Abstract:
:As molecular imaging moves towards lower detection limits, the elimination of endogenous background signals becomes imperative. We present a facile background-suppression technique that specifically segregates the signal from surface-enhanced Raman scattering (SERS)-active nanoparticles (NPs) from the tissue autofluorescence background in vivo. SERS NPs have extremely narrow spectral peaks that do not overlap significantly with endogenous Raman signals. This can be exploited, using specific narrow-band filters, to image picomolar (pM) concentrations of NPs against a broad tissue autofluorescence background in wide-field mode, with short integration times that compare favorably with point-by-point mapping typically used in SERS imaging. This advance will facilitate the potential applications of SERS NPs as contrast agents in wide-field multiplexed biomarker-targeted imaging in vivo.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Mallia RJ,McVeigh PZ,Veilleux I,Wilson BCdoi
10.1117/1.JBO.17.7.076017subject
Has Abstractpub_date
2012-07-01 00:00:00pages
076017issue
7eissn
1083-3668issn
1560-2281journal_volume
17pub_type
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