Abstract:
:Different colors of visible light penetrate to varying depths in tissue due to the wavelength dependence of tissue optical absorption and elastic scattering. We exploit this to map the contour of the closest surface of a buried fluorescent object. This uses a novel algorithm based on the diffusion theory description of light propagation in tissue at each excitation wavelength to derive metrics that define the depth of the top surface of the object. The algorithm was validated using a tissue-simulating phantom. It was then demonstrated in vivo by subsurface brain tumor topography in a rodent model, using the fluorescence signal from protoporphyrin IX that is preferentially synthesized within malignant cells following systemic application of aminolevulinic acid. Comparisons to histomorphometry in the brain post mortem show the spatial accuracy of the technique. This method has potential for fluorescence image-guided tumor surgery, as well as other biomedical and nonbiological applications in subsurface sensing.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Kim A,Roy M,Dadani FN,Wilson BCdoi
10.1117/1.3523369subject
Has Abstractpub_date
2010-11-01 00:00:00pages
066026issue
6eissn
1083-3668issn
1560-2281journal_volume
15pub_type
杂志文章abstract::Optical technologies may be applied to multiple facets of spinal surgery from diagnostics to intraoperative image guidance to therapeutics. In diagnostics, the current standard remains cross-sectional static imaging. Optical surface scanning tools may have an important role; however, significant work is required to cl...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.JBO.23.6.060601
更新日期:2018-06-01 00:00:00
abstract::Spectral resolved tissue imaging has a broad range of biomedical applications such as the minimally invasive diagnosis of diseases and the study of wound healing and tissue engineering processes. Two-photon microscopy imaging of endogenous fluorescence has been shown to be a powerful method for the quantification of t...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1891370
更新日期:2005-03-01 00:00:00
abstract::Mid-IR semiconductor lasers of two wavelength bands, 5.4 and 9.6 microm, are applied to measure aqueous glucose concentration ranging from 0 to 500 mgdL with Intralipid emulsion (0 to 8%) added as a fat simulator. The absorption coefficient micro(a) is found linear with respect to glucose and Intralipid concentrations...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2714283
更新日期:2007-03-01 00:00:00
abstract::Intraoperative fluorescence molecular imaging based on targeted fluorescence agents is an emerging approach to improve surgical and endoscopic imaging and guidance. Short exposure times per frame and implementation at video rates are necessary to provide continuous feedback to the physician and avoid motion artifacts....
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.4.046012
更新日期:2014-04-01 00:00:00
abstract::A transgenic mouse model with a Clim [co-factor of LIM (a combination of first letters of Lin-11 (C. elegans), ISL1 (rat), and Mec-3 (C. elegans) gene names) domain proteins] gene partially blocked in the epithelial compartment of its tissues is used to establish the sensitivity of intrinsic reflectance nonlinear opti...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2163254
更新日期:2006-01-01 00:00:00
abstract::A new imaging approach, structured light scatteroscopy (SLS), is demonstrated, which offers rapid wide-field imaging of microscopic morphological variations in bulk tissue surfaces. Elastic scattering of light offers exquisite sensitivity to ultrastructural changes at multiple size scales ranging from nanometers to mi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.7.070504
更新日期:2014-01-01 00:00:00
abstract::Skin cancer diagnosis depends not only on histopathological examination but also on visual inspection before and after the excision of suspected lesion. Neoplasm is accompanied with changes in birefringence of collagen, pleomorphicity, and hyperchromatic state of epithelial nuclei. These phenomena can be measured by s...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3609003
更新日期:2011-08-01 00:00:00
abstract::Discrimination of benign and malignant melanocytic lesions is a major issue in clinical dermatology. Assessment of the thickness of melanoma is critical for prognosis and treatment selection. We aimed to evaluate a novel optical computed tomography (optical-CT) system as a tool for three-dimensional (3-D) imaging of m...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.6.066004
更新日期:2012-06-01 00:00:00
abstract::An algorithm was developed to obtain depth-resolved local optical axis in birefringent samples by using conventional polarization-sensitive optical coherence tomography (PSOCT) that uses a single circularly polarized incident light. The round-trip sample Jones matrices were first constructed from the cumulative PSOCT ...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.JBO.17.11.110501
更新日期:2012-11-01 00:00:00
abstract::Renal ischemia that occurs intraoperatively during procedures requiring clamping of the renal artery (such as renal procurement for transplantation and partial nephrectomy for renal cancer) is known to have a significant impact on the viability of that kidney. To better understand the dynamics of intraoperative renal ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.3.035001
更新日期:2013-03-01 00:00:00
abstract::Bacterial contamination of blood products is one of the most frequent infectious complications of transfusion. Since glucose levels in blood supplies decrease as bacteria proliferate, it should be possible to detect the presence of bacterial contamination by measuring the glucose concentrations in the blood components...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.1.017004
更新日期:2012-01-01 00:00:00
abstract::We theoretically investigate the effect of noise on frequency-domain heterodyne and/or homodyne measurements of intensity-modulated beams propagating through diffusive media, such as a photon density wave. We assumed that the attenuated amplitude and delayed phase are estimated by taking the Fourier transform of the n...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.1.016010
更新日期:2012-01-01 00:00:00
abstract::A reflectance volume holographic imaging (VHI) endoscope has been designed for simultaneous in vivo imaging of surface and subsurface tissue structures. Prior utilization of VHI systems has been limited to ex vivo tissue imaging. The VHI system presented in this work is designed for laparoscopic use. It consists of a ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.5.056010
更新日期:2017-05-01 00:00:00
abstract::Autofluorescence-based imaging techniques have become very important in the ophthalmological field. Being noninvasive and very sensitive, they are broadly used in clinical routines. Conventional autofluorescence intensity imaging is largely influenced by the strong fluorescence of lipofuscin, a fluorophore that can be...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.JBO.23.9.091415
更新日期:2018-09-01 00:00:00
abstract::In the last 25 years, optical coherence tomography (OCT) has advanced to be one of the most innovative and most successful translational optical imaging techniques, achieving substantial economic impact as well as clinical acceptance. This is largely owing to the resolution improvements by a factor of 10 to the submic...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.JBO.19.7.071412
更新日期:2014-01-01 00:00:00
abstract::Discrete form sensitivity functions are derived for a number of measurement types employed in autocorrelation-based ultrasound-modulated optical tomography. The Jacobian for a particular problem is constructed from the set of such sensitivity functions arising from a raster scan of a focused ultrasound field through a...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.12.126020
更新日期:2013-12-01 00:00:00
abstract::In the search for improved imaging modalities for detection and diagnosis of breast cancer, a high negative prediction value is also important. Photoacoustic (optoacoustic) imaging is a relatively new technique that has high potential for visualizing breast malignancies, but little is known about the photoacoustic app...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.12.126009
更新日期:2013-12-01 00:00:00
abstract::A hyperspectral image projector (HIP) based on liquid crystal on silicon spatial light modulators is explained and demonstrated to generate data cubes. The HIP-constructed data cubes are three-dimensional images of the spatial distribution of spectrally resolved abundances of intracellular light-absorbing oxyhemoglobi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,收录出版
doi:10.1117/1.JBO.20.11.111215
更新日期:2015-01-01 00:00:00
abstract::Three major analytical tools in imaging science are summarized and demonstrated relative to optical imaging in vivo. Standard resolution testing is optimal when infinite contrast is used and hardware evaluation is the goal. However, deep tissue imaging of absorption or fluorescent contrast agents in vivo often present...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.2209908
更新日期:2006-05-01 00:00:00
abstract::Cellularity of the fibrous caps of coronary atheromas, manifested by the infiltration of macrophages (average size, 20 to 30 microm), is thought to weaken the structural integrity of the cap and predispose plaques to rupture. Therefore, an imaging technology capable of identifying macrophages within fibroatheroma caps...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.3290810
更新日期:2010-01-01 00:00:00
abstract::A modified spatial-temporal speckle correlation technique for operational assessment of structural changes in muscle tissues after slaughtering is considered. Coefficient of biological activity as a quantitative indicator of structural changes of biochemical processes in biological tissues is proposed. The experimenta...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.9.095006
更新日期:2015-09-01 00:00:00
abstract::The specificity of molecular and functional photoacoustic (PA) images depends on the accuracy of the photoacoustic absorption spectroscopy. The PA signal is proportional to the product of the optical absorption coefficient and local light fluence; quantitative PA measurements of the optical absorption coefficient ther...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3626212
更新日期:2011-09-01 00:00:00
abstract::Laser speckle contrast imaging is a technique used for imaging blood flow without scanning. Though several studies have attempted to combine spatial and temporal statistics of laser speckle images for reducing image noise as well as preserving acceptable spatiotemporal resolution, the statistical accuracy of these spa...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3290804
更新日期:2010-01-01 00:00:00
abstract::We present a novel confocal microscope that has dual-axis architecture and biaxial postobjective scanning for the collection of fluorescence images from biological specimens. This design uses two low-numerical-aperture lenses to achieve high axial resolution and long working distance, and the scanning mirror located d...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1760760
更新日期:2004-07-01 00:00:00
abstract::Near-infrared confocal microendoscopy is a promising technique for deep in vivo imaging of tissues and can generate high-resolution cross-sectional images at the micron-scale. We demonstrate the use of a dual-axis confocal (DAC) near-infrared fluorescence microendoscope with a 5.5-mm outer diameter for obtaining clini...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.2.021102
更新日期:2012-02-01 00:00:00
abstract::Alzheimer's disease (AD) is a neurodegenerative disorder that leads to a decline in cognitive and intellectual abilities and an irreversible mental deterioration. Based on multidisciplinary AD research, the most universally accepted hypotheses on AD pathogenesis are the intracerebral aggregate formation of beta-amyloi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.5.051205
更新日期:2014-05-01 00:00:00
abstract::Gap junction-mediated intercellular communication influences a variety of cellular activities. In tendons, gap junctions modulate collagen production, are involved in strain-induced cell death, and are involved in the response to mechanical stimulation. The aim of the present study was to investigate gap junction-medi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.1.015001
更新日期:2014-01-01 00:00:00
abstract::Lumpectomy coupled with radiation therapy and/or chemotherapy is commonly used to treat breast cancer patients. We are developing an enhanced thermal IR imaging technique that has the potential to provide real-time imaging to guide tissue excision during a lumpectomy by delineating tumor margins. This enhanced thermal...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.11.116003
更新日期:2015-11-01 00:00:00
abstract::In laser retinal photocoagulation, short (<20 ms) pulses have been found to reduce thermal damage to the inner retina, decrease treatment time, and minimize pain. However, the safe therapeutic window (defined as the ratio of power for producing a rupture to that of mild coagulation) decreases with shorter exposures. T...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3130282
更新日期:2009-05-01 00:00:00
abstract::The capability of optical coherence tomography (OCT) to perform "optical biopsy" of tissues within a depth range of 1 to 2 mm with micron-scale resolution in real time makes it a promising biomedical imaging modality for dermatologic applications. Three high-speed, spectrometer-based frequency-domain OCT systems opera...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3400665
更新日期:2010-03-01 00:00:00