Filter-based method for background removal in high-sensitivity wide-field-surface-enhanced Raman scattering imaging in vivo.

Abstract:

:As molecular imaging moves towards lower detection limits, the elimination of endogenous background signals becomes imperative. We present a facile background-suppression technique that specifically segregates the signal from surface-enhanced Raman scattering (SERS)-active nanoparticles (NPs) from the tissue autofluorescence background in vivo. SERS NPs have extremely narrow spectral peaks that do not overlap significantly with endogenous Raman signals. This can be exploited, using specific narrow-band filters, to image picomolar (pM) concentrations of NPs against a broad tissue autofluorescence background in wide-field mode, with short integration times that compare favorably with point-by-point mapping typically used in SERS imaging. This advance will facilitate the potential applications of SERS NPs as contrast agents in wide-field multiplexed biomarker-targeted imaging in vivo.

journal_name

J Biomed Opt

authors

Mallia RJ,McVeigh PZ,Veilleux I,Wilson BC

doi

10.1117/1.JBO.17.7.076017

subject

Has Abstract

pub_date

2012-07-01 00:00:00

pages

076017

issue

7

eissn

1083-3668

issn

1560-2281

journal_volume

17

pub_type

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