Abstract:
:An approach to an established technique that is potentially applicable for a more comprehensive understanding of the electrical properties of red blood cells (RBCs) is presented. Using a high-intensity gradient laser trap, RBCs can be singly trapped and consequentially ionized. The subsequent dynamics of the ionized cell allows one to calculate the charge developed and the ionization energy (IE) through a Newtonian-based analysis. RBCs with two different hemoglobin (Hb) types were ionized. The first sample was identified as carrying Hb HbAA (normal Hb) and the second one was identified as carrying HbAC (HbC trait). By analyzing the charge developed on each cell and several other related factors, we were able to discern a difference between the main Hb types contained within the individual RBC, independent of cell size. A relationship between the charge developed and the IE of the cell was also established based on the electrical properties of RBCs. Thus, we present this laser trapping technique as a study of the electrical properties of RBCs and as possible biomedical tool to be used for the differentiation of Hb types.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Kelley M,Cooper J,Devito D,Mushi R,Aguinaga MDP,Erenso DBdoi
10.1117/1.JBO.23.5.055005subject
Has Abstractpub_date
2018-05-01 00:00:00pages
1-10issue
5eissn
1083-3668issn
1560-2281pii
JBO-180110RRjournal_volume
23pub_type
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