Abstract:
:We compared light-induced fluorescence (LIF) to nominal injected drug dose for predicting the depth of necrosis response to photodynamic therapy (PDT) in a murine tumor model. Mice were implanted with radiation-induced fibrosarcoma (RIF) and were injected with 0, 5, or 10 mg/kg Photofrin. 630-nm light (30 J/cm(2), 75 mW/cm(2)) was delivered to the tumor after 24 hours. Fluorescence emission (lambda(excitation)=545 nm, lambda( emission)=628 nm) from the tumor was measured. The LIF data had less scatter than injected drug dose, and was found to be at least as good as an injected drug dose for predicting the depth of necrosis after PDT. Our observations provide further evidence that fluorescence spectroscopy can be used to quantify tissue photosensitizer uptake and to predict PDT tissue damage.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Cheung R,Solonenko M,Busch TM,Del Piero F,Putt ME,Hahn SM,Yodh AGdoi
10.1117/1.1560011subject
Has Abstractpub_date
2003-04-01 00:00:00pages
248-52issue
2eissn
1083-3668issn
1560-2281journal_volume
8pub_type
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