Abstract:
:The Nikon C1 confocal laser scanning microscope is a relatively inexpensive and user-friendly instrument. We describe a straightforward method to convert the C1 for multiphoton microscopy utilizing direct coupling of a femtosecond near-infrared laser into the scan head and fiber optic transmission of emission light to the three-channel detector box. Our adapted system can be rapidly switched between confocal and multiphoton mode, requires no modification to the original system, and uses only a few custom-made parts. The entire system, including scan mirrors and detector box, remain under the control of the user-friendly Nikon EZ-C1 software without modification.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Mancuso JJ,Larson AM,Wensel TG,Saggau Pdoi
10.1117/1.3139850subject
Has Abstractpub_date
2009-05-01 00:00:00pages
034048issue
3eissn
1083-3668issn
1560-2281journal_volume
14pub_type
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