Light scattering of human red blood cells during metabolic remodeling of the membrane.

abstract：:As the most abundant cell type in the central nervous system, astrocyte has been one of main research topics in neuroscience. Although various tools have been developed, at present, there is no tool that allows noninvasive activation of astrocyte in vivo without genetic or pharmacological perturbation. Here we report ...

journal_title：Journal of biomedical optics

authors： Choi M,Yoon J,Ku T,Choi K,Choi C

更新日期：2011-07-01 00:00:00

abstract：:In order to diagnose cancer, a sample must be removed, prepared, and examined under a microscope, which is expensive, invasive, and time consuming. Fiber optic fluorescence endomicroscopy, where an image guide is used to obtain high-resolution images of tissue in vivo, has shown promise as an alternative to convention...

journal_title：Journal of biomedical optics

authors： Kyrish M,Dobbs J,Jain S,Wang X,Yu D,Richards-Kortum R,Tkaczyk TS

更新日期：2013-09-01 00:00:00

abstract：:Optical quantification of large lesions imaged with diffuse optical tomography in reflection geometry is depth dependence due to the exponential decay of photon density waves. We introduce a depth-correction method that incorporates the target depth information provided by coregistered ultrasound. It is based on balan...

journal_title：Journal of biomedical optics

authors： Tavakoli B,Zhu Q

更新日期：2011-05-01 00:00:00

abstract：:In the present applications of optical coherence tomography (OCT), parameters besides pure morphology are evaluated in skin tissue under in vivo conditions. Spatially mapped refractive indices and scattering coefficients may support tissue characterization for research and diagnostic purposes in cosmetics/pharmacy and...

journal_title：Journal of biomedical optics

authors： Knüttel A,Boehlau-Godau M

更新日期：2000-01-01 00:00:00

abstract：:A main field in biomedical optics research is diffuse optical tomography, where intensity variations of the transmitted light traversing through tissue are detected. Mathematical models and reconstruction algorithms based on finite element methods and Monte Carlo simulations describe the light transport inside the tis...

journal_title：Journal of biomedical optics

authors： Baum K,Hartmann R,Bischoff T,Oelerich JO,Finkensieper S,Heverhagen JT

更新日期：2012-12-01 00:00:00

abstract：:Optical coherence tomography (OCT)-based elasticity imaging can map soft tissue elasticity based on speckle-tracking of elastic wave propagation using highly sensitive phase measurements of OCT signals. Using a fixed elastic wave source and moving detection, current imaging sequences have difficulty in reconstructing ...

journal_title：Journal of biomedical optics

authors： Hsieh BY,Song S,Nguyen TM,Yoon SJ,Shen TT,Wang RK,O'Donnell M

更新日期：2016-11-01 00:00:00

abstract：:The transmembrane protein prestin is crucial to outer hair cell (OHC) electromotility and contributes to the sensitivity and frequency selectivity of mammalian hearing. The molecular mechanisms of electromotility remain unclear, but prestin is purported to function as both a voltage sensor and a molecular motor. Under...

journal_title：Journal of biomedical optics

authors： Organ LE,Raphael RM

更新日期：2007-03-01 00:00:00

abstract：:To improve surgical guidance toward prostate draining lymph nodes, we investigate the potential of intraoperative fluorescence imaging and combined pre- and intraoperative multimodality imaging approaches. Transgenic adenocarcinoma mouse prostate mice with spontaneous prostate tumors are injected intratumorally with: ...

journal_title：Journal of biomedical optics

authors： van Leeuwen AC,Buckle T,Bendle G,Vermeeren L,Valdés Olmos R,van de Poel HG,van Leeuwen FW

更新日期：2011-01-01 00:00:00

abstract：:Polarized Raman spectroscopy allows measurement of molecular orientation and composition and is widely used in the study of polymer systems. Here, we extend the technique to the extraction of quantitative orientation information from bone tissue, which is optically thick and highly turbid. We discuss multiple scatteri...

journal_title：Journal of biomedical optics

authors： Raghavan M,Sahar ND,Wilson RH,Mycek MA,Pleshko N,Kohn DH,Morris MD

更新日期：2010-05-01 00:00:00

abstract：:We compared light-induced fluorescence (LIF) to nominal injected drug dose for predicting the depth of necrosis response to photodynamic therapy (PDT) in a murine tumor model. Mice were implanted with radiation-induced fibrosarcoma (RIF) and were injected with 0, 5, or 10 mg/kg Photofrin. 630-nm light (30 J/cm(2), 75 ...

journal_title：Journal of biomedical optics

authors： Cheung R,Solonenko M,Busch TM,Del Piero F,Putt ME,Hahn SM,Yodh AG

更新日期：2003-04-01 00:00:00

abstract：:Treatment of deep venous thrombosis (DVT)--a primary cause of potentially fatal pulmonary embolism (PE)--depends on the age of the thrombus. The existing clinical imaging methods are capable of visualizing a thrombus but cannot determine the age of the blood clot. Therefore, there is a need for an imaging technique to...

journal_title：Journal of biomedical optics

authors： Karpiouk AB,Aglyamov SR,Mallidi S,Shah J,Scott WG,Rubin JM,Emelianov SY

更新日期：2008-09-01 00:00:00

abstract：:The fibrillar collagen network in tumor and normal tissues is different due to remodeling of the extracellular matrix during the malignant process. Collagen type I fibers have the crystalline and noncentrosymmetric properties required for generating the second-harmonic signal. The content and structure of collagen wer...

journal_title：Journal of biomedical optics

authors： Hompland T,Erikson A,Lindgren M,Lindmo T,de Lange Davies C

更新日期：2008-09-01 00:00:00

abstract：:A microelectromechanical system (MEMS) mirror based endoscopic swept-source optical coherence tomography (SS-OCT) system that can perform three-dimensional (3-D) imaging at high speed is reported. The key component enabling 3-D endoscopic imaging is a two-axis MEMS scanning mirror which has a 0.8×0.8  mm2 mirror plate...

journal_title：Journal of biomedical optics

authors： Wang D,Fu L,Wang X,Gong Z,Samuelson S,Duan C,Jia H,Ma JS,Xie H

更新日期：2013-08-01 00:00:00

abstract：:Most ovarian cancers are diagnosed at advanced stages due to the lack of efficacious screening techniques. Photoacoustic tomography (PAT) has a potential to image tumor angiogenesis and detect early neovascular changes of the ovary. We have developed a coregistered PAT and ultrasound (US) prototype system for real-tim...

journal_title：Journal of biomedical optics

authors： Salehi HS,Li H,Merkulov A,Kumavor PD,Vavadi H,Sanders M,Kueck A,Brewer MA,Zhu Q

更新日期：2016-04-30 00:00:00

abstract：:Vascular leakage is an important feature of various disease conditions. In vivo optical imaging provides a great opportunity for the evaluation of this phenomenon. In the present study, we focus on the development and validation of a near-infrared (NIR) imaging formula to allow reliable, cost-efficient evaluation of v...

journal_title：Journal of biomedical optics

authors： Botz B,Bölcskei K,Kemény Á,Sándor Z,Tékus V,Sétáló G Jr,Csepregi J,Mócsai A,Pintér E,Kollár L,Helyes Z

更新日期：2015-01-01 00:00:00

abstract：:Intraoperative applications of near-infrared (NIR) fluorescent contrast agents can be aided by instrumentation capable of merging the view of surgical field with that of NIR fluorescence. We demonstrate augmented microscopy, an intraoperative imaging technique in which bright-field (real) and electronically processed ...

journal_title：Journal of biomedical optics

authors： Watson JR,Gainer CF,Martirosyan N,Skoch J,Lemole GM Jr,Anton R,Romanowski M

更新日期：2015-10-01 00:00:00

abstract：:A synchrotron light source dedicated to medical applications has been designed at National Institute of Radiological Sciences. The storage ring, with circumference of 80 m, is designed for acceleration of 2.3 GeV and a stored current of 420 mA. It is equipped with two multipole wigglers to produce sufficient photon fl...

journal_title：Journal of biomedical optics

authors： Torikoshi M,Tsunoo T,Endo M,Noda K,Kumada M,Yamada S,Soga F,Hyodo K

更新日期：2001-07-01 00:00:00

abstract：:A quantification method to measure endocytosis was designed to assess cellular uptake and specificity of a targeting nanoparticle platform. A simple N -hydroxysuccinimide ester conjugation technique to functionalize 100-nm hollow silica nanoshell particles with fluorescent reporter fluorescein isothiocyanate and folat...

journal_title：Journal of biomedical optics

authors： Sandoval S,Mendez N,Alfaro JG,Yang J,Aschemeyer S,Liberman A,Trogler WC,Kummel AC

更新日期：2015-08-01 00:00:00

abstract：:The lymphatic system is responsible for fluid homeostasis and immune cell trafficking and has been implicated in several diseases, including obesity, diabetes, and cancer metastasis. Despite its importance, the lack of suitable in vivo imaging techniques has hampered our understanding of the lymphatic system. This is,...

journal_title：Journal of biomedical optics

authors： Forbrich A,Heinmiller A,Zemp RJ

更新日期：2017-10-01 00:00:00

abstract：:Dental erosion starts with a chemical attack on dental tissue causing tooth demineralization, altering the tooth structure and making it more sensitive to mechanical erosion. Medical diagnosis of dental erosion is commonly achieved through a visual inspection by the dentist during dental checkups and is therefore high...

journal_title：Journal of biomedical optics

authors： Abou Nader C,Pellen F,Loutfi H,Mansour R,Le Jeune B,Le Brun G,Abboud M

更新日期：2016-07-01 00:00:00

abstract：:Nanophotothermolysis with long laser pulses for treatment of scattered cancer cells and their clusters is introduced with the main focus on real-time monitoring of temperature dynamics inside and around individual cancer cells labeled with carbon nanotubes. This technique utilizes advanced time- and spatially-resolved...

journal_title：Journal of biomedical optics

authors： Biris AS,Boldor D,Palmer J,Monroe WT,Mahmood M,Dervishi E,Xu Y,Li Z,Galanzha EI,Zharov VP

更新日期：2009-03-01 00:00:00

abstract：:We investigate the relationship between the laser beam at the retina (spot size) and the extent of retinal injury from single ultrashort laser pulses. From previous studies it is believed that the retinal effect of single 3-ps laser pulses should vary in extent and location, depending on the occurrence of laser-induce...

journal_title：Journal of biomedical optics

authors： Roach WP,Cain CP,Narayan DG,Noojin GD,Boppart SA,Birngruber R,Fujimoto JG,Toth CA

更新日期：2004-11-01 00:00:00

abstract：:We present in vivo human total retinal blood flow measurements using Doppler Fourier domain optical coherence tomography (OCT). The scan pattern consisted of two concentric circles around the optic nerve head, transecting all retinal branch arteries and veins. The relative positions of each blood vessel in the two OCT...

journal_title：Journal of biomedical optics

authors： Wang Y,Bower BA,Izatt JA,Tan O,Huang D

更新日期：2008-11-01 00:00:00

abstract：:Two-photon excitation microscopy is one of the key techniques used to observe three-dimensional (3-D) structures in biological samples. We utilized a visible-wavelength laser beam for two-photon excitation in a multifocus confocal scanning system to improve the spatial resolution and image contrast in 3-D live-cell im...

journal_title：Journal of biomedical optics

authors： Oketani R,Suda H,Uegaki K,Kubo T,Matsuda T,Yamanaka M,Arai Y,Smith N,Nagai T,Fujita K

更新日期：2019-11-01 00:00:00

abstract：:A new, simple, high-speed, and hardware-only integration-based fluorescence-lifetime-sensing algorithm using a center-of-mass method (CMM) is proposed to implement lifetime calculations, and its signal-to-noise-ratio based on statistics theory is also deduced. Compared to the commonly used iterative least-squares meth...

journal_title：Journal of biomedical optics

authors： Li DU,Rae B,Andrews R,Arlt J,Henderson R

更新日期：2010-01-01 00:00:00

abstract：:When irradiated with nanosecond laser pulses, gold nanoparticles allow for manipulation or destruction of cells and proteins with high spatial and temporal precision. Gold nanorods are especially attractive, because they have an up-to-20-fold stronger absorption than a sphere of equal volume, which is shifted to the o...

journal_title：Journal of biomedical optics

authors： Rudnitzki F,Bever M,Rahmanzadeh R,Brieger K,Endl E,Groll J,Hüttmann G

更新日期：2012-05-01 00:00:00

abstract：PURPOSE:Investigations of foveal aberrations assume circular pupils. However, the pupil becomes increasingly elliptical with increase in visual field eccentricity. We address this and other issues concerning peripheral aberration specification. METHODS:One approach uses an elliptical pupil similar to the actual pupil ...

journal_title：Journal of biomedical optics

authors： Charman WN,Mathur A,Scott DH,Hartwig A,Atchison DA

更新日期：2012-02-01 00:00:00

abstract：:The specificity of the hemodynamic response function (HRF) is determined spatially by the vascular architecture and temporally by the evolution of hemodynamic changes. Here, we used functional photoacoustic microscopy (fPAM) to investigate single cerebral blood vessels of rats after left forepaw stimulation. In this s...

journal_title：Journal of biomedical optics

authors： Liao LD,Lin CT,Shih YY,Lai HY,Zhao WT,Duong TQ,Chang JY,Chen YY,Li ML

更新日期：2012-06-01 00:00:00

abstract：:Radiotherapy is one of the main methods to treat cancer. However, due to the propagation pattern of high-energy photons in tissue and their inability to discriminate between healthy and malignant tissues, healthy tissues may also be damaged, causing undesired side effects. A possible method for internal electron thera...

journal_title：Journal of biomedical optics

authors： Tepper M,Barkai U,Gannot I

更新日期：2015-05-01 00:00:00

abstract：:In order to understand the relationship between accommodation and vision quality, a custom-built ultra-long scan depth spectral domain optical coherence tomography (UL-SDOCT) and a Shack-Hartmann wavefront sensor (HSWFS) were combined. The resolution and scan depth of UL-SDOCT are 6 μm and 15.6 mm, respectively, which...

journal_title：Journal of biomedical optics

authors： Shi G,Wang Y,Yuan Y,Wei L,Lv F,Zhang Y

更新日期：2012-12-01 00:00:00