Abstract:
:A compact handpiece combining high resolution fluorescence (HRF) imaging with optical coherence tomography (OCT) was developed to provide real-time assessment of oral lesions. This multimodal imaging device simultaneously captures coregistered en face images with subcellular detail alongside cross-sectional images of tissue microstructure. The HRF imaging acquires a 712 × 594 μm² field-of-view at the sample with a spatial resolution of 3.5 μm. The OCT images were acquired to a depth of 1.5 mm with axial and lateral resolutions of 9.3 and 8.0 μm, respectively. HRF and OCT images are simultaneously displayed at 25 fps. The handheld device was used to image a healthy volunteer, demonstrating the potential for in vivo assessment of the epithelial surface for dysplastic and neoplastic changes at the cellular level, while simultaneously evaluating submucosal involvement. We anticipate potential applications in real-time assessment of oral lesions for improved surveillance and surgical guidance.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Higgins LM,Pierce MCdoi
10.1117/1.JBO.19.8.086004subject
Has Abstractpub_date
2014-08-01 00:00:00pages
086004issue
8eissn
1083-3668issn
1560-2281pii
1896336journal_volume
19pub_type
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journal_title:Journal of biomedical optics
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