Statistical strategies to reveal potential vibrational markers for in vivo analysis by confocal Raman spectroscopy.

abstract：:Doxorubicin is a commonly used chemotherapeutic employed to treat multiple human cancers, including numerous sarcomas and carcinomas. Furthermore, doxorubicin possesses strong fluorescent properties that make it an ideal reagent for modeling drug delivery by examining its distribution in cells and tissues. However, wh...

journal_title：Journal of biomedical optics

authors： Carlson M,Watson AL,Anderson L,Largaespada DA,Provenzano PP

更新日期：2017-11-01 00:00:00

abstract：:Given the several difficulties associated with histology, including difficulty in continuous monitoring, this study aimed to investigate the feasibility of optical imaging modalities—cross-polarization color (CPC) imaging, erythema index (EI) imaging, and laser speckle contrast (LSC) imaging—for continuous evaluation ...

journal_title：Journal of biomedical optics

authors： Son T,Yoon HJ,Lee S,Jang WS,Jung B,Kim WU

更新日期：2014-01-01 00:00:00

abstract：:Photobiological research in the last 30 yr has shown the existence of ultraweak photon emission in biological tissue, which can be detected with sophisticated photomultiplier systems. Although the emission of this ultraweak radiation, often termed biophotons, is extremely low in mammalian cells, it can be efficiently ...

journal_title：Journal of biomedical optics

authors： Niggli HJ,Tudisco S,Privitera G,Applegate LA,Scordino A,Musumeci F

更新日期：2005-03-01 00:00:00

abstract：:A method for the prediction of the average photon pathlength in turbid media has been developed. The method is based on spatially resolved diffuse reflectance with discrete source detector distances up to 2 mm. Light reflectance was simulated using a Monte Carlo technique with a one-layer model utilizing a wide range ...

journal_title：Journal of biomedical optics

authors： Nilsson H,Larsson M,Nilsson GE,Strömberg T

更新日期：2002-07-01 00:00:00

abstract：:We demonstrate that doubly-resonant coherent anti-Stokes Raman scattering can provide enhanced and highly specific contrast for molecules containing unique Raman-active small molecular groups. This combination provides contrast for molecules that can otherwise be difficult to discriminate by Raman spectroscopy. Here, ...

journal_title：Journal of biomedical optics

authors： Weeks T,Schie I,den Hartigh LJ,Rutledge JC,Huser T

更新日期：2011-02-01 00:00:00

abstract：:Dental erosion starts with a chemical attack on dental tissue causing tooth demineralization, altering the tooth structure and making it more sensitive to mechanical erosion. Medical diagnosis of dental erosion is commonly achieved through a visual inspection by the dentist during dental checkups and is therefore high...

journal_title：Journal of biomedical optics

authors： Abou Nader C,Pellen F,Loutfi H,Mansour R,Le Jeune B,Le Brun G,Abboud M

更新日期：2016-07-01 00:00:00

abstract：:Parametric and nonparametric data processing schemes for analyzing translating laser speckle data used to investigate the mechanical behavior of biological tissues are examined. Cross-correlation, minimum mean square estimator, maximum likelihood, and maximum entropy approaches are discussed and compared on speckle da...

journal_title：Journal of biomedical optics

authors： Duncan DD,Kirkpatrick SJ

更新日期：2001-10-01 00:00:00

abstract：:Noninvasive cardiovascular imaging could lead to the early detection and timely treatment of complex atherosclerotic lesions responsible for major cardiovascular events. Recent investigations have suggested that optical coherence tomography (OCT) is an ideal diagnostic tool due to the high resolution this technology a...

journal_title：Journal of biomedical optics

authors： Ghosn MG,Carbajal EF,Befrui NA,Tellez A,Granada JF,Larin KV

更新日期：2008-01-01 00:00:00

abstract：:Dispersion encoded full-range (DEFR) frequency-domain optical coherence tomography (FD-OCT) and its enhanced version, fast DEFR, utilize dispersion mismatch between sample and reference arm to eliminate the ambiguity in OCT signals caused by non-complex valued spectral measurement, thereby numerically doubling the usa...

journal_title：Journal of biomedical optics

authors： Wang L,Hofer B,Guggenheim JA,Povazay B

更新日期：2012-07-01 00:00:00

abstract：:We introduce the concept of deliberate placement of absorbers to alter the average path of photons through tissue for a biomedical optical device. By changing the reflectivity of a needle that separates a source and detector, the average photon path through a turbid medium can be changed. Totally reflective needles ha...

journal_title：Journal of biomedical optics

authors： Simonson P,D'Amico E,Gratton E

更新日期：2006-01-01 00:00:00

abstract：SIGNIFICANCE:The blood-brain barrier (BBB) is a major obstacle to detecting and treating brain tumors. Overcoming this challenge will facilitate the early and accurate detection of brain lesions and guide surgical resection of tumors. AIM:We generated an orthotopic brain tumor model that simulates the pathophysiology ...

journal_title：Journal of biomedical optics

authors： Habimana-Griffin L,Ye D,Carpenter J,Prior J,Sudlow G,Marsala L,Mixdorf M,Rubin J,Chen H,Achilefu S

更新日期：2020-02-01 00:00:00

abstract：:Fluorescence spectroscopy has been widely investigated as a technique for identifying pathological tissue; however, unrelated subject-to-subject variations in spectra complicate data analysis and interpretation. We describe and evaluate a new biosensing technique, differential laser-induced perturbation spectroscopy (...

journal_title：Journal of biomedical optics

authors： Kozikowski RT,Smith SE,Lee JA,Castleman WL,Sorg BS,Hahn DW

更新日期：2012-06-01 00:00:00

abstract：:We present a quantitative analysis of dynamic diffuse optical measurements to obtain oxygen saturation of hemoglobin in volume oscillating compartments. We used a phasor representation of oscillatory hemodynamics at the heart rate and respiration frequency to separate the oscillations of tissue concentrations of oxyhe...

journal_title：Journal of biomedical optics

authors： Kainerstorfer JM,Sassaroli A,Fantini S

更新日期：2016-10-01 00:00:00

abstract：:We report that the pathologic components present within the atheromatous plaques of ApoE knock-out mice can reflect significant amounts of mid-infrared (mid-IR) light. Furthermore, the reflected light spectra contained the unique signatures of a variety of biologic features including those found in unstable or "vulner...

journal_title：Journal of biomedical optics

authors： Holman HY,Bjornstad KA,Martin MC,McKinney WR,Blakely EA,Blankenberg FG

更新日期：2008-05-01 00:00:00

abstract：:The capability of optical coherence tomography (OCT) to perform "optical biopsy" of tissues within a depth range of 1 to 2 mm with micron-scale resolution in real time makes it a promising biomedical imaging modality for dermatologic applications. Three high-speed, spectrometer-based frequency-domain OCT systems opera...

journal_title：Journal of biomedical optics

authors： Alex A,Povazay B,Hofer B,Popov S,Glittenberg C,Binder S,Drexler W

更新日期：2010-03-01 00:00:00

abstract：:Application of a fiber optic biosensor (FOB) to the real-time investigation of the interaction kinetics between FITC-conjugated monoclonal sheep anti-human C-reactive protein (CRP) antibody and CRP isoforms on the surface of optical fiber is described. Recently, both the native pentameric CRP (pCRP), an acute phase pr...

journal_title：Journal of biomedical optics

authors： Chou C,Hsu HY,Wu HT,Tseng KY,Chiou A,Yu CJ,Lee ZY,Chan TS

更新日期：2007-03-01 00:00:00

abstract：:Optical-resolution photoacoustic microscopy (ORPAM) in theory provides lateral resolution equivalent to the optical diffraction limit. Scattering media, such as biological turbid media, attenuates the optical signal and also alters the diffraction-limited spot size of the focused beam. The ORPAM signal is generated on...

journal_title：Journal of biomedical optics

authors： U-Thainual P,Kim DH

更新日期：2015-12-01 00:00:00

abstract：:Fiber-guided ablation of soft tissue with pulsed holmium and thulium lasers was investigated for intraluminal incisions. A bare fiber/tissue-contact application system with a nearly tangential irradiation geometry was first used in vitro on porcine ureter tissue. The efficiency and precision of the method was analyzed...

journal_title：Journal of biomedical optics

authors： Brinkmann R,Knipper A,Dro Ge G,Schro Er F,Gromoll B,Birngruber R

更新日期：1998-01-01 00:00:00

abstract：:As molecular imaging moves towards lower detection limits, the elimination of endogenous background signals becomes imperative. We present a facile background-suppression technique that specifically segregates the signal from surface-enhanced Raman scattering (SERS)-active nanoparticles (NPs) from the tissue autofluor...

journal_title：Journal of biomedical optics

authors： Mallia RJ,McVeigh PZ,Veilleux I,Wilson BC

更新日期：2012-07-01 00:00:00

abstract：:Temperature distribution is a crucial factor in determining the outcome of laser phototherapy in cancer treatment. Magnetic resonance imaging (MRI) is an ideal method for 3-D noninvasive temperature measurement. A 7.1-T MRI was used to determine laser-induced high thermal gradient temperature distribution of target ti...

journal_title：Journal of biomedical optics

authors： Chen Y,Gnyawali SC,Wu F,Liu H,Tesiram YA,Abbott A,Towner RA,Chen WR

更新日期：2008-07-01 00:00:00

abstract：:Radiotherapy is one of the main methods to treat cancer. However, due to the propagation pattern of high-energy photons in tissue and their inability to discriminate between healthy and malignant tissues, healthy tissues may also be damaged, causing undesired side effects. A possible method for internal electron thera...

journal_title：Journal of biomedical optics

authors： Tepper M,Barkai U,Gannot I

更新日期：2015-05-01 00:00:00

abstract：:Intraoperative applications of near-infrared (NIR) fluorescent contrast agents can be aided by instrumentation capable of merging the view of surgical field with that of NIR fluorescence. We demonstrate augmented microscopy, an intraoperative imaging technique in which bright-field (real) and electronically processed ...

journal_title：Journal of biomedical optics

authors： Watson JR,Gainer CF,Martirosyan N,Skoch J,Lemole GM Jr,Anton R,Romanowski M

更新日期：2015-10-01 00:00:00

abstract：:We have developed a novel nitroimidazole indocyanine dye conjugate for tumor-targeted hypoxia fluorescence tomography. The hypoxia probe has been evaluated in vitro using tumor cell lines and in vivo with tumor targeting in mice. The in vitro cell studies were performed to assess fluorescence labeling differences betw...

journal_title：Journal of biomedical optics

authors： Biswal NC,Pavlik C,Smith MB,Aguirre A,Xu Y,Zanganeh S,Kuhn LT,Claffey KP,Zhu Q

更新日期：2011-06-01 00:00:00

abstract：:Guest editors introduce contributors to the Special Section Celebrating the Exponential Growth of Optoacoustic/Photoacoustic Imaging. ...

journal_title：Journal of biomedical optics

authors： Beard P,Anastasio M,Oraevsky AA,Wang LV

更新日期：2019-12-01 00:00:00

abstract：:The expensive fabrication of current opto-microfluidic sensors is a barrier to the successful adoption of these devices in point-of-care testing. This work reports a simple inexpensive opto-microfluidic device incorporating a poly(dimethylsiloxane)-glass hybrid microfluidic chip modified with gold nanoparticles and a ...

journal_title：Journal of biomedical optics

authors： Pires NM,Dong T

更新日期：2014-03-01 00:00:00

abstract：:Micrometer scale resolution full-field optical coherence tomography (FF-OCT) is developed for imaging human graft corneas. Three-dimensional (3-D) images with ultrahigh resolution (respectively, 1 and 1.5 μm in the axial and transverse directions), comparable to traditional histological sections, are obtained allowing...

journal_title：Journal of biomedical optics

authors： Latour G,Georges G,Lamoine LS,Deumié C,Conrath J,Hoffart L

更新日期：2010-09-01 00:00:00

abstract：:Optically sectioned detection of fluorescence immunoassays using a confocal microscope enables the creation of both homo- and heterogeneous planar format assays. We report a set assays requiring optically sectioned detection using a model system and analysis procedures for separating signals of a surface layer from an...

journal_title：Journal of biomedical optics

authors： Ghafari H,Zhou Y,Ali S,Hanley QS

更新日期：2009-11-01 00:00:00

abstract：:We report a photonic approach for selective inactivation of viruses with a near-infrared subpicosecond laser. We demonstrate that this method can selectively inactivate viral particles ranging from nonpathogenic viruses such as the M13 bacteriophage and the tobacco mosaic virus to pathogenic viruses such as the human ...

journal_title：Journal of biomedical optics

authors： Tsen KT,Tsen SW,Fu Q,Lindsay SM,Kibler K,Jacobs B,Wu TC,Karanam B,Jagu S,Roden RB,Hung CF,Sankey OF,Ramakrishna B,Kiang JG

更新日期：2009-11-01 00:00:00

abstract：:Spectral resolved tissue imaging has a broad range of biomedical applications such as the minimally invasive diagnosis of diseases and the study of wound healing and tissue engineering processes. Two-photon microscopy imaging of endogenous fluorescence has been shown to be a powerful method for the quantification of t...

journal_title：Journal of biomedical optics

authors： Laiho LH,Pelet S,Hancewicz TM,Kaplan PD,So PT

更新日期：2005-03-01 00:00:00

abstract：:This study aimed to evaluate the concept of using high-resolution optical coherence tomography (OCT) imaging to rapidly assess surgical specimens and determine if cancer positive margins were left behind in the surgical bed. A mouse model of breast cancer was used in this study. Surgical specimens from 30 animals were...

journal_title：Journal of biomedical optics

authors： Savastru D,Chang EW,Miclos S,Pitman MB,Patel A,Iftimia N

更新日期：2014-05-01 00:00:00