Abstract:
:Ophthalmic surgery may benefit from use of more precise fiber delivery systems during laser surgery. Some current ophthalmic surgical techniques rely on tedious mechanical dissection of tissue layers. In this study, chains of sapphire microspheres integrated into a hollow waveguide distal tip are used for erbium:YAG laser ablation studies in contact mode with ophthalmic tissues, ex vivo. The laser's short optical penetration depth combined with the small spot diameters achieved with this fiber probe may provide more precise tissue removal. One-, three-, and five-microsphere chain structures were characterized, resulting in FWHM diameters of 67, 32, and 30 μm in air, respectively, with beam profiles comparable to simulations. Single Er:YAG pulses of 0.1 mJ and 75-μs duration produced ablation craters with average diameters of 44, 30, and 17 μm and depths of 26, 10, and 8 μm, for one-, three-, and five-sphere structures, respectively. Microsphere chains produced spatial filtering of the multimode Er:YAG laser beam and fiber, providing spot diameters not otherwise available with conventional fiber systems. Because of the extremely shallow treatment depth, compact focused beam, and contact mode operation, this probe may have potential for use in dissecting epiretinal membranes and other ophthalmic tissues without damaging adjacent retinal tissue.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Hutchens TC,Darafsheh A,Fardad A,Antoszyk AN,Ying HS,Astratov VN,Fried NMdoi
10.1117/1.JBO.17.6.068004subject
Has Abstractpub_date
2012-06-01 00:00:00pages
068004issue
6eissn
1083-3668issn
1560-2281journal_volume
17pub_type
杂志文章abstract::A simultaneous multispectral fluorescence imaging system incorporating multiplexed volume holographic grating (VHG) is developed to acquire multispectral images of an object in one shot. With the multiplexed VHG, the imaging system can provide the distribution and spectral characteristics of multiple fluorophores in t...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.6.060502
更新日期:2016-06-01 00:00:00
abstract::Microvascular network of the retina plays an important role in diagnosis and monitoring of various retinal diseases. We propose a three-dimensional (3-D) segmentation method with intensity-based Doppler variance (IBDV) based on swept-source optical coherence tomography. The automatic 3-D segmentation method is used to...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.7.076003
更新日期:2015-07-01 00:00:00
abstract::Many optical diagnostic approaches rely on changes in scattering and absorption properties to generate optical contrast between normal and diseased tissue. Recently, there has been increasing interest in using exogenous agents to enhance this intrinsic contrast with particular emphasis on the development for targeting...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2141825
更新日期:2005-11-01 00:00:00
abstract::Renal ischemia that occurs intraoperatively during procedures requiring clamping of the renal artery (such as renal procurement for transplantation and partial nephrectomy for renal cancer) is known to have a significant impact on the viability of that kidney. To better understand the dynamics of intraoperative renal ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.3.035001
更新日期:2013-03-01 00:00:00
abstract::Surgical excision of the whole prostate through a radical prostatectomy procedure is part of the standard of care for prostate cancer. Positive surgical margins (cancer cells having spread into surrounding nonresected tissue) occur in as many as 1 in 5 cases and strongly correlate with disease recurrence and the requi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.24.2.025001
更新日期:2019-02-01 00:00:00
abstract::We present experiments and analyses of confocal reflectance and two-photon microscopy studies of zebra finch skull samples. The thin and hollow structure of these birds' skulls is quite translucent, which can allow in vivo transcranial two-photon imaging for brain activation monitoring. However, the skull structure is...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3155522
更新日期:2009-05-01 00:00:00
abstract::Pulse oximeters are widely used for noninvasive monitoring of oxygen saturation in arterial blood hemoglobin. We present a transmittance pulse oximetry system based on near-infrared (NIR) laser diodes (750 and 850 nm) for monitoring oxygen saturation of arterial blood hemoglobin. The pulse oximetry system is made up o...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1578495
更新日期:2003-07-01 00:00:00
abstract::Diffuse optical imaging is an effective technique for noninvasive functional brain imaging. However, the measurements respond to systemic hemodynamic fluctuations caused by the cardiac cycle, respiration, and blood pressure, which may obscure or overwhelm the desired stimulus-evoked response. Previous work on this pro...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1852552
更新日期:2005-01-01 00:00:00
abstract::A Monte Carlo algorithm is applied to simulate the measurements of a sample with glass slides sandwiched between the double integrating sphere (DIS) setup. The effects caused by various parameters, such as the sample port of integrating sphere, thicknesses, and optical properties of the sample, on light losses and opt...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2815691
更新日期:2007-11-01 00:00:00
abstract::Diffusion of therapeutic macromolecules through the extracellular matrix of tumor tissue is a crucial step in drug delivery. We use fluorescence correlation spectroscopy (FCS) to measure diffusion of IgG (150 kDa) and dextrans (155 kDa and 2 MDa) in solution, 5% gelatin hydrogel, and multicellular spheroids. Gel and s...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2982530
更新日期:2008-09-01 00:00:00
abstract::This study demonstrates the use of optical spectroscopy for monitoring tumor oxygenation and metabolism in response to hyperoxic gas breathing. Hemoglobin saturation and redox ratio were quantified for a set of 14 and 9 mice, respectively, measured at baseline and during carbogen breathing (95% O(2), 5% CO(2)). In par...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3103586
更新日期:2009-03-01 00:00:00
abstract::A new skin-imaging method, which was developed recently, gives an informative image data about skin moles by collecting scattered polarized light reflected from their tissue area at different wavelengths and polarizations. This method is called as optical rotational spectropolarimetric imaging (ORSI), which scans the ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.11.111403
更新日期:2013-11-01 00:00:00
abstract::Fluorescent proteins and dyes are routine tools for biological research to describe the behavior of genes, proteins, and cells, as well as more complex physiological dynamics such as vessel permeability and pharmacokinetics. The use of these probes in whole body in vivo imaging would allow extending the range and scop...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.2.026009
更新日期:2016-02-01 00:00:00
abstract::Kinetics of optical tweezers (OT)-induced spontaneous aggregation and disaggregation of red blood cells (RBCs) were studied at the level of cell doublets to assess RBC interaction mechanics. Measurements were performed under in vitro conditions in plasma and fibrinogen and fibrinogen + albumin solutions. The RBC spont...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.3.035001
更新日期:2016-03-01 00:00:00
abstract::The attenuation of near-infrared (NIR) light intensity as it propagates in a turbid medium like biological tissue is described by modified the Beer–Lambert law (MBLL). The MBLL is generally used to quantify the changes in tissue chromophore concentrations for NIR spectroscopic data analysis. Even though MBLL is effect...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.7.076012
更新日期:2016-07-01 00:00:00
abstract::We demonstrate that doubly-resonant coherent anti-Stokes Raman scattering can provide enhanced and highly specific contrast for molecules containing unique Raman-active small molecular groups. This combination provides contrast for molecules that can otherwise be difficult to discriminate by Raman spectroscopy. Here, ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3544585
更新日期:2011-02-01 00:00:00
abstract::Vascular leakage is an important feature of various disease conditions. In vivo optical imaging provides a great opportunity for the evaluation of this phenomenon. In the present study, we focus on the development and validation of a near-infrared (NIR) imaging formula to allow reliable, cost-efficient evaluation of v...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.1.016022
更新日期:2015-01-01 00:00:00
abstract::Ex vivo human skin has been used extensively for cosmeceutical and drug delivery studies, transplantable skin allografts, or skin flaps. However, it has a half-life of a few days due to ischemic necrosis. Traditional methods of assessing viability can be time-consuming and provide limited metabolic information. Using ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3466580
更新日期:2010-07-01 00:00:00
abstract::We present methods for visualizing the dynamic response of biological samples to laser-induced heating. Our approach utilizes optical frequency-domain imaging to detect, spatially localize, and monitor unique dynamic signatures that arise within zones of active tissue denaturation. Since this information is precisely ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2714027
更新日期:2007-03-01 00:00:00
abstract::Under ultraviolet and visible light excitation, melanin is essentially a nonfluorescent substance. This work reports our study on near-infrared (NIR) fluorescence properties of melanins, and explores potential applications of NIR fluorescence techniques for evaluating skin disorders involving melanin. The NIR fluoresc...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2204007
更新日期:2006-05-01 00:00:00
abstract::We present unsupervised clustering methods for automatic grouping of human red blood cells (RBCs) extracted from RBC quantitative phase images obtained by digital holographic microscopy into three RBC clusters with regular shapes, including biconcave, stomatocyte, and sphero-echinocyte. We select some good features re...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.7.076015
更新日期:2017-07-01 00:00:00
abstract::Frequency domain optical coherence tomography (FD-OCT), based on an all-reflective high-speed InGaAs spectrometer, operating in the 1050 nm wavelength region for retinal diagnostics, enables high-speed, volumetric imaging of retinal pathologies with greater penetration into choroidal tissue is compared to conventional...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2773728
更新日期:2007-07-01 00:00:00
abstract::Peripheral nerve injury in vivo promotes a regenerative growth in vitro characterized by an improved neurite regrowth. Knowledge of the conditioning injury effects on both morphology and mechanical properties of live sensory neurons could be instrumental to understand the cellular and molecular mechanisms leading to t...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.10.106014
更新日期:2013-10-01 00:00:00
abstract::Photodynamic therapy (PDT) is an alternative to radical surgical resection for T1a or nonresectable carcinomas of the gastroesophageal junction. Besides the concentration of the photosensitizer, the light distribution in tissue is responsible for tumor destruction. For this reason, knowledge about the behavior of ligh...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2564793
更新日期:2007-01-01 00:00:00
abstract::For a long time, the antioxidative status in cattle has been discussed as an indicator for stress conditions resulting from disease or exertion. Until now, invasive approaches have been necessary to obtain blood samples or biopsy materials and gain insights into the antioxidative status of cattle. Due to these efforts...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.10.101514
更新日期:2012-10-01 00:00:00
abstract::Scanning laser ophthalmoscopy (SLO) and optical coherence tomography (OCT) provide complementary views of the retina, with the former collecting fluorescence data with good lateral but relatively low-axial resolution, and the latter collecting label-free backscattering data with comparable lateral but much higher axia...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.12.126005
更新日期:2015-01-01 00:00:00
abstract::Investigating the elasticity of ocular tissue (cornea and intraocular lens) could help the understanding and management of pathologies related to biomechanical deficiency. In previous studies, we introduced a setup based on optical coherence tomography for shear wave elastography (SWE) with high resolution and high se...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.1.016001
更新日期:2015-01-01 00:00:00
abstract::Type I collagen is a major component of the extracellular matrix as well as many tissue engineered models. To understand changes in collagen related models over time, it is important to evaluate collagen dynamics with noninvasive techniques. Fluorescence spectroscopy provides a method to noninvasively measure endogeno...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2360516
更新日期:2006-09-01 00:00:00
abstract::In breast surgery, a lack of knowledge about what is below the tissue surface may lead to positive tumor margins and iatrogenic damage. Diffuse reflectance spectroscopy (DRS) is a spectroscopic technique that can distinguish between healthy and tumor tissue making it a suitable technology for intraoperative guidance. ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.23.11.115004
更新日期:2018-11-01 00:00:00
abstract::Reactive oxygen species (ROS) produced by photodynamic therapy (PDT) is recorded in vivo using a chemiluminescence (CL)-based gated optical system. A novel approach is developed to utilize the fluorescence (FL) of the CL probe as an internal fluorescence to calibrate the observed CL on pharmacokinetics of the probe in...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3368688
更新日期:2010-03-01 00:00:00