Abstract:
:Peripheral nerve injury in vivo promotes a regenerative growth in vitro characterized by an improved neurite regrowth. Knowledge of the conditioning injury effects on both morphology and mechanical properties of live sensory neurons could be instrumental to understand the cellular and molecular mechanisms leading to this regenerative growth. In the present study, we use differential interference contrast microscopy, fluorescence microscopy, and atomic force microscopy (AFM) to show that conditioned axotomy, induced by sciatic nerve injury, does not increase somatic size of sensory neurons from adult mice lumbar dorsal root ganglia but promotes the appearance of longer and larger neurites and growth cones. AFM on live neurons is also employed to investigate changes in morphology and membrane mechanical properties of somas of conditioned neurons following sciatic nerve injury. Mechanical analysis of the soma allows distinguishing neurons having a regenerative growth from control ones, although they show similar shapes and sizes.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Benzina O,Szabo V,Lucas O,Saab MB,Cloitre T,Scamps F,Gergely C,Martin Mdoi
10.1117/1.JBO.18.10.106014subject
Has Abstractpub_date
2013-10-01 00:00:00pages
106014issue
10eissn
1083-3668issn
1560-2281pii
1763523journal_volume
18pub_type
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