Abstract:
:A new, simple, high-speed, and hardware-only integration-based fluorescence-lifetime-sensing algorithm using a center-of-mass method (CMM) is proposed to implement lifetime calculations, and its signal-to-noise-ratio based on statistics theory is also deduced. Compared to the commonly used iterative least-squares method or the maximum-likelihood-estimation-based, general purpose fluorescence lifetime imaging microscopy (FLIM) analysis software, the proposed hardware lifetime calculation algorithm with CMM offers direct calculation of fluorescence lifetime based on the collected photon counts and timing information provided by in-pixel circuitry and therefore delivers faster analysis for real-time applications, such as clinical diagnosis. A real-time hardware implementation of this CMM FLIM algorithm suitable for a single-photon avalanche diode array in CMOS imaging technology is now proposed for implementation on field-programmable gate array. The performance of the proposed methods has been tested on Fluorescein, Coumarin 6, and 1,8-anilinonaphthalenesulfonate in water/methanol mixture.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Li DU,Rae B,Andrews R,Arlt J,Henderson Rdoi
10.1117/1.3309737subject
Has Abstractpub_date
2010-01-01 00:00:00pages
017006issue
1eissn
1083-3668issn
1560-2281journal_volume
15pub_type
杂志文章abstract:SIGNIFICANCE:Despite the importance of the cell membrane in regulation of drug activity, the influence of drug treatments on its physical properties is still poorly understood. The combination of fluorescence lifetime imaging microscopy (FLIM) with specific viscosity-sensitive fluorescent molecular rotors allows the qu...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.25.12.126004
更新日期:2020-12-01 00:00:00
abstract::As molecular imaging moves towards lower detection limits, the elimination of endogenous background signals becomes imperative. We present a facile background-suppression technique that specifically segregates the signal from surface-enhanced Raman scattering (SERS)-active nanoparticles (NPs) from the tissue autofluor...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.7.076017
更新日期:2012-07-01 00:00:00
abstract::An evolving area of biomedical research is related to the creation of implantable units that provide various possibilities for imaging, measurement, and the monitoring of a wide range of diseases and intrabody phototherapy. The units can be autonomic or built-in in some kind of clinically applicable implants. Because ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.10.108001
更新日期:2014-01-01 00:00:00
abstract::The ability to noninvasively image embryonic vascular anatomy in mouse models is an important requirement for characterizing the development of the normal cardiovascular system and malformations in the heart and vascular supply. Photoacoustic imaging, which can provide high resolution non invasive images of the vascul...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.6.061220
更新日期:2012-06-01 00:00:00
abstract::Ex vivo human skin has been used extensively for cosmeceutical and drug delivery studies, transplantable skin allografts, or skin flaps. However, it has a half-life of a few days due to ischemic necrosis. Traditional methods of assessing viability can be time-consuming and provide limited metabolic information. Using ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3466580
更新日期:2010-07-01 00:00:00
abstract::In the present study, we evaluated the applicability of ex vivo photoacoustic imaging (PAI) on small animal organs. We used photoacoustic tomography (PAT) to visualize infarcted areas within murine hearts and compared these data to other imaging techniques [magnetic resonance imaging (MRI), micro-computed tomography] ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3556720
更新日期:2011-03-01 00:00:00
abstract::As the most abundant cell type in the central nervous system, astrocyte has been one of main research topics in neuroscience. Although various tools have been developed, at present, there is no tool that allows noninvasive activation of astrocyte in vivo without genetic or pharmacological perturbation. Here we report ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3600774
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abstract::This study aimed to evaluate the concept of using high-resolution optical coherence tomography (OCT) imaging to rapidly assess surgical specimens and determine if cancer positive margins were left behind in the surgical bed. A mouse model of breast cancer was used in this study. Surgical specimens from 30 animals were...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.5.056001
更新日期:2014-05-01 00:00:00
abstract::Selective retina treatment (SRT) is a novel short pulsed laser therapy of several retinal diseases associated with a decreased metabolism at the retinal pigment epithelium (RPE). The range of laser pulse energies is small, in which the desired selective RPE disintegration is achieved without adverse effects to the neu...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2339815
更新日期:2006-07-01 00:00:00
abstract::Diffusion of two model drugs-benzyl nicotinate and ibuprofen-and the plasma macromolecule albumin across atherosclerotic rabbit aorta was studied ex vivo by attenuated total reflection-Fourier transform infrared (ATR-FTIR) imaging. Solutions of these molecules were applied to the endothelial surface of histological se...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3174395
更新日期:2009-07-01 00:00:00
abstract::We present a study using a method able to assess tissue oxygenation, taking into account the absorption and the level of scattering in myocardial tissue using a calibrated fiber optic probe. With this method, interindividual comparisons of oxygenation can be made despite varying tissue optical properties during corona...
journal_title:Journal of biomedical optics
pub_type: 临床试验,杂志文章
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更新日期:2008-09-01 00:00:00
abstract::Amblyopia ("lazy eye") is a major public health problem, caused by misalignment of the eyes (strabismus) or defocus. If detected early in childhood, there is an excellent response to therapy, yet most children are detected too late to be treated effectively. Commercially available vision screening devices that test fo...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.6.067004
更新日期:2014-06-01 00:00:00
abstract::Suture ligation of blood vessels during surgery can be time-consuming and skill-intensive. Energy-based, electrosurgical, and ultrasonic devices have recently replaced the use of sutures and mechanical clips (which leave foreign objects in the body) for many surgical procedures, providing rapid hemostasis during surge...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.5.058001
更新日期:2013-05-01 00:00:00
abstract::Color Doppler optical coherence tomography (CDOCT) is a functional extension of optical coherence tomography (OCT) that can image flow in turbid media. We have developed a CDOCT system capable of imaging flow in real time. Doppler processing of the analog signal is accomplished in hardware in the time domain using a n...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1428291
更新日期:2002-01-01 00:00:00
abstract::We present the application of a microfluidic optical cell stretcher to measure the elasticity of malaria-infected red blood cells. The measurements confirm an increase in host cell rigidity during the maturation of the parasite Plasmodium falciparum. The device combines the selectivity and sensitivity of single-cell e...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3458919
更新日期:2010-05-01 00:00:00
abstract::In order to monitor dynamic physiological events in near-real time, a variety of photoacoustic computed tomography (PACT) systems have been developed that can rapidly acquire data. Previously reported studies of dynamic PACT have employed conventional static methods to reconstruct a temporally ordered sequence of imag...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.5.056007
更新日期:2014-05-01 00:00:00
abstract::We fabricate scaffolds with a fine linear grating (periodicity of 1 to 8 microm and height of 1 microm) on a glass substrate for controlling cardiomyocyte orientation. The fabrication is done by the solidification of a liquid photopolymerizable material using two laser beam interference. As the photopolymerizable mate...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2186042
更新日期:2006-03-01 00:00:00
abstract::Real-time monitoring of functional tissue parameters, such as local blood oxygenation, based on optical imaging could provide groundbreaking advances in the diagnosis and interventional therapy of various diseases. Although photoacoustic (PA) imaging is a modality with great potential to measure optical absorption dee...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.23.5.056008
更新日期:2018-05-01 00:00:00
abstract::Confocal micro-Raman spectroscopy--a valuable analytical tool in biological and medical field of research-allows probing molecular vibrations of samples without external labels or extensive preparation. We employ confocal micro-Raman spectroscopy to characterize renal tumors and normal tissue. Results show that Raman ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.3.031103
更新日期:2013-03-01 00:00:00
abstract::Since 1978 in vitro fertilization (IVF) procedures have resulted in the birth of over 3 million babies. Yet in 2005, IVF procedures had a live birth rate of only 34%, with 32% of these births resulting in multiple pregnancies. These multiple pregnancies were directly attributed to the transfer of multiple embryos to i...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
abstract::Under ultraviolet and visible light excitation, melanin is essentially a nonfluorescent substance. This work reports our study on near-infrared (NIR) fluorescence properties of melanins, and explores potential applications of NIR fluorescence techniques for evaluating skin disorders involving melanin. The NIR fluoresc...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2006-05-01 00:00:00
abstract::The efficacy of existing therapies and the discovery of innovative treatments for central nervous system (CNS) diseases have been limited by the lack of appropriate methods to investigate complex molecular processes at the synaptic level. To improve our capability to investigate complex mechanisms of synaptic signalin...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2016-04-30 00:00:00
abstract:UNLABELLED:Temperature changes caused by laser irradiation can promote damage to the surrounding dental tissues. In this study, we evaluated the temperature changes of recently extracted human mandibular incisors during intracanal irradiation with an 810-nm diode laser at different settings. Fifty mandibular incisors w...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2012-01-01 00:00:00
abstract::We investigated the effect of thiazone, a widely used penetration enhancer, on in vitro porcine skin and muscle tissue by single-integrating sphere technique during optical clearing (OC) treatment. The results showed that thiazone induced an increase on the total transmittance of skin which led to a reduction in that ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2016-10-01 00:00:00
abstract::We propose an offset-sparsity decomposition method for the enhancement of a color microscopic image of a stained specimen. The method decomposes vectorized spectral images into offset terms and sparse terms. A sparse term represents an enhanced image, and an offset term represents a "shadow." The related optimization ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.7.076012
更新日期:2015-07-01 00:00:00
abstract::A highly efficient method based on Maxwell's theory was developed, which enables the calculation of the scanning of a focused beam through scattering media. Maxwell's equations were numerically solved in two dimensions using finite difference time domain simulations. The modeling of the focused beam was achieved by ap...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2014-07-01 00:00:00
abstract::Quantitative polarized light microscopy (qPLM) is a popular tool for the investigation of birefringent architectures in biological tissues. Collagen, the most abundant protein in mammals, is such a birefringent material. Interpretation of results of qPLM in terms of collagen network architecture and anisotropy is chal...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3241986
更新日期:2009-09-01 00:00:00
abstract::A transgenic mouse model with a Clim [co-factor of LIM (a combination of first letters of Lin-11 (C. elegans), ISL1 (rat), and Mec-3 (C. elegans) gene names) domain proteins] gene partially blocked in the epithelial compartment of its tissues is used to establish the sensitivity of intrinsic reflectance nonlinear opti...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
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abstract::We report an accurate optical differentiation technique between healthy and malaria-infected erythrocytes by quasi-simultaneous measurements of transmittance, reflectance, and scattering properties of unstained blood smears using a multispectral and multimode light-emitting diode microscope. We propose a technique for...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.3.036002
更新日期:2013-03-01 00:00:00
abstract::An approach to an established technique that is potentially applicable for a more comprehensive understanding of the electrical properties of red blood cells (RBCs) is presented. Using a high-intensity gradient laser trap, RBCs can be singly trapped and consequentially ionized. The subsequent dynamics of the ionized c...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2018-05-01 00:00:00