Abstract:
:Application of a fiber optic biosensor (FOB) to the real-time investigation of the interaction kinetics between FITC-conjugated monoclonal sheep anti-human C-reactive protein (CRP) antibody and CRP isoforms on the surface of optical fiber is described. Recently, both the native pentameric CRP (pCRP), an acute phase protein belonging to pentraxin family, and an isoform of pCRP, modified CRP (mCRP), have been suggested to have proinflammation effects on vascular cells in acute myocardial infarction (AMI). In current studies, we generate mCRP from pCRP, and use several methods including fluorescence spectral properties, circular dichroism, analytical ultracentrifuge, and Western blotting to demonstrate their differences in physical and chemical properties as well as the purity of pCRP and mCRP. In addition, we design and implement an FOB to study the real-time qualitative and quantitative biomolecular recognition of CRP isoforms. Specifically, the association and dissociation rate constants of the reaction between FITC-conjugated monoclonal sheep anti-human CRP antibody and the pCRP and mCRP are determined. The feasibility of our current approach to measure the association and dissociation rate constants of the reaction between tested CRP isoforms was successfully demonstrated.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Chou C,Hsu HY,Wu HT,Tseng KY,Chiou A,Yu CJ,Lee ZY,Chan TSdoi
10.1117/1.2714029subject
Has Abstractpub_date
2007-03-01 00:00:00pages
024025issue
2eissn
1083-3668issn
1560-2281journal_volume
12pub_type
杂志文章abstract::Optical measurements of metabolism are ideally acquired in vivo; however, intravital measurements are often impractical. Accurate ex vivo assessments would greatly broaden the applicability of optical measurements of metabolism. We investigate the use of live tissue culture experiments to serve as a surrogate for in v...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.11.116015
更新日期:2012-11-01 00:00:00
abstract::In the search for improved imaging modalities for detection and diagnosis of breast cancer, a high negative prediction value is also important. Photoacoustic (optoacoustic) imaging is a relatively new technique that has high potential for visualizing breast malignancies, but little is known about the photoacoustic app...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.12.126009
更新日期:2013-12-01 00:00:00
abstract::Complete and continuous imaging of microvascular networks is crucial for a wide variety of biomedical applications. Photoacoustic tomography can provide high resolution microvascular imaging using hemoglobin within red blood cells (RBCs) as an endogenic contrast agent. However, intermittent RBC flow in capillaries res...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3251044
更新日期:2009-09-01 00:00:00
abstract::We used two-photon laser scanning microscopy (TPLSM) to demonstrate for the first time its potential in studying relational details at the cellular level of atherogenesis in intact, viable mouse carotid arteries. Isolated and mounted arteries of ApoE-/-mice, aged 15 or 21 weeks (7 and 13 weeks on western diet), were i...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2965542
更新日期:2008-07-01 00:00:00
abstract::A tutorial introduction to diffuse light transport is presented. The basic analytic equations of time-resolved, steady-state and modulated light transport are introduced. The perturbation method for handling slight heterogeneities in optical properties is outlined. The treatment of boundary conditions such as an air/t...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2967535
更新日期:2008-07-01 00:00:00
abstract::An algorithm was developed to obtain depth-resolved local optical axis in birefringent samples by using conventional polarization-sensitive optical coherence tomography (PSOCT) that uses a single circularly polarized incident light. The round-trip sample Jones matrices were first constructed from the cumulative PSOCT ...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.JBO.17.11.110501
更新日期:2012-11-01 00:00:00
abstract::Cortical spreading depression (CSD) is a pronounced depolarization of neurons and glia that spreads slowly across the cortex followed by a period of depressed electrophysiological activity. The vascular changes associated with CSD are a large transient increase in blood flow followed by a prolonged decrease lasting gr...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1852556
更新日期:2005-01-01 00:00:00
abstract::Differential phase optical coherence tomography (DPOCT) is introduced to measure optical path length changes in response to pulsed laser irradiation (585 nm). An analytical equation that includes thermoelastic surface displacement and thermorefractive index change is derived to predict optical path length change in re...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2236289
更新日期:2006-07-01 00:00:00
abstract:SIGNIFICANCE:Optical coherence tomography (OCT) has proven useful for detecting various oral maxillofacial abnormalities. To apply it to clinical applications including biopsy guidance and routine screening, a handheld imaging probe is indispensable. OCT probes reported for oral maxillofacial imaging were either based ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.25.4.046003
更新日期:2020-04-01 00:00:00
abstract::Resin embedding is widely used and facilitates microscopic imaging of biological tissues. In contrast, quenching of fluorescence during embedding process hinders the application of resin embedding for imaging of fluorescence-labeled samples. For samples expressing fluorescent proteins, it has been demonstrated that th...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.23.2.020501
更新日期:2018-02-01 00:00:00
abstract::UV-induced collagen cross-linking is a promising treatment for keratoconus that stiffens corneal tissue and prevents further degeneration. Since keratoconus is generally localized, the efficacy of collagen cross-linking (CXL) treatments could be improved by stiffening only the weakened parts of the cornea. Here, we de...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.9.091504
更新日期:2017-09-01 00:00:00
abstract::Raman spectroscopy on single, living epithelial cells captured in a laser trap is shown to have diagnostic power over colorectal cancer. This new single-cell technology comprises three major components: primary culture processing of human tissue samples to produce single-cell suspensions, Raman detection on singly tra...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2748060
更新日期:2007-05-01 00:00:00
abstract::Laser welding has the potential to become an effective method for wound closure and healing without sutures. Closure of skin incisions by laser welding with a combination of two near-infrared lasers (980 and 1064 nm), was performed for the first time in this study. One centimeter long, full-thickness incisions were ma...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3552648
更新日期:2011-03-01 00:00:00
abstract::A simultaneous multispectral fluorescence imaging system incorporating multiplexed volume holographic grating (VHG) is developed to acquire multispectral images of an object in one shot. With the multiplexed VHG, the imaging system can provide the distribution and spectral characteristics of multiple fluorophores in t...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.6.060502
更新日期:2016-06-01 00:00:00
abstract::Intraoperative fluorescence molecular imaging based on targeted fluorescence agents is an emerging approach to improve surgical and endoscopic imaging and guidance. Short exposure times per frame and implementation at video rates are necessary to provide continuous feedback to the physician and avoid motion artifacts....
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.4.046012
更新日期:2014-04-01 00:00:00
abstract::An evolving area of biomedical research is related to the creation of implantable units that provide various possibilities for imaging, measurement, and the monitoring of a wide range of diseases and intrabody phototherapy. The units can be autonomic or built-in in some kind of clinically applicable implants. Because ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.10.108001
更新日期:2014-01-01 00:00:00
abstract::Collagen fibers can be imaged with second harmonic generation (SHG) and are associated with efficient tumor cell locomotion. Preferential locomotion along these fibers correlates with a more aggressively metastatic phenotype, and changes in SHG emission properties accompany changes in metastatic outcome. We therefore ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.8.086003
更新日期:2013-08-01 00:00:00
abstract::This paper explains the term low-coherence interferometry, reviews the early development of optical low-coherence reflectometry, and shows some of the paths that led to the field of biomedical optics. This paper demonstrates that early technical developments in the telecommunications industry resulted in a myriad of t...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.429914
更新日期:1999-04-01 00:00:00
abstract::This study introduces a single-channel optical video stereomicroscope based on a transparent rotating deflector (TRD) for high-resolution and high-magnification stereomicroscopy. The existing stereomicroscopes have some limitations, such as limited resolution and magnification, fixed optical channel, and the necessity...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.12.126001
更新日期:2016-12-01 00:00:00
abstract::Cell morphology is often used as a valuable indicator of the physical condition and general status of living cells. We demonstrate a noninvasive method for morphological characterization of adherent cells. We measure infrared reflectivity spectrum at oblique angle from living cells cultured on thin Au film, and utiliz...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.8.081409
更新日期:2012-08-01 00:00:00
abstract::Time-resolved measurement of early arriving photons through diffusive media has been shown to effectively reduce the high degree of light scatter in biological tissue. However, the experimentally achievable reduction in photon scatter and the impact of time-gated detection on instrument noise performance is not well u...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3523371
更新日期:2010-11-01 00:00:00
abstract::Selective retina treatment (SRT) is a novel short pulsed laser therapy of several retinal diseases associated with a decreased metabolism at the retinal pigment epithelium (RPE). The range of laser pulse energies is small, in which the desired selective RPE disintegration is achieved without adverse effects to the neu...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2339815
更新日期:2006-07-01 00:00:00
abstract:SIGNIFICANCE:The potential of fluorescence lifetime imaging microscopy (FLIM) is recently being recognized, especially in biological studies. However, FLIM does not directly measure the lifetimes, rather it records the fluorescence decay traces. The lifetimes and/or abundances have to be estimated from these traces dur...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.26.2.022909
更新日期:2021-01-01 00:00:00
abstract::Limited cutaneous systemic sclerosis (lcSSc) was formerly known as CREST syndrome in reference to the associated clinical features: calcinosis, Raynaud's phenomenon, esophageal dysfunction, sclerodactyly, and telangiectasias. The transforming growth factor beta has been identified as a major player in the pathogenic p...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.11.118001
更新日期:2014-01-01 00:00:00
abstract::Oral cancer is one of the most common cancers worldwide. One-fifth of the world's oral cancer subjects are from India and other South Asian countries. The present Raman mapping study was carried out to understand biochemical variations in normal and malignant oral buccal mucosa. Data were acquired using WITec alpha 30...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.12.126005
更新日期:2014-12-01 00:00:00
abstract::Vascular leakage is an important feature of various disease conditions. In vivo optical imaging provides a great opportunity for the evaluation of this phenomenon. In the present study, we focus on the development and validation of a near-infrared (NIR) imaging formula to allow reliable, cost-efficient evaluation of v...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.1.016022
更新日期:2015-01-01 00:00:00
abstract::Skin cancer diagnosis depends not only on histopathological examination but also on visual inspection before and after the excision of suspected lesion. Neoplasm is accompanied with changes in birefringence of collagen, pleomorphicity, and hyperchromatic state of epithelial nuclei. These phenomena can be measured by s...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3609003
更新日期:2011-08-01 00:00:00
abstract::Optical clearing is a well-known phenomenon. It is based on the matching of refractive indices of a bulk material and scattering particles. The same principle is also used in scattering-based optical measurements of different constituents, such as glucose. By registering changes in scattering, it is possible to evalua...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2904957
更新日期:2008-03-01 00:00:00
abstract::Different colors of visible light penetrate to varying depths in tissue due to the wavelength dependence of tissue optical absorption and elastic scattering. We exploit this to map the contour of the closest surface of a buried fluorescent object. This uses a novel algorithm based on the diffusion theory description o...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3523369
更新日期:2010-11-01 00:00:00
abstract::Optical coherence tomography (OCT) can provide new insight into disease progression and therapy by enabling nondestructive, serial imaging of in vivo cancer models. In previous studies, we have shown the utility of endoscopic OCT for identifying adenomas in the azoxymethane-treated mouse model of colorectal cancer and...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3446674
更新日期:2010-07-01 00:00:00