including obesity, diabetes, and cancer metastasis. Despite its importance, the lack of suitable in vivo imaging techniques has hampered our understanding of the lymphatic system. This is,...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.10.106003
更新日期:2017-10-01 00:00:00
abstract::Polarized Raman spectroscopy allows measurement of molecular orientation and composition and is widely used in the study of polymer systems. Here, we extend the technique to the extraction of quantitative orientation information from bone tissue, which is optically thick and highly turbid. We discuss multiple scatteri...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3426310
更新日期:2010-05-01 00:00:00
abstract::A three-beam spectral domain optical coherence tomography system (OCT) whose center wavelength is 840 nm was developed. The three beams focus on fundus 3.1 mm apart from each other and are detected by a single line sensor. The distance between the beams is fixed and the beams scan a total area of 10×10 mm² while keep...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2012-10-01 00:00:00
abstract::Blood, being a suspension of deformable red cells suspended in plasma, displays flow dynamics considerably more complicated than those of an ideal Newtonian fluid. Flow dynamics in blood capillaries of a few hundred micrometers in diameter are investigated using Doppler optical coherence tomography (DOCT) and Doppler ...
journal_title:Journal of biomedical optics
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abstract::The optical activity of glucose molecules is important for diagnosing and monitoring blood glucose of diabetes. In order to accurately detect the diabetes at an early stage, there is an urgent need to develop innovative detection methods. By use of Mueller matrix decomposition (MMD), we have studied the optical activi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.4.046015
更新日期:2014-04-01 00:00:00
abstract::Focusing light inside highly scattering media is a challenging task in biomedical optical imaging, manipulation, and therapy. A recent invention has overcome this challenge by time reversing ultrasonically encoded diffuse light to an ultrasound-modulated volume inside a turbid medium. In this technique, a photorefract...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.11.110502
更新日期:2013-11-01 00:00:00
abstract::Hemoglobin (Hb), as one of main components of blood, has a unique quaternary structure. Its release of oxygen is controlled by oxygen partial pressure (PO2). We investigate the specific spectroscopic changes in Hb under different PO2 levels to optimize clinical methods of measuring tissue PO2. The transmissivity of Hb...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.12.125002
更新日期:2012-12-01 00:00:00
abstract::Metal nanoparticles can be functionalized with biomolecules to selectively localize in precancerous tissues and can act as optical contrast enhancers for reflectance-based diagnosis of epithelial precancer. We carry out Monte Carlo (MC) simulations to analyze photon propagation through nanoparticle-labeled tissues and...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3608999
更新日期:2011-08-01 00:00:00
abstract::Malaria parasites induce morphological, biochemical, and mechanical changes in red blood cells (RBCs). Mechanical variations are closely related to the deformability of individual RBCs. The deformation of various RBCs, including healthy and malaria-infected RBCs (iRBCs), can be directly observed through quantitative p...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.11.115003
更新日期:2015-11-01 00:00:00
abstract::In the present applications of optical coherence tomography (OCT), parameters besides pure morphology are evaluated in skin tissue under in vivo conditions. Spatially mapped refractive indices and scattering coefficients may support tissue characterization for research and diagnostic purposes in cosmetics/pharmacy and...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.429972
更新日期:2000-01-01 00:00:00
abstract::Different colors of visible light penetrate to varying depths in tissue due to the wavelength dependence of tissue optical absorption and elastic scattering. We exploit this to map the contour of the closest surface of a buried fluorescent object. This uses a novel algorithm based on the diffusion theory description o...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2010-11-01 00:00:00
abstract::Alcohol testing is an expanding area of interest due to the impacts of alcohol abuse that extend well beyond drunk driving. However, existing approaches such as blood and urine assays are hampered in some testing environments by biohazard risks. A noninvasive, in vivo spectroscopic technique offers a promising alterna...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2009-09-01 00:00:00