Quantification of endocytosis using a folate functionalized silica hollow nanoshell platform.

Abstract:

:A quantification method to measure endocytosis was designed to assess cellular uptake and specificity of a targeting nanoparticle platform. A simple N -hydroxysuccinimide ester conjugation technique to functionalize 100-nm hollow silica nanoshell particles with fluorescent reporter fluorescein isothiocyanate and folate or polyethylene glycol (PEG) was developed. Functionalized nanoshells were characterized using scanning electron microscopy and transmission electron microscopy and the maximum amount of folate functionalized on nanoshell surfaces was quantified with UV-Vis spectroscopy. The extent of endocytosis by HeLa cervical cancer cells and human foreskin fibroblast (HFF-1) cells was investigated in vitro using fluorescence and confocal microscopy. A simple fluorescence ratio analysis was developed to quantify endocytosis versus surface adhesion. Nanoshells functionalized with folate showed enhanced endocytosis by cancer cells when compared to PEG functionalized nanoshells. Fluorescence ratio analyses showed that 95% of folate functionalized silica nanoshells which adhered to cancer cells were endocytosed, while only 27% of PEG functionalized nanoshells adhered to the cell surface and underwent endocytosis when functionalized with 200 and 900  μg , respectively. Additionally, the endocytosis of folate functionalized nanoshells proved to be cancer cell selective while sparing normal cells. The developed fluorescence ratio analysis is a simple and rapid verification/validation method to quantify cellular uptake between datasets by using an internal control for normalization.

journal_name

J Biomed Opt

authors

Sandoval S,Mendez N,Alfaro JG,Yang J,Aschemeyer S,Liberman A,Trogler WC,Kummel AC

doi

10.1117/1.JBO.20.8.088003

subject

Has Abstract

pub_date

2015-08-01 00:00:00

pages

88003

issue

8

eissn

1083-3668

issn

1560-2281

pii

2432770

journal_volume

20

pub_type

杂志文章
  • Study of molecule variations in renal tumor based on confocal micro-Raman spectroscopy.

    abstract::Confocal micro-Raman spectroscopy--a valuable analytical tool in biological and medical field of research-allows probing molecular vibrations of samples without external labels or extensive preparation. We employ confocal micro-Raman spectroscopy to characterize renal tumors and normal tissue. Results show that Raman ...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.JBO.18.3.031103

    authors: Zhuang Z,Li N,Guo Z,Zhu M,Xiong K,Chen S

    更新日期:2013-03-01 00:00:00

  • Image reconstruction in photoacoustic tomography with variable speed of sound using a higher-order geometrical acoustics approximation.

    abstract::Previous research correcting for variable speed of sound in photoacoustic tomography (PAT) based on a generalized radon transform (GRT) model assumes first-order geometrical acoustics (GA) approximation. In the GRT model, the pressure is related to the optical absorption, in an acoustically inhomogeneous medium, throu...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.3333550

    authors: Modgil D,Anastasio MA,La Rivière PJ

    更新日期:2010-03-01 00:00:00

  • Overview of diffuse optical tomography and its clinical applications.

    abstract::Near-infrared diffuse optical tomography (DOT), one of the most sophisticated optical imaging techniques for observations through biological tissue, allows 3-D quantitative imaging of optical properties, which include functional and anatomical information. With DOT, it is expected to be possible to overcome the limita...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章,评审

    doi:10.1117/1.JBO.21.9.091312

    authors: Hoshi Y,Yamada Y

    更新日期:2016-09-01 00:00:00

  • Measurement of optical path length change following pulsed laser irradiation using differential phase optical coherence tomography.

    abstract::Differential phase optical coherence tomography (DPOCT) is introduced to measure optical path length changes in response to pulsed laser irradiation (585 nm). An analytical equation that includes thermoelastic surface displacement and thermorefractive index change is derived to predict optical path length change in re...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.2236289

    authors: Kim J,Oh J,Milner TE

    更新日期:2006-07-01 00:00:00

  • Low-level chemiluminescent analysis of nondiluted human blood reveals its dynamic system properties.

    abstract::Lucigenin- and luminol-dependent chemiluminescence [(LC-CL) and (LM-CL)] in nondiluted human blood was studied. LM-CL was low in fresh blood and disappeared after its storage for 3 h, though the respiratory burst (RB) stimulation in blood was followed by high intensity and long-lasting LM-CL. LC-CL was high in fresh b...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.429921

    authors: Voeikov VL,Novikov CN,Vilenskaya ND

    更新日期:1999-01-01 00:00:00

  • Hyperspectral multiplex single-particle tracking of different receptor subtypes labeled with quantum dots in live neurons.

    abstract::The efficacy of existing therapies and the discovery of innovative treatments for central nervous system (CNS) diseases have been limited by the lack of appropriate methods to investigate complex molecular processes at the synaptic level. To improve our capability to investigate complex mechanisms of synaptic signalin...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.JBO.21.4.046008

    authors: Labrecque S,Sylvestre JP,Marcet S,Mangiarini F,Bourgoin B,Verhaegen M,Blais-Ouellette S,De Koninck P

    更新日期:2016-04-30 00:00:00

  • Specific absorption spectra of hemoglobin at different PO2 levels: potential noninvasive method to detect PO2 in tissues.

    abstract::Hemoglobin (Hb), as one of main components of blood, has a unique quaternary structure. Its release of oxygen is controlled by oxygen partial pressure (PO2). We investigate the specific spectroscopic changes in Hb under different PO2 levels to optimize clinical methods of measuring tissue PO2. The transmissivity of Hb...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.JBO.17.12.125002

    authors: Liu P,Zhu Z,Zeng C,Nie G

    更新日期:2012-12-01 00:00:00

  • SpineLab: tool for three-dimensional reconstruction of neuronal cell morphology.

    abstract::SpineLab is a software tool developed for reconstructing neuronal feature skeletons from three-dimensional single- or multi-photon image stacks. These images often suffer from limited resolution and a low signal-to-noise ratio, making the extraction of morphometric information difficult. To overcome this limitation, w...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.JBO.17.7.076007

    authors: Jungblut D,Vlachos A,Schuldt G,Zahn N,Deller T,Wittum G

    更新日期:2012-07-01 00:00:00

  • Analysis of the metabolic deterioration of ex vivo skin from ischemic necrosis through the imaging of intracellular NAD(P)H by multiphoton tomography and fluorescence lifetime imaging microscopy.

    abstract::Ex vivo human skin has been used extensively for cosmeceutical and drug delivery studies, transplantable skin allografts, or skin flaps. However, it has a half-life of a few days due to ischemic necrosis. Traditional methods of assessing viability can be time-consuming and provide limited metabolic information. Using ...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.3466580

    authors: Sanchez WY,Prow TW,Sanchez WH,Grice JE,Roberts MS

    更新日期:2010-07-01 00:00:00

  • Hyperspectral wide gap second derivative analysis for in vivo detection of cervical intraepithelial neoplasia.

    abstract::Hyperspectral reflectance imaging technique has been used for in vivo detection of cervical intraepithelial neoplasia. However, the clinical outcome of this technique is suboptimal owing to multiple limitations such as nonuniform illumination, high-cost and bulky setup, and time-consuming data acquisition and processi...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.JBO.20.12.121303

    authors: Zheng W,Wang C,Chang S,Zhang S,Xu RX

    更新日期:2015-12-01 00:00:00

  • Determination of optical properties of human blood in the spectral range 250 to 1100 nm using Monte Carlo simulations with hematocrit-dependent effective scattering phase functions.

    abstract::The absorption coefficient mu(a), scattering coefficient mu(s), and anisotropy factor g of diluted and undiluted human blood (hematocrit 0.84 and 42.1%) are determined under flow conditions in the wavelength range 250 to 1100 nm, covering the absorption bands of hemoglobin. These values are obtained by high precision ...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.2203659

    authors: Friebel M,Roggan A,Müller G,Meinke M

    更新日期:2006-05-01 00:00:00

  • Compact instrument for fluorescence image-guided surgery.

    abstract::Fluorescence image-guided surgery (FIGS) is an emerging technique in oncology, neurology, and cardiology. To adapt intraoperative imaging for various surgical applications, increasingly flexible and compact FIGS instruments are necessary. We present a compact, portable FIGS system and demonstrate its use in cardiovasc...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.3378128

    authors: Wang X,Bhaumik S,Li Q,Staudinger VP,Yazdanfar S

    更新日期:2010-03-01 00:00:00

  • In vivo spectral imaging of different cell types in the small intestine by two-photon excited autofluorescence.

    abstract::Spectrally resolved two-photon excited autofluorescence imaging is used to distinguish different cell types and functional areas during dynamic processes in the living gut. Excitation and emission spectra of mucosal tissue and tissue components are correlated to spectra of endogenous chromophores. We show that selecti...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.3655587

    authors: Orzekowsky-Schroeder R,Klinger A,Martensen B,Blessenohl M,Gebert A,Vogel A,Hüttmann G

    更新日期:2011-11-01 00:00:00

  • Label-free optical activation of astrocyte in vivo.

    abstract::As the most abundant cell type in the central nervous system, astrocyte has been one of main research topics in neuroscience. Although various tools have been developed, at present, there is no tool that allows noninvasive activation of astrocyte in vivo without genetic or pharmacological perturbation. Here we report ...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.3600774

    authors: Choi M,Yoon J,Ku T,Choi K,Choi C

    更新日期:2011-07-01 00:00:00

  • Theory and practical recommendations for autocorrelation-based image correlation spectroscopy.

    abstract::Image correlation spectroscopy (ICS) is a powerful technique for detecting arrangement of fluorophores in images. This tutorial gives background into the mathematical underpinnings of ICS, specifically image autocorrelation. The effects of various artifacts and image processing steps, including background subtraction,...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.JBO.17.8.080801

    authors: Robertson C,George SC

    更新日期:2012-08-01 00:00:00

  • "Seeing" electroencephalogram through the skull: imaging prefrontal cortex with fast optical signal.

    abstract::Near-infrared spectroscopy is a novel imaging technique potentially sensitive to both brain hemodynamics (slow signal) and neuronal activity (fast optical signal, FOS). The big challenge of measuring FOS noninvasively lies in the presumably low signal-to-noise ratio. Thus, detectability of the FOS has been controversi...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.3505007

    authors: Medvedev AV,Kainerstorfer JM,Borisov SV,Gandjbakhche AH,Vanmeter J

    更新日期:2010-11-01 00:00:00

  • Combined ultrasound and photoacoustic imaging to detect and stage deep vein thrombosis: phantom and ex vivo studies.

    abstract::Treatment of deep venous thrombosis (DVT)--a primary cause of potentially fatal pulmonary embolism (PE)--depends on the age of the thrombus. The existing clinical imaging methods are capable of visualizing a thrombus but cannot determine the age of the blood clot. Therefore, there is a need for an imaging technique to...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.2992175

    authors: Karpiouk AB,Aglyamov SR,Mallidi S,Shah J,Scott WG,Rubin JM,Emelianov SY

    更新日期:2008-09-01 00:00:00

  • Correlation of in vivo photosensitizer fluorescence and photodynamic-therapy-induced depth of necrosis in a murine tumor model.

    abstract::We compared light-induced fluorescence (LIF) to nominal injected drug dose for predicting the depth of necrosis response to photodynamic therapy (PDT) in a murine tumor model. Mice were implanted with radiation-induced fibrosarcoma (RIF) and were injected with 0, 5, or 10 mg/kg Photofrin. 630-nm light (30 J/cm(2), 75 ...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.1560011

    authors: Cheung R,Solonenko M,Busch TM,Del Piero F,Putt ME,Hahn SM,Yodh AG

    更新日期:2003-04-01 00:00:00

  • Handheld projective imaging device for near-infrared fluorescence imaging and intraoperative guidance of sentinel lymph node resection.

    abstract::We propose a handheld projective imaging device for orthotopic projection of near-infrared fluorescence images onto target biological tissue at visible wavelengths without any additional visual aid. The device integrates a laser diode light source module, a camera module, a projector, an ultrasonic distance sensor, a ...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.JBO.24.8.080503

    authors: Li C,Liu P,Shao P,Pei J,Li Y,Pawlik TM,Martin EW,Xu RX

    更新日期:2019-08-01 00:00:00

  • Measurement of oxygen saturation in small retinal vessels with adaptive optics confocal scanning laser ophthalmoscope.

    abstract::We have used an adaptive optics confocal scanning laser ophthalmoscope to assess oxygen saturation in small retinal vessels. Images of the vessels with a diameter smaller than 50 μm are recorded at oxygen sensitive and isosbestic wavelengths (680 and 796 nm, respectively). The vessel optical densities (ODs) are determ...

    journal_title:Journal of biomedical optics

    pub_type: 信件

    doi:10.1117/1.3655354

    authors: Li H,Lu J,Shi G,Zhang Y

    更新日期:2011-11-01 00:00:00

  • Quantitative concentration measurements of creatinine dissolved in water and urine using Raman spectroscopy and a liquid core optical fiber.

    abstract::We describe the use of a Teflon-AF liquid core optical fiber (LCOF) geometry to enhance the collection of Raman scattering from the biochemical creatinine, dissolved in water and in urine. At short integration times, where shot noise is most troublesome, the enhanced signal leads to greater accuracy in estimating the ...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.1917842

    authors: Qi D,Berger AJ

    更新日期:2005-05-01 00:00:00

  • Computational segmentation of collagen fibers from second-harmonic generation images of breast cancer.

    abstract::Second-harmonic generation (SHG) imaging can help reveal interactions between collagen fibers and cancer cells. Quantitative analysis of SHG images of collagen fibers is challenged by the heterogeneity of collagen structures and low signal-to-noise ratio often found while imaging collagen in tissue. The role of collag...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.JBO.19.1.016007

    authors: Bredfeldt JS,Liu Y,Pehlke CA,Conklin MW,Szulczewski JM,Inman DR,Keely PJ,Nowak RD,Mackie TR,Eliceiri KW

    更新日期:2014-01-01 00:00:00

  • Reproducibility of parameters of postocclusive reactive hyperemia measured by diffuse optical tomography.

    abstract::The application of near-infrared spectroscopy (NIRS) to assess microvascular function has shown promising results. An important limitation when using a single source-detector pair, however, is the lack of depth sensitivity. Diffuse optical tomography (DOT) overcomes this limitation using an array of sources and detect...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.JBO.21.6.066012

    authors: Vidal-Rosas EE,Billings SA,Chico T,Coca D

    更新日期:2016-06-01 00:00:00

  • In-vivo Fourier domain optical coherence tomography as a new tool for investigation of vasodynamics in the mouse model.

    abstract::In-vivo imaging of the vascular system can provide novel insight into the dynamics of vasoconstriction and vasodilation. Fourier domain optical coherence tomography (FD-OCT) is an optical, noncontact imaging technique based on interferometry of short-coherent near-infrared light with axial resolution of less than 10 m...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.3149865

    authors: Meissner S,Müller G,Walther J,Morawietz H,Koch E

    更新日期:2009-05-01 00:00:00

  • Photochemical effects and hypericin photosensitized processes in collagen.

    abstract::Emission and excitation spectra of collagen were recorded in the ultraviolet and visible regions. The existence of several types of chromophores absorbing and emitting throughout these spectral regions was observed. It was shown that laser irradiation at 355 and 532 nm caused collagen fluorescence photobleaching by 30...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.1331559

    authors: Yova D,Hovhannisyan V,Theodossiou T

    更新日期:2001-01-01 00:00:00

  • Visible-wavelength two-photon excitation microscopy with multifocus scanning for volumetric live-cell imaging.

    abstract::Two-photon excitation microscopy is one of the key techniques used to observe three-dimensional (3-D) structures in biological samples. We utilized a visible-wavelength laser beam for two-photon excitation in a multifocus confocal scanning system to improve the spatial resolution and image contrast in 3-D live-cell im...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.JBO.25.1.014502

    authors: Oketani R,Suda H,Uegaki K,Kubo T,Matsuda T,Yamanaka M,Arai Y,Smith N,Nagai T,Fujita K

    更新日期:2019-11-01 00:00:00

  • Optically tunable nanoparticle contrast agents for early cancer detection: model-based analysis of gold nanoshells.

    abstract::Many optical diagnostic approaches rely on changes in scattering and absorption properties to generate optical contrast between normal and diseased tissue. Recently, there has been increasing interest in using exogenous agents to enhance this intrinsic contrast with particular emphasis on the development for targeting...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.2141825

    authors: Lin AW,Lewinski NA,West JL,Halas NJ,Drezek RA

    更新日期:2005-11-01 00:00:00

  • Ultrafast optical property map generation using lookup tables.

    abstract::Imaging technologies working in the spatial frequency domain are becoming increasingly popular for generating wide-field maps of optical properties, enabling rapid analysis of tissue parameters. While acquisition methods have become faster and are now performing in real-time, processing methods remain slow, precluding...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.JBO.21.11.110501

    authors: Angelo J,Vargas CR,Lee BT,Bigio IJ,Gioux S

    更新日期:2016-11-01 00:00:00

  • In vivo super-resolution retinal imaging through virtually structured detection.

    abstract::High resolution is important for sensitive detection of subtle distortions of retinal morphology at an early stage of eye diseases. We demonstrate virtually structured detection (VSD) as a feasible method to achieve in vivo super-resolution ophthalmoscopy. A line-scanning strategy was employed to achieve a super-resol...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.JBO.21.12.120502

    authors: Liu C,Zhi Y,Wang B,Thapa D,Chen Y,Alam M,Lu Y,Yao X

    更新日期:2016-12-01 00:00:00

  • Two-photon microscopy on vital carotid arteries: imaging the relationship between collagen and inflammatory cells in atherosclerotic plaques.

    abstract::We used two-photon laser scanning microscopy (TPLSM) to demonstrate for the first time its potential in studying relational details at the cellular level of atherogenesis in intact, viable mouse carotid arteries. Isolated and mounted arteries of ApoE-/-mice, aged 15 or 21 weeks (7 and 13 weeks on western diet), were i...

    journal_title:Journal of biomedical optics

    pub_type: 杂志文章

    doi:10.1117/1.2965542

    authors: Megens RT,oude Egbrink MG,Merkx M,Slaaf DW,van Zandvoort MA

    更新日期:2008-07-01 00:00:00