Abstract:
:In breast surgery, a lack of knowledge about what is below the tissue surface may lead to positive tumor margins and iatrogenic damage. Diffuse reflectance spectroscopy (DRS) is a spectroscopic technique that can distinguish between healthy and tumor tissue making it a suitable technology for intraoperative guidance. However, because tumor surgeries are often performed with an electrosurgical knife, the effect of a coagulated tissue layer on DRS measurements must be taken into account. It is evaluated whether real-time DRS measurements obtained with a photonic electrosurgical knife could provide useful information of tissue properties also when tissue is coagulated and cut. The size of the coagulated area is determined and the effect of its presence on DR spectra is studied using ex vivo porcine adipose and muscle tissue. A coagulated tissue layer with a depth of 0.1 to 0.4 mm is observed after coagulating muscle with an electrosurgical knife. The results show that the effect of coagulating adipose tissue is negligible. Using the fat/water ratio's calculated from the measured spectra of the photonic electrosurgical knife, it was possible to determine the distance from the instrument tip to a tissue transition during cutting. In conclusion, the photonic electrosurgical knife can determine tissue properties of coagulated and cut tissue and has, therefore, the potential to provide real-time feedback about the presence of breast tumor margins during cutting, helping surgeons to establish negative margins and improve patient outcome.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Adank MW,Fleischer JC,Dankelman J,Hendriks BHWdoi
10.1117/1.JBO.23.11.115004subject
Has Abstractpub_date
2018-11-01 00:00:00pages
1-10issue
11eissn
1083-3668issn
1560-2281pii
JBO-180281RRjournal_volume
23pub_type
杂志文章abstract::We propose an offset-sparsity decomposition method for the enhancement of a color microscopic image of a stained specimen. The method decomposes vectorized spectral images into offset terms and sparse terms. A sparse term represents an enhanced image, and an offset term represents a "shadow." The related optimization ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.7.076012
更新日期:2015-07-01 00:00:00
abstract::We describe a systematic approach to image, track, and quantify the movements of HIV viruses embedded in human cervical mucus. The underlying motivation for this study is that, in HIV-infected adults, women account for more than half of all new cases and most of these women acquire the infection through heterosexual c...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.9.096001
更新日期:2016-09-01 00:00:00
abstract::Ambulatory diffuse optical tomography (aDOT) is based on near-infrared spectroscopy (NIRS) and enables three-dimensional imaging of regional hemodynamics and oxygen consumption during a person’s normal activities. Although NIRS has been previously used for muscle assessment, it has been notably limited in terms of the...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.9.091314
更新日期:2016-09-01 00:00:00
abstract::We report noninvasive, in vivo optical imaging deep within a mouse brain by swept-source optical coherence tomography (SS-OCT), enabled by a 1.3-μm vertical cavity surface emitting laser (VCSEL). VCSEL SS-OCT offers a constant signal sensitivity of 105 dB throughout an entire depth of 4.25 mm in air, ensuring an exten...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.10.106004
更新日期:2015-10-01 00:00:00
abstract::A hyperspectral image projector (HIP) based on liquid crystal on silicon spatial light modulators is explained and demonstrated to generate data cubes. The HIP-constructed data cubes are three-dimensional images of the spatial distribution of spectrally resolved abundances of intracellular light-absorbing oxyhemoglobi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,收录出版
doi:10.1117/1.JBO.20.11.111215
更新日期:2015-01-01 00:00:00
abstract::Compact optical imagers that can detect both spectral and polarization signatures are required in many biomedical applications. An acousto-optic-tunable-filter (AOTF)-based imager is ideally suited to provide both agile spectral and polarization signatures. Such an imager can be readily used for real-time in vivo medi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2102507
更新日期:2005-09-01 00:00:00
abstract::Photodynamic therapy (PDT) is an alternative to radical surgical resection for T1a or nonresectable carcinomas of the gastroesophageal junction. Besides the concentration of the photosensitizer, the light distribution in tissue is responsible for tumor destruction. For this reason, knowledge about the behavior of ligh...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2564793
更新日期:2007-01-01 00:00:00
abstract::We investigate optoelectronic properties of integrated structures comprising semiconductor light-emitting materials for optical probes of microscopic biological systems. Compound semiconductors are nearly ideal light emitters for probing cells and other microorganisms because of their spectral match to the transparenc...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1506931
更新日期:2002-10-01 00:00:00
abstract::The use of fluorescence video imaging to guide surgery is rapidly expanding, and improvements in camera readout dynamic range have not matched display capabilities. Logarithmic intensity compression is a fast, single-step mapping technique that can map the useable dynamic range of high-bit fluorescence images onto the...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.8.080504
更新日期:2015-08-01 00:00:00
abstract::The main curative therapy for patients with nonsmall cell lung cancer is surgery. Despite this, the survival rate is only 50%, therefore it is important to more efficiently diagnose and predict prognosis for lung cancer patients. Raman spectroscopy is useful in the diagnosis of malignant and premalignant lesions. The ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3323088
更新日期:2010-03-01 00:00:00
abstract::Modern gynecologic practice requires noninvasive diagnostics techniques capable of detecting morphological and functional alterations in tissues of female reproductive organs. Optical coherence tomography (OCT) is a promising tool for providing imaging of biotissues with high resolution at depths up to 2 mm. Design of...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.JBO.22.12.121709
更新日期:2017-12-01 00:00:00
abstract::The recycling of G-protein-coupled receptors (GPCR) to the cell surface after internalization plays an important role in the regulation of overall GPCR activity. The angiotensin II type I receptor (AT1R) belongs to class B GPCRs that recycle slowly back to the cell surface. Previous studies have proposed that Rab11 co...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2943286
更新日期:2008-05-01 00:00:00
abstract::The study of red blood cell (RBC) aggregation is of great interest because of its implications for human health. Altered RBC aggregation can lead to microcirculatory problems as in vascular pathologies, such as hypertension and diabetes, due to a decrease in the erythrocyte surface electric charge and an increase in t...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.1.017003
更新日期:2017-01-01 00:00:00
abstract::The infrared and Raman spectroscopy of bone and teeth tissues are reviewed. Characteristic spectra are obtained for both the mineral and protein components of these tissues. Vibrational spectroscopy is used to study the mineralization process, to define the chemical structure changes accompanying bone diseases, and to...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.429994
更新日期:2000-07-01 00:00:00
abstract:SIGNIFICANCE:Melanoma is a deadly cancer that physicians struggle to diagnose early because they lack the knowledge to differentiate benign from malignant lesions. Deep machine learning approaches to image analysis offer promise but lack the transparency to be widely adopted as stand-alone diagnostics. AIM:We aimed to...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.25.11.112906
更新日期:2020-11-01 00:00:00
abstract::A computer simulation called RHME (Retinal Heating in Moving Eye) is developed to simulate the heating pattern that occurs in the retina during a long-duration exposure to a continuous wave laser beam. The simulation takes into account eye movements that occur during a deliberate fixation. Due to the rapid (millisecon...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1783355
更新日期:2004-09-01 00:00:00
abstract::A new, simple, high-speed, and hardware-only integration-based fluorescence-lifetime-sensing algorithm using a center-of-mass method (CMM) is proposed to implement lifetime calculations, and its signal-to-noise-ratio based on statistics theory is also deduced. Compared to the commonly used iterative least-squares meth...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3309737
更新日期:2010-01-01 00:00:00
abstract::The absorption coefficient mu(a), scattering coefficient mu(s), and anisotropy factor g of diluted and undiluted human blood (hematocrit 0.84 and 42.1%) are determined under flow conditions in the wavelength range 250 to 1100 nm, covering the absorption bands of hemoglobin. These values are obtained by high precision ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2203659
更新日期:2006-05-01 00:00:00
abstract::The use of reduced nicotinamide adenine dinucleotide (NADH) fluorescence to gain metabolic information on kidneys in response to an alteration in oxygen availability has previously been experimentally demonstrated, but signal quantification has not, to date, been addressed. In this work the relative contribution to ra...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3094948
更新日期:2009-03-01 00:00:00
abstract::Alzheimer's disease (AD) is a neurodegenerative disorder that leads to a decline in cognitive and intellectual abilities and an irreversible mental deterioration. Based on multidisciplinary AD research, the most universally accepted hypotheses on AD pathogenesis are the intracerebral aggregate formation of beta-amyloi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.5.051205
更新日期:2014-05-01 00:00:00
abstract::A method for the prediction of the average photon pathlength in turbid media has been developed. The method is based on spatially resolved diffuse reflectance with discrete source detector distances up to 2 mm. Light reflectance was simulated using a Monte Carlo technique with a one-layer model utilizing a wide range ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1482378
更新日期:2002-07-01 00:00:00
abstract::Recaldent is a product of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP). The remineralizing potential of CPP-ACP per se, or when combined with 0.22% Fl gel on artificially demineralized enamel using laser florescence, is investigated. Mesial surfaces of 15 sound human molars are tested using a He-Cd lase...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3210780
更新日期:2009-07-01 00:00:00
abstract::As the most abundant cell type in the central nervous system, astrocyte has been one of main research topics in neuroscience. Although various tools have been developed, at present, there is no tool that allows noninvasive activation of astrocyte in vivo without genetic or pharmacological perturbation. Here we report ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3600774
更新日期:2011-07-01 00:00:00
abstract::Cell morphology is often used as a valuable indicator of the physical condition and general status of living cells. We demonstrate a noninvasive method for morphological characterization of adherent cells. We measure infrared reflectivity spectrum at oblique angle from living cells cultured on thin Au film, and utiliz...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.8.081409
更新日期:2012-08-01 00:00:00
abstract::A multifunctional line scanning ophthalmoscope (mLSO) was designed, constructed, and tested on human subjects. The mLSO could sequentially acquire wide-field, confocal, near-infrared reflectance, fluorescein angiography (FA), and indocyanine green angiography (ICGA) retinal images. The system also included a retinal t...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.2.026008
更新日期:2012-02-01 00:00:00
abstract::Near-infrared diffuse optical tomography (DOT), one of the most sophisticated optical imaging techniques for observations through biological tissue, allows 3-D quantitative imaging of optical properties, which include functional and anatomical information. With DOT, it is expected to be possible to overcome the limita...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.JBO.21.9.091312
更新日期:2016-09-01 00:00:00
abstract::Photobiological research in the last 30 yr has shown the existence of ultraweak photon emission in biological tissue, which can be detected with sophisticated photomultiplier systems. Although the emission of this ultraweak radiation, often termed biophotons, is extremely low in mammalian cells, it can be efficiently ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1899185
更新日期:2005-03-01 00:00:00
abstract::The composition of cartilage is predictive of its in vivo performance. Therefore, the ability to assess its primary macromolecular components, proteoglycan (PG) and collagen, is of great importance. In the current study, we hypothesized that PG content and distribution in tissue engineered cartilage could be determine...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1922329
更新日期:2005-05-01 00:00:00
abstract::The capability of optical coherence tomography (OCT) to perform "optical biopsy" of tissues within a depth range of 1 to 2 mm with micron-scale resolution in real time makes it a promising biomedical imaging modality for dermatologic applications. Three high-speed, spectrometer-based frequency-domain OCT systems opera...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3400665
更新日期:2010-03-01 00:00:00
abstract::We propose a handheld projective imaging device for orthotopic projection of near-infrared fluorescence images onto target biological tissue at visible wavelengths without any additional visual aid. The device integrates a laser diode light source module, a camera module, a projector, an ultrasonic distance sensor, a ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.24.8.080503
更新日期:2019-08-01 00:00:00