Abstract:
:The authors' aim is to assess and quantitatively measure brain hemodynamic and morphological variations during closed-head injury (CHI) in mice using orthogonal diffuse near-infrared reflectance spectroscopy (o-DRS). CHI is a type of injury to the head that does not penetrate the skull. Usually, it is caused by mechanical blows to the head and frequently occurs in traffic accidents, falls, and assaults. Measurements of brain optical properties, namely absorption and reduced scattering coefficients in the wavelength range from 650 to 1000 nm were carried out by employing different source-detector distance and locations to provide depth sensitivity on an intact scalp over the duration of the whole experiment. Furthermore, alteration in both cortical hemodynamics and morphologic markers, i.e., scattering power and amplitude properties were derived. CHI was induced in anesthetized male mice by a weight-drop model using ∼50 g cylindrical metal falling from a height of 90 cm onto the intact scalp producing an impact of 4500 g cm. With respect to baseline, difference in brain physiological properties was observed following injury up to 1 h post-trauma. Additionally, the reduced scattering spectral shapes followed Mie scattering theory was quantified and clearly shows changes in both scattering amplitude and power from baseline indicating structural variations likely from evolving cerebral edema during CHI. We further demonstrate high correlation between scattering amplitude and scattering power, with more than 20% difference in slope in comparison to preinjury. This result indicates the possibility of using the slope also as a marker for detection of structural changes. Finally, experiments investigating brain function during the first 20 min postinjury were conducted and changes in chromophore concentrations and scattering were observed. Overall, our experiments demonstrate the potential of using the proposed technique as a valuable quantitative noninvasive tool for monitoring brain physiology following CHI injury at the bedside and/or at the field.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Abookasis D,Shochat A,Mathews MSdoi
10.1117/1.JBO.18.4.045003subject
Has Abstractpub_date
2013-04-01 00:00:00pages
045003issue
4eissn
1083-3668issn
1560-2281pii
1676102journal_volume
18pub_type
杂志文章abstract::Spatially resolved diffuse reflectance spectroscopy (srDRS) is a well-established technique for noninvasive, in vivo characterization of tissue optical properties toward diagnostic applications. srDRS has a potential for depth-resolved analysis of tissue, which is desired in various clinical situations. However, curre...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.23.11.115003
更新日期:2018-11-01 00:00:00
abstract::Optical coherence tomography (OCT) is an emergent imaging tool used for noninvasive diagnosis of skin diseases. The present meta-analysis was carried out to assess the accuracy of OCT for the diagnosis of skin cancer. We conducted a systematic literature search though EMBASE, Medline, PubMed, the Cochrane Library, and...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,meta分析
doi:10.1117/1.JBO.23.2.020902
更新日期:2018-02-01 00:00:00
abstract::The thulium fiber laser (TFL) is currently being studied as an alternative to the conventional holmium:YAG (Ho:YAG) laser for lithotripsy. The diode-pumped TFL may be electronically modulated to operate with variable parameters (e.g., pulse rate, pulse duration, and duty cycle) for studying the influence of pulse trai...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.2.028002
更新日期:2012-02-01 00:00:00
abstract::The scattering of polarized light from a two layer scattering medium is investigated using Monte Carlo simulations. First order and normalized second order moments are used to analyze the spatial properties of the emerging light in different polarization states. Linearly and circularly polarized illumination is used t...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1483316
更新日期:2002-07-01 00:00:00
abstract::Malaria parasites induce morphological, biochemical, and mechanical changes in red blood cells (RBCs). Mechanical variations are closely related to the deformability of individual RBCs. The deformation of various RBCs, including healthy and malaria-infected RBCs (iRBCs), can be directly observed through quantitative p...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.11.115003
更新日期:2015-11-01 00:00:00
abstract::SpineLab is a software tool developed for reconstructing neuronal feature skeletons from three-dimensional single- or multi-photon image stacks. These images often suffer from limited resolution and a low signal-to-noise ratio, making the extraction of morphometric information difficult. To overcome this limitation, w...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.7.076007
更新日期:2012-07-01 00:00:00
abstract::We report the design, integration, and validation of a fluorescence microscopy system for imaging of electroperturbation--the effects of nanosecond, megavolt-per-meter pulsed electric fields on biological cells and tissues. Such effects have potential applications in cancer therapy, gene regulation, and biophysical re...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2187970
更新日期:2006-03-01 00:00:00
abstract::Laser welding has the potential to become an effective method for wound closure and healing without sutures. Closure of skin incisions by laser welding with a combination of two near-infrared lasers (980 and 1064 nm), was performed for the first time in this study. One centimeter long, full-thickness incisions were ma...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3552648
更新日期:2011-03-01 00:00:00
abstract::We propose an offset-sparsity decomposition method for the enhancement of a color microscopic image of a stained specimen. The method decomposes vectorized spectral images into offset terms and sparse terms. A sparse term represents an enhanced image, and an offset term represents a "shadow." The related optimization ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.7.076012
更新日期:2015-07-01 00:00:00
abstract::Fluorescence lifetime imaging microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. Recent developments in multiphoton FLIM methods are reviewed and a novel multiphoton FLIM system using a streak...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1577574
更新日期:2003-07-01 00:00:00
abstract::An algorithm was developed to obtain depth-resolved local optical axis in birefringent samples by using conventional polarization-sensitive optical coherence tomography (PSOCT) that uses a single circularly polarized incident light. The round-trip sample Jones matrices were first constructed from the cumulative PSOCT ...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.JBO.17.11.110501
更新日期:2012-11-01 00:00:00
abstract::Cardiac architecture is inherently three-dimensional, yet most characterizations rely on two-dimensional histological slices or dissociated cells, which remove the native geometry of the heart. We previously developed a method for labeling intact heart sections without dissociation and imaging large volumes while pres...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3200939
更新日期:2009-07-01 00:00:00
abstract::Real-time medical imaging systems such as reflectance confocal microscopes and optical coherence microscopes are being tested in multiple-patient and multiple-center clinical trials. The modulation transfer function (MTF) of these systems at any given time influences the image information content and can affect the in...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2779352
更新日期:2007-09-01 00:00:00
abstract::We propose a useful method to boost the imaging speed for spectral domain optical coherence tomography (SDOCT) by multiplying a number of high-speed spectrometers used in the system with selective precise control of data-recording and data-reading phases for spectral cameras employed in each spectrometer. To demonstra...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3592492
更新日期:2011-06-01 00:00:00
abstract::A Monte Carlo algorithm is applied to simulate the measurements of a sample with glass slides sandwiched between the double integrating sphere (DIS) setup. The effects caused by various parameters, such as the sample port of integrating sphere, thicknesses, and optical properties of the sample, on light losses and opt...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2815691
更新日期:2007-11-01 00:00:00
abstract::An approach to an established technique that is potentially applicable for a more comprehensive understanding of the electrical properties of red blood cells (RBCs) is presented. Using a high-intensity gradient laser trap, RBCs can be singly trapped and consequentially ionized. The subsequent dynamics of the ionized c...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.23.5.055005
更新日期:2018-05-01 00:00:00
abstract::The objective is to characterize the impact of different ablation parameters on the thermal load during corneal refractive surgery by means of excimer laser ablation on porcine eyes. One hundred eleven ablations were performed in 105 porcine eyes. Each ablation was recorded using infrared thermography and analyzed mai...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.7.078001
更新日期:2015-07-01 00:00:00
abstract::Multiphoton microscopy has evolved into a powerful bioimaging tool in three dimensions. However, the ability to image biological specimens in-depth can be hindered by sample spherical aberration and scattering. These two phenomena can result in the degradation of image resolution and the loss of detected multiphoton s...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1924614
更新日期:2005-05-01 00:00:00
abstract::Imaging technologies working in the spatial frequency domain are becoming increasingly popular for generating wide-field maps of optical properties, enabling rapid analysis of tissue parameters. While acquisition methods have become faster and are now performing in real-time, processing methods remain slow, precluding...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.11.110501
更新日期:2016-11-01 00:00:00
abstract::This study investigates the correlation of tumor blood oxygenation and tumor pO(2) with respect to carbogen inhalation. After having refined and validated the algorithms for calculating hemoglobin concentrations, we used near-infrared spectroscopy (NIRS) to measure changes of oxygenated hemoglobin concentration (delta...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1527049
更新日期:2003-01-01 00:00:00
abstract::We examine the morphological and chemical changes in the pulp chamber dentin after using endodontic agents by scanning electron microscopy (SEM), Fourier transform Raman spectroscopy (FT-Raman), and micro energy-dispersive x-ray fluorescence spectrometry (μEDXRF). Thirty teeth were sectioned exposing the pulp chamber ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.7.075008
更新日期:2012-07-01 00:00:00
abstract::Ambulatory diffuse optical tomography (aDOT) is based on near-infrared spectroscopy (NIRS) and enables three-dimensional imaging of regional hemodynamics and oxygen consumption during a person’s normal activities. Although NIRS has been previously used for muscle assessment, it has been notably limited in terms of the...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.9.091314
更新日期:2016-09-01 00:00:00
abstract::Computer simulation is used to facilitate the design of fiber-probe geometries that enable enhanced detection of optical signals arising from specific tissue depths. Obtaining understanding of the relationship between fiber-probe design and tissue interrogation is critical when developing strategies for optical detect...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1989335
更新日期:2005-07-01 00:00:00
abstract::Microscopic visualization of islets of Langerhans under normal and diabetic conditions is essential for understanding the pathophysiology of the disease. The intrinsic opacity of pancreata, however, limits optical accessibility for high-resolution light microscopy of islets in situ. Because the standard microtome-base...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3470241
更新日期:2010-07-01 00:00:00
abstract::Mueller polarimetry is applied to study the samples of nails: natural (or reference) and irradiated to 2 Gy ionizing radiation dose. We measure the whole Mueller matrices of the samples as a function of the scattering angle at a wavelength of 632.8 nm. We apply depolarization analysis to measured Mueller matrices by c...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.7.071108
更新日期:2016-07-01 00:00:00
abstract::The atrial pacemaker complex is responsible for the initiation and early propagation of cardiac impulses. Optical coherence tomography (OCT), a nondestructive imaging modality with spatial resolutions of ∼1 to 15 μm, can be used to identify unique fiber orientation patterns in this region of the heart. Functionally ch...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.7.071309
更新日期:2012-07-01 00:00:00
abstract::In near-infrared spectroscopy, concentration changes in oxygenated and deoxygenated hemoglobin are calculated from the changes in the attenuation of the measurement light. This is done by solving a linear equation based on the modified Lambert-Beer law. To solve this equation, we need to know the partial optical pathl...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3147402
更新日期:2009-05-01 00:00:00
abstract::Diffuse optical spectroscopy (DOS) provides a powerful tool for fast and noninvasive disease diagnosis. The ability to leverage DOS to accurately quantify tissue optical parameters hinges on the model used to estimate light-tissue interaction. We describe the accuracy of a lookup table (LUT)-based inverse model for me...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.5.057001
更新日期:2012-05-01 00:00:00
abstract:SIGNIFICANCE:Despite the importance of the cell membrane in regulation of drug activity, the influence of drug treatments on its physical properties is still poorly understood. The combination of fluorescence lifetime imaging microscopy (FLIM) with specific viscosity-sensitive fluorescent molecular rotors allows the qu...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.25.12.126004
更新日期:2020-12-01 00:00:00
abstract::The reflection and transmission of coherent light from a biological system can yield information about its condition. In the case of blood exposed to the air, there is a change in the properties of the speckle patterns observed in the coagulation process. This can be studied by means of the rate of temporal variation,...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.24.3.031018
更新日期:2018-12-01 00:00:00