Abstract:
:A new imaging approach, structured light scatteroscopy (SLS), is demonstrated, which offers rapid wide-field imaging of microscopic morphological variations in bulk tissue surfaces. Elastic scattering of light offers exquisite sensitivity to ultrastructural changes at multiple size scales ranging from nanometers to millimeters, but in bulk tissues the confounding effects of molecular absorption and strong multiple scattering of light often lead to a dramatic reduction in scatter contrast and specificity. It is demonstrated that the SLS using structured high spatial frequency illumination and detection to probe the tissue achieves direct, absorption-independent, high-resolution maps of the scattering response. The scattering response is observed to be dependent on both the wavelength and spatial frequency of choice, indicating a potential for multiscale probing of ultrastructural changes in superficial tissue layers. This methodology can be easily applied in most wide-field imaging systems.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Krishnaswamy V,Elliott JT,McClatchy DM 3rd,Barth RJ Jr,Wells WA,Pogue BW,Paulsen KDdoi
10.1117/1.JBO.19.7.070504subject
Has Abstractpub_date
2014-01-01 00:00:00pages
070504issue
7eissn
1083-3668issn
1560-2281pii
1891726journal_volume
19pub_type
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