Abstract:
:We describe a systematic approach to image, track, and quantify the movements of HIV viruses embedded in human cervical mucus. The underlying motivation for this study is that, in HIV-infected adults, women account for more than half of all new cases and most of these women acquire the infection through heterosexual contact. The endocervix is believed to be a susceptible site for HIV entry. Cervical mucus, which coats the endocervix, should play a protective role against the viruses. Thus, we developed a methodology to apply time-resolved confocal microscopy to examine the motion of HIV viruses that were added to samples of untreated cervical mucus. From the images, we identified the viruses, tracked them over time, and calculated changes of the statistical mean-squared displacement (MSD) of each virus. Approximately half of tracked viruses appear constrained while the others show mobility with MSDs that are proportional to ??+?2?2, over time range ?, depicting a combination of anomalous diffusion (0<0.40) and flow-like behavior. The MSD data also reveal plateaus attributable to possible stalling of the viruses. Although a more extensive study is warranted, these results support the assumption of mucus being a barrier against the motion of these viruses.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Boukari F,Makrogiannis S,Nossal R,Boukari Hdoi
10.1117/1.JBO.21.9.096001subject
Has Abstractpub_date
2016-09-01 00:00:00pages
96001issue
9eissn
1083-3668issn
1560-2281pii
2551780journal_volume
21pub_type
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