Abstract:
:We describe the use of a biochip based on complementary metal oxide semiconductor (CMOS) technology for detection of specific genetic sequences using molecular beacons (MB) immobilized on solid surfaces as probes. The applicability of this miniature detection system for screening for the BRCA1 gene is evaluated using MB probes, designed especially for the BRCA1 gene. MB probes are immobilized on a zeta-probe membrane by biotin-streptavidin immobilization. Two immobilization strategies are investigated to obtain optimal assay sensitivity. The MB is immobilized by manual spotting on zeta-probe membrane surfaces with the use of a custom-made stamping system. The detection of the BRCA1 gene using an MB probe is successfully demonstrated and expands the use of the CMOS biochip for medical applications.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Culha M,Stokes DL,Griffin GD,Vo-Dinh Tdoi
10.1117/1.1691025subject
Has Abstractpub_date
2004-05-01 00:00:00pages
439-43issue
3eissn
1083-3668issn
1560-2281journal_volume
9pub_type
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