Abstract:
:Cell morphology is often used as a valuable indicator of the physical condition and general status of living cells. We demonstrate a noninvasive method for morphological characterization of adherent cells. We measure infrared reflectivity spectrum at oblique angle from living cells cultured on thin Au film, and utilize the unique properties of the confined infrared waves (i.e., surface plasmon and guided modes) traveling inside the cell layer. The propagation of these waves strongly depends on cell morphology and connectivity. By tracking the resonant wavelength and attenuation of the surface plasmon and guided modes we measure the kinetics of various cellular processes such as (i) cell attachment and spreading on different substrata, (ii) modulation of the outer cell membrane with chlorpromazine, and (iii) formation of intercellular junctions associated with progressive cell polarization. Our method enables monitoring of submicron variations in cell layer morphology in real-time, and in the label-free manner.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Yashunsky V,Lirtsman V,Zilbershtein A,Bein A,Schwartz B,Aroeti B,Golosovsky M,Davidov Ddoi
10.1117/1.JBO.17.8.081409subject
Has Abstractpub_date
2012-08-01 00:00:00pages
081409-1issue
8eissn
1083-3668issn
1560-2281pii
1306764journal_volume
17pub_type
杂志文章abstract::The absorption of filter substances in sunscreens, reducing the incident ultraviolet (UV) radiation, is the basis for the protecting ability of such formulations. The erythema-correlated sun protection factor (SPF), depending mainly on the intensity of the UVB radiation, is the common value to quantify the efficacy of...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3497047
更新日期:2010-09-01 00:00:00
abstract::Spatially resolved diffuse reflectance spectroscopy (srDRS) is a well-established technique for noninvasive, in vivo characterization of tissue optical properties toward diagnostic applications. srDRS has a potential for depth-resolved analysis of tissue, which is desired in various clinical situations. However, curre...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.23.11.115003
更新日期:2018-11-01 00:00:00
abstract::Fiber-guided ablation of soft tissue with pulsed holmium and thulium lasers was investigated for intraluminal incisions. A bare fiber/tissue-contact application system with a nearly tangential irradiation geometry was first used in vitro on porcine ureter tissue. The efficiency and precision of the method was analyzed...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.429865
更新日期:1998-01-01 00:00:00
abstract::Since hyperglycaemia changes the erythrocyte cell membrane fluidity and impairs cell deformity, our goal was to characterize hemoglobin and red blood cell (RBC) light refractive property changes in diabetic patients. Microscopic investigation was carried out on intact and fixed RBCs. To determine the refractive index ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1463043
更新日期:2002-04-01 00:00:00
abstract::We employ Fourier-transform Raman spectroscopy to study normal and tumoral human breast tissues, including several subtypes of cancers. We analyzed 194 Raman spectra from breast tissues that were separated into 9 groups according to their corresponding histopathological diagnosis. The assignment of the relevant Raman ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2363362
更新日期:2006-09-01 00:00:00
abstract::A primary focus of neurointensive care is monitoring the injured brain to detect harmful events that can impair cerebral blood flow (CBF), resulting in further injury. Since current noninvasive methods used in the clinic can only assess blood flow indirectly, the goal of this research is to develop an optical techniqu...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3488626
更新日期:2010-09-01 00:00:00
abstract::The ability to noninvasively image embryonic vascular anatomy in mouse models is an important requirement for characterizing the development of the normal cardiovascular system and malformations in the heart and vascular supply. Photoacoustic imaging, which can provide high resolution non invasive images of the vascul...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.6.061220
更新日期:2012-06-01 00:00:00
abstract::Diffuse optical spectroscopy (DOS) provides a powerful tool for fast and noninvasive disease diagnosis. The ability to leverage DOS to accurately quantify tissue optical parameters hinges on the model used to estimate light-tissue interaction. We describe the accuracy of a lookup table (LUT)-based inverse model for me...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.5.057001
更新日期:2012-05-01 00:00:00
abstract::Fluorescence image-guided surgery (FIGS) is an emerging technique in oncology, neurology, and cardiology. To adapt intraoperative imaging for various surgical applications, increasingly flexible and compact FIGS instruments are necessary. We present a compact, portable FIGS system and demonstrate its use in cardiovasc...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3378128
更新日期:2010-03-01 00:00:00
abstract::The composition of cartilage is predictive of its in vivo performance. Therefore, the ability to assess its primary macromolecular components, proteoglycan (PG) and collagen, is of great importance. In the current study, we hypothesized that PG content and distribution in tissue engineered cartilage could be determine...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1922329
更新日期:2005-05-01 00:00:00
abstract::Photobiological research in the last 30 yr has shown the existence of ultraweak photon emission in biological tissue, which can be detected with sophisticated photomultiplier systems. Although the emission of this ultraweak radiation, often termed biophotons, is extremely low in mammalian cells, it can be efficiently ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1899185
更新日期:2005-03-01 00:00:00
abstract::Quantitative and qualitative evaluations of structure and composition are important in monitoring development of engineered vascular tissue both in vitro and in vivo. Destructive techniques are an obstacle for performing time-lapse analyses from a single sample or animal. This study demonstrates the ability of time-re...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.8.080503
更新日期:2014-08-01 00:00:00
abstract::Limited cutaneous systemic sclerosis (lcSSc) was formerly known as CREST syndrome in reference to the associated clinical features: calcinosis, Raynaud's phenomenon, esophageal dysfunction, sclerodactyly, and telangiectasias. The transforming growth factor beta has been identified as a major player in the pathogenic p...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.11.118001
更新日期:2014-01-01 00:00:00
abstract::We theoretically investigate the effect of noise on frequency-domain heterodyne and/or homodyne measurements of intensity-modulated beams propagating through diffusive media, such as a photon density wave. We assumed that the attenuated amplitude and delayed phase are estimated by taking the Fourier transform of the n...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.1.016010
更新日期:2012-01-01 00:00:00
abstract::Optical coherence tomography (OCT) is a noninvasive imaging methodology that is able to image tissue to depths of over 1 mm. Many epithelial conditions, such as melanoma and oral cancers, require an invasive biopsy for diagnosis. A noninvasive, real-time, point of care method of imaging depth-resolved epithelial struc...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3652708
更新日期:2011-11-01 00:00:00
abstract::As limited data photoacoustic tomographic image reconstruction problem is known to be ill-posed, the iterative reconstruction methods were proven to be effective in terms of providing good quality initial pressure distribution. Often, these iterative methods require a large number of iterations to converge to a soluti...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.23.7.071204
更新日期:2018-02-01 00:00:00
abstract::In this work experimental tissue phantoms and numerical models were developed to estimate photon migration through the fetal head in utero. The tissue phantoms incorporate a fetal head within an amniotic fluid sac surrounded by a maternal tissue layer. A continuous wave, dual-wavelength (lambda = 760 and 850 nm) spect...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.429983
更新日期:2000-04-01 00:00:00
abstract::High resolution is important for sensitive detection of subtle distortions of retinal morphology at an early stage of eye diseases. We demonstrate virtually structured detection (VSD) as a feasible method to achieve in vivo super-resolution ophthalmoscopy. A line-scanning strategy was employed to achieve a super-resol...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.12.120502
更新日期:2016-12-01 00:00:00
abstract::We have been developing a Mach-Zehnder type of optical frequency domain reflectometry optical coherence tomography (OFDR-OCT) that uses discretely swept superstructure-grating distributed Bragg- reflector (SSG-DBR) lasers developed for telecommunication fields and has a 12-mm-depth range. We report images obtained wit...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2839042
更新日期:2008-01-01 00:00:00
abstract::The Nikon C1 confocal laser scanning microscope is a relatively inexpensive and user-friendly instrument. We describe a straightforward method to convert the C1 for multiphoton microscopy utilizing direct coupling of a femtosecond near-infrared laser into the scan head and fiber optic transmission of emission light to...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3139850
更新日期:2009-05-01 00:00:00
abstract::To improve surgical guidance toward prostate draining lymph nodes, we investigate the potential of intraoperative fluorescence imaging and combined pre- and intraoperative multimodality imaging approaches. Transgenic adenocarcinoma mouse prostate mice with spontaneous prostate tumors are injected intratumorally with: ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3528027
更新日期:2011-01-01 00:00:00
abstract::We successfully acquire the in vivo Raman spectrum of melanin from human skin using a rapid near-infrared (NIR) Raman spectrometer. The Raman signals of in vivo cutaneous melanin are similar to those observed from natural and synthetic eumelanins. The melanin Raman spectrum is dominated by two intense and broad peaks ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1805553
更新日期:2004-11-01 00:00:00
abstract::Vectorial models of focused beams are important to a variety of fields including microscopy, lithography, optical physics, and biomedical imaging. This has led to many models being developed, which calculate how beams of various profiles are focused both in free space and in the presence of stratified media. The major...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.23.9.090801
更新日期:2018-09-01 00:00:00
abstract::Functional near-infrared spectroscopy (fNIRS) is being increasingly applied to affective and social neuroscience research; however, the reliability of this method is still unclear. This study aimed to evaluate the test–retest reliability of the fNIRS-based prefrontal response to emotional stimuli. Twenty-six participa...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.1.016011
更新日期:2017-01-01 00:00:00
abstract::Two pronounced absorption peaks in blue and red ranges of the chlorin-based photosensitizer (PS) absorption spectrum provide additional benefits in photodynamic therapy (PDT) performance. Differing optical properties of biological tissues in these ranges allow for both dual-wavelength diagnostics and PDT performance. ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.25.6.063804
更新日期:2019-12-01 00:00:00
abstract::Lucigenin- and luminol-dependent chemiluminescence [(LC-CL) and (LM-CL)] in nondiluted human blood was studied. LM-CL was low in fresh blood and disappeared after its storage for 3 h, though the respiratory burst (RB) stimulation in blood was followed by high intensity and long-lasting LM-CL. LC-CL was high in fresh b...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.429921
更新日期:1999-01-01 00:00:00
abstract::Type I collagen is a major component of the extracellular matrix as well as many tissue engineered models. To understand changes in collagen related models over time, it is important to evaluate collagen dynamics with noninvasive techniques. Fluorescence spectroscopy provides a method to noninvasively measure endogeno...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2360516
更新日期:2006-09-01 00:00:00
abstract::The goal of this study was to determine the diagnostic capability of a multimodal spectral diagnosis (SD) for in vivo noninvasive disease diagnosis of melanoma and nonmelanoma skin cancers. We acquired reflectance, fluorescence, and Raman spectra from 137 lesions in 76 patients using custom-built optical fiber-based c...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.11.117003
更新日期:2014-01-01 00:00:00
abstract::A fully integrated trimodality fluorescence, diffuse optical, and x-ray computed tomography (FT/DOT/XCT) system for small animal imaging is reported in this work. The main purpose of this system is to obtain quantitatively accurate fluorescence concentration images using a multimodality approach. XCT offers anatomical...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3467495
更新日期:2010-07-01 00:00:00
abstract::Computer simulation is used to facilitate the design of fiber-probe geometries that enable enhanced detection of optical signals arising from specific tissue depths. Obtaining understanding of the relationship between fiber-probe design and tissue interrogation is critical when developing strategies for optical detect...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1989335
更新日期:2005-07-01 00:00:00