Abstract:
:Intraductal carcinoma is a precancerous lesion of the breast and the immediate precursor of invasive ductal carcinoma. Multiphoton microscopy (MPM) was used to monitor the progression from intraductal carcinoma to invasive ductal carcinoma, which can improve early detection of precursor lesions and halt progression to invasive neoplastic disease. It was found that MPM has the capability to reveal the qualitative changes in features of cells, structure of basement membranes, and architecture of collagens during the development from intraductal carcinoma to invasive ductal carcinoma, as well as the quantitative alterations in nuclear area, circle length of basement membrane, and collagen density. Combined with intra-fiberoptic ductoscopy or transdermal biopsy needle, MPM has the potential to provide immediate histological diagnosis of tumor progression in the field of breast carcinoma.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Wu Y,Fu F,Lian Y,Nie Y,Zhuo S,Wang C,Chen Jdoi
10.1117/1.JBO.20.9.096007subject
Has Abstractpub_date
2015-09-01 00:00:00pages
096007issue
9eissn
1083-3668issn
1560-2281pii
2442345journal_volume
20pub_type
杂志文章abstract::Fourier transform infrared (FTIR) spectroscopic imaging has been used to probe the biochemical composition of human renal tumor tissue and adjacent normal tissue. Freshly resected renal tumor tissue from surgery was prepared as a thin cryosection and examined by FTIR spectroscopic imaging. Tissue types could be discri...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3622292
更新日期:2011-09-01 00:00:00
abstract::We describe the use of a biochip based on complementary metal oxide semiconductor (CMOS) technology for detection of specific genetic sequences using molecular beacons (MB) immobilized on solid surfaces as probes. The applicability of this miniature detection system for screening for the BRCA1 gene is evaluated using ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1691025
更新日期:2004-05-01 00:00:00
abstract::The measurement of changes in blood volume in tissue is important for monitoring the effects of a wide range of therapeutic interventions, from radiation therapy to skin-flap transplants. Many systems available for purchase are either expensive or difficult to use, limiting their utility in the clinical setting. A low...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.10.105005
更新日期:2014-01-01 00:00:00
abstract::Pulse-modulated second harmonic imaging microscopes (PM-SHIMs) exhibit improved signal-to-noise ratio (SNR) over conventional SHIMs on sensitive imaging and quantification of weak collagen signals inside tissues. We quantify the spatial distribution of sparse collagen inside a xenograft model of human acute myeloid le...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3497565
更新日期:2010-09-01 00:00:00
abstract::Measuring the elasticity distribution inside the human body is of great interest because elastic abnormalities can serve as indicators of several diseases. We present a method for mapping elasticity inside soft tissues by imaging surface acoustic waves (SAWs) with digital holographic interferometry. With this method, ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3646211
更新日期:2011-11-01 00:00:00
abstract::Previous studies suggest that altered corneal temperature may be a feature of schizophrenia, but the association between major depressive disorder (MDD) and corneal temperature has yet to be assessed. The aim of this study is to investigate whether eye temperature is different among MDD patients than among healthy ind...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.2.026001
更新日期:2016-02-01 00:00:00
abstract::Sinus blockages are a common reason for physician visits, affecting one out of seven people in the United States, and often require medical treatment. Diagnosis in the primary care setting is challenging because symptom criteria (via detailed clinical history) plus objective imaging [computed tomography (CT) or endosc...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.3.035004
更新日期:2015-03-01 00:00:00
abstract::We investigate the statistical polarization parameters of biological tissue histological section images with different morphological structure. First we outline the results of polarization coordinate mapping and analysis of the statistics of the first to fourth orders of biological tissue image polarization azimuth an...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2148251
更新日期:2005-11-01 00:00:00
abstract::Ultrasmall scale implants have recently attracted focus as valuable tools for monitoring both acute and chronic diseases. Semiconductor optical technologies are the key to miniaturizing these devices to the long-sought sub-mm scale, which will enable long-term use of these devices for medical applications. This can al...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.9.095012
更新日期:2015-09-01 00:00:00
abstract::As the most abundant cell type in the central nervous system, astrocyte has been one of main research topics in neuroscience. Although various tools have been developed, at present, there is no tool that allows noninvasive activation of astrocyte in vivo without genetic or pharmacological perturbation. Here we report ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3600774
更新日期:2011-07-01 00:00:00
abstract::A reflectance volume holographic imaging (VHI) endoscope has been designed for simultaneous in vivo imaging of surface and subsurface tissue structures. Prior utilization of VHI systems has been limited to ex vivo tissue imaging. The VHI system presented in this work is designed for laparoscopic use. It consists of a ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.5.056010
更新日期:2017-05-01 00:00:00
abstract::The nEUROPt protocol is one of two new protocols developed within the European project nEUROPt to characterize the performances of time-domain systems for optical imaging of the brain. It was applied in joint measurement campaigns to compare the various instruments and to assess the impact of technical improvements. T...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.8.086012
更新日期:2014-08-01 00:00:00
abstract::We present systematic characterization of a photoacoustic imaging system optimized for rapid, high-resolution tomographic imaging of small animals. The system is based on a 128-element ultrasonic transducer array with a 5-MHz center frequency and 80% bandwidth shaped to a quarter circle of 25 mm radius. A 16-channel d...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2907157
更新日期:2008-03-01 00:00:00
abstract::Intercellular heterogeneity is a key factor in a variety of core cellular processes including proliferation, stimulus response, carcinogenesis, and drug resistance. However, cell-to-cell variability studies at the single-cell level have been hampered by the lack of enabling experimental techniques. We present a measur...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.3.037008
更新日期:2012-03-01 00:00:00
abstract::Peripheral nerve injury in vivo promotes a regenerative growth in vitro characterized by an improved neurite regrowth. Knowledge of the conditioning injury effects on both morphology and mechanical properties of live sensory neurons could be instrumental to understand the cellular and molecular mechanisms leading to t...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.10.106014
更新日期:2013-10-01 00:00:00
abstract::Fluorescence spectroscopy is a promising technology for detection of epithelial precancers and cancers. While age and menopausal status influence measurements in the cervix, other variables do not significantly affect the diagnosis. In this study we examine probe pressure as a variable. A fiber optic probe to measure ...
journal_title:Journal of biomedical optics
pub_type: 临床试验,杂志文章
doi:10.1117/1.1695562
更新日期:2004-05-01 00:00:00
abstract::In this work experimental tissue phantoms and numerical models were developed to estimate photon migration through the fetal head in utero. The tissue phantoms incorporate a fetal head within an amniotic fluid sac surrounded by a maternal tissue layer. A continuous wave, dual-wavelength (lambda = 760 and 850 nm) spect...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.429983
更新日期:2000-04-01 00:00:00
abstract::Blood smear analysis has remained a crucial diagnostic tool for pathologists despite the advent of automatic analyzers such as flow cytometers and impedance counters. Though these current methods have proven to be indispensible tools for physicians and researchers alike, they provide limited information on the detaile...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3369965
更新日期:2010-03-01 00:00:00
abstract::Optical coherence microscopy (OCM) combines confocal microscopy and optical coherence tomography (OCT) to improve imaging depth and contrast, enabling cellular imaging in human tissues. We aim to investigate OCM for ex vivo imaging of upper and lower gastrointestinal tract tissues, to establish correlations between OC...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3322704
更新日期:2010-01-01 00:00:00
abstract::Higher-order optical errors of the human eye are often responsible for reduced visual acuity in spite of an optimal spherical or cylindrical refraction. These optical aberrations are of natural origin or can result from operations in the eye that involve optical structures. The ocular aberrometer presented is based on...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1344589
更新日期:2001-04-01 00:00:00
abstract::An integrated miniature multi-modal microscope (4M device) for microendoscopy was built and tested. Imaging performance is evaluated and imaging results are presented for both fluorescence and reflectance samples. Images of biological samples show successful imaging of both thin layers of fixed cells prepared on a sli...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2978060
更新日期:2008-09-01 00:00:00
abstract::Two-photon excitation microscopy is one of the key techniques used to observe three-dimensional (3-D) structures in biological samples. We utilized a visible-wavelength laser beam for two-photon excitation in a multifocus confocal scanning system to improve the spatial resolution and image contrast in 3-D live-cell im...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.25.1.014502
更新日期:2019-11-01 00:00:00
abstract::We assess the properties of contact pressure applied by manually operated fiber-optic probes as a function of the operator, probe contact area, and sample stiffness. First, the mechanical properties of human skin sites with different skin structures, thicknesses, and underlying tissues were studied by in vivo indentat...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.12.127002
更新日期:2015-01-01 00:00:00
abstract::We describe the protocol for an inexpensive and nondestructive optical reflectance assay for the measurement of biofilm formation. Reflectance data are obtained using an Ocean Optics (Dunedin, Florida) USB 2000 spectrometer with a polychromatic light source. A fiber optic cable is used both for illumination and collec...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1953347
更新日期:2005-07-01 00:00:00
abstract::Common methods to characterize treatment efficacy based on morphological imaging may misrepresent outcomes and exclude effective therapies. Using a three-dimensional model of ovarian cancer, two functional treatment response metrics are used to evaluate photodynamic therapy (PDT) efficacy: total volume, calculated fro...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.9.098004
更新日期:2013-09-01 00:00:00
abstract::We investigate optoelectronic properties of integrated structures comprising semiconductor light-emitting materials for optical probes of microscopic biological systems. Compound semiconductors are nearly ideal light emitters for probing cells and other microorganisms because of their spectral match to the transparenc...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1506931
更新日期:2002-10-01 00:00:00
abstract::High-speed (video-rate) fluorescence lifetime imaging (FLIM) through a flexible endoscope is reported based on gated optical image intensifier technology. The optimization and potential application of FLIM to tissue autofluorescence for clinical applications are discussed. ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2102807
更新日期:2005-09-01 00:00:00
abstract::We demonstrate the in vivo assessment of human scars by parametric imaging of birefringence using polarization-sensitive optical coherence tomography (PS-OCT). Such in vivo assessment is subject to artifacts in the detected birefringence caused by scattering from blood vessels. To reduce these artifacts, we preprocess...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.12.126014
更新日期:2014-12-01 00:00:00
abstract::A new imaging approach, structured light scatteroscopy (SLS), is demonstrated, which offers rapid wide-field imaging of microscopic morphological variations in bulk tissue surfaces. Elastic scattering of light offers exquisite sensitivity to ultrastructural changes at multiple size scales ranging from nanometers to mi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.7.070504
更新日期:2014-01-01 00:00:00
abstract::We propose a rate process model for describing photochemical damage to retinal cells by short wavelength laser exposures. The rate equation for photochemical damage contains a positive rate that is temperature independent, and a negative (quenching) rate that is temperature dependent. Using the traditional Arrhenius i...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3544504
更新日期:2011-02-01 00:00:00