Abstract:
:Vascular leakage is an important feature of various disease conditions. In vivo optical imaging provides a great opportunity for the evaluation of this phenomenon. In the present study, we focus on the development and validation of a near-infrared (NIR) imaging formula to allow reliable, cost-efficient evaluation of vascular leakage in diverse species using the existing small-animal fluorescence imaging technology. IR-676, a moderately hydrophobic NIR cyanine dye, was doped into self-assembling aqueous micelles using a widely employed and safe nonionic emulsifier (Kolliphor HS 15), and was tested in several acute and chronic inflammatory disease models in both mice and rats. The imaging formula is stable and exerts no acute toxic effects in vitro. It accumulated specifically in the inflamed regions in all models, which could be demonstrated by both conventional epifluorescence imaging, and fluorescence tomography both as a standalone technique and also by merging it with computed tomography scans. Ex vivo verification of dye accumulation by confocal fluorescence microscopy was also possible. The present formula allows sensitive and specific detection of inflammatory plasma leakage in diverse models. Its potential for imaging larger animals was also demonstrated. IR-676-doped micelles offer an excellent opportunity to image inflammatory vascular leakage in various models and species.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Botz B,Bölcskei K,Kemény Á,Sándor Z,Tékus V,Sétáló G Jr,Csepregi J,Mócsai A,Pintér E,Kollár L,Helyes Zdoi
10.1117/1.JBO.20.1.016022subject
Has Abstractpub_date
2015-01-01 00:00:00pages
016022issue
1eissn
1083-3668issn
1560-2281pii
2108060journal_volume
20pub_type
杂志文章abstract::Intravascular near-infrared fluorescence (NIRF) imaging offers a new approach for characterizing atherosclerotic plaque, but random catheter positioning within the vessel lumen results in variable light attenuation and can yield inaccurate measurements. We hypothesized that NIRF measurements could be corrected for var...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.5.056009
更新日期:2013-05-01 00:00:00
abstract::A photoacoustic imaging system that incorporates a commercial ultrasonic camera for real-time imaging of two-dimensional (2-D) projection planes in tissue at video rate (30 Hz) is presented. The system uses a Q-switched frequency-doubled Nd:YAG pulsed laser for photoacoustic generation. The ultrasonic camera consists ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3420079
更新日期:2010-03-01 00:00:00
abstract::Complete and continuous imaging of microvascular networks is crucial for a wide variety of biomedical applications. Photoacoustic tomography can provide high resolution microvascular imaging using hemoglobin within red blood cells (RBCs) as an endogenic contrast agent. However, intermittent RBC flow in capillaries res...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3251044
更新日期:2009-09-01 00:00:00
abstract::We experimentally study potential mechanisms by which the enhancement factor in low-coherence enhanced backscattering (LEBS) can probe subtle variations in radial intensity distribution in weakly scattering media. We use enhanced backscattering of light by implementing either (1) low spatial coherence illumination or ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3443795
更新日期:2010-05-01 00:00:00
abstract::An algorithm was developed to obtain depth-resolved local optical axis in birefringent samples by using conventional polarization-sensitive optical coherence tomography (PSOCT) that uses a single circularly polarized incident light. The round-trip sample Jones matrices were first constructed from the cumulative PSOCT ...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.JBO.17.11.110501
更新日期:2012-11-01 00:00:00
abstract::We present the application of a microfluidic optical cell stretcher to measure the elasticity of malaria-infected red blood cells. The measurements confirm an increase in host cell rigidity during the maturation of the parasite Plasmodium falciparum. The device combines the selectivity and sensitivity of single-cell e...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3458919
更新日期:2010-05-01 00:00:00
abstract::The spatiotemporal characteristics of changes in cerebral blood volume associated with neuronal activity were investigated in the hindlimb somatosensory cortex of alpha-chloralose-urethane anesthetized rats (n=10) with optical imaging at 570 nm through a thinned skull. Activation of the cortex was carried out by elect...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1609199
更新日期:2003-10-01 00:00:00
abstract::We have employed near-infrared optical methods to measure noninvasively the dynamics of muscle blood flow and oxygen saturation (StO2) during cuff occlusion and plantar flexion exercise. Relative muscle oxygen consumption (rVO2) was also computed from these data. Diffuse correlation spectroscopy provides information a...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1884603
更新日期:2005-03-01 00:00:00
abstract::Application of a fiber optic biosensor (FOB) to the real-time investigation of the interaction kinetics between FITC-conjugated monoclonal sheep anti-human C-reactive protein (CRP) antibody and CRP isoforms on the surface of optical fiber is described. Recently, both the native pentameric CRP (pCRP), an acute phase pr...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2714029
更新日期:2007-03-01 00:00:00
abstract::Hyperspectral reflectance imaging technique has been used for in vivo detection of cervical intraepithelial neoplasia. However, the clinical outcome of this technique is suboptimal owing to multiple limitations such as nonuniform illumination, high-cost and bulky setup, and time-consuming data acquisition and processi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.12.121303
更新日期:2015-12-01 00:00:00
abstract::The present work describes the fabrication and characterization of an optical fiber pH sensor using a sol–gel technique. The sensing head configuration is incorporated using a short section of no-core fiber, coated with tetraethyl orthosilicate and spliced at the end of a single mode fiber with a bulge. Different type...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.5.057001
更新日期:2017-05-01 00:00:00
abstract::Sinus blockages are a common reason for physician visits, affecting one out of seven people in the United States, and often require medical treatment. Diagnosis in the primary care setting is challenging because symptom criteria (via detailed clinical history) plus objective imaging [computed tomography (CT) or endosc...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.3.035004
更新日期:2015-03-01 00:00:00
abstract::Microvascular network of the retina plays an important role in diagnosis and monitoring of various retinal diseases. We propose a three-dimensional (3-D) segmentation method with intensity-based Doppler variance (IBDV) based on swept-source optical coherence tomography. The automatic 3-D segmentation method is used to...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.7.076003
更新日期:2015-07-01 00:00:00
abstract::We present for the first time in vivo full three-dimensional (3-D) photoacoustic tomography (PAT) of the distal interphalangeal joint in a human subject. Both absorbed energy density and absorption coefficient images of the joint are quantitatively obtained using our finite-element-based photoacoustic image reconstruc...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3257246
更新日期:2009-11-01 00:00:00
abstract::Selective photothermal interaction using dye enhancement has proven to be effective in minimizing surrounding tissue damage and delivering energy to target tissue. During laser irradiation, the process of photon absorption and thermal energy diffusion in the target tissue and its surrounding tissue are crucial. Such i...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2204615
更新日期:2006-05-01 00:00:00
abstract::A swept source (SS)-based circular-state (CS) polarization-sensitive optical coherence tomography (PS-OCT) constructed entirely with polarization-maintaining fiber optics components is proposed with the experimental verification. By means of the proposed calibration scheme, bulk quarter-wave plates can be replaced by ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.2.021110
更新日期:2014-02-01 00:00:00
abstract::The effect of glucose on fluorescence of synthesized ZnCdS nanoparticles in the presence of glucose oxidase or in a mixture of glucose oxidase and peroxidase has been investigated. Behavior of fluorescence characteristics of ZnCdS nanoparticles with nonstabilized surface and coated with polymer shell is compared. It h...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.1.011020
更新日期:2014-01-01 00:00:00
abstract::In order to understand the relationship between accommodation and vision quality, a custom-built ultra-long scan depth spectral domain optical coherence tomography (UL-SDOCT) and a Shack-Hartmann wavefront sensor (HSWFS) were combined. The resolution and scan depth of UL-SDOCT are 6 μm and 15.6 mm, respectively, which...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.JBO.17.12.120501
更新日期:2012-12-01 00:00:00
abstract::Many optical diagnostic approaches rely on changes in scattering and absorption properties to generate optical contrast between normal and diseased tissue. Recently, there has been increasing interest in using exogenous agents to enhance this intrinsic contrast with particular emphasis on the development for targeting...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2141825
更新日期:2005-11-01 00:00:00
abstract::We propose a useful method to boost the imaging speed for spectral domain optical coherence tomography (SDOCT) by multiplying a number of high-speed spectrometers used in the system with selective precise control of data-recording and data-reading phases for spectral cameras employed in each spectrometer. To demonstra...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3592492
更新日期:2011-06-01 00:00:00
abstract::We describe a computer eye model that allows for aspheric surfaces and a three-dimensional computer-based ray-tracing technique to simulate optical properties of the human eye and visual perception under various eye defects. Eye surfaces, such as the cornea, eye lens, and retina, are modeled or approximated by a set o...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2357734
更新日期:2006-09-01 00:00:00
abstract::Several studies demonstrated that the cyclic adenosine monophosphate (cAMP), an important second messenger, is involved in the mechanism of low-level laser irradiation (LLLI) treatment. However, most of these studies obtained the cAMP level in cell culture extracts or supernatant. In this study, the cAMP level in livi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.5.051029
更新日期:2015-05-01 00:00:00
abstract::We experimentally implement label-free phase microscopy using wide-field digital interferometry (WFDI) techniques to retrieve quantitative volumetric data of articular chondrocyte dynamics. Using the scanless interferometric system, we visualize chondrocyte swelling and bursting induced by hypo-osmotic pressure. Recon...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3290242
更新日期:2010-01-01 00:00:00
abstract::There are several pathologies whose study and diagnosis is impaired by a relatively small number of documented cases. A practical approach to overcome this obstacle and advance the research in this area consists in employing computer simulations to perform controlled in silico experiments. The results of these experim...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.9.097005
更新日期:2012-09-01 00:00:00
abstract::We present a method for tissue fluorescence quantification in situ using a handheld fiber optic probe that measures both the fluorescence and diffuse reflectance spectra. A simplified method to decouple the fluorescence spectrum from distorting effects of the tissue optical absorption and scattering is developed, with...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3523616
更新日期:2010-11-01 00:00:00
abstract::Current techniques to assess lymph node metastases in cancer patients include lymphoscintigraphy after administration of a nonspecific radiocolloid in order to locate and resect lymph nodes for pathological examination of harbored cancer cells. Clinical trials involving intradermal or subcutaneous injection of antibod...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.2953498
更新日期:2008-07-01 00:00:00
abstract::Functional near-infrared spectroscopy (fNIRS) is being increasingly applied to affective and social neuroscience research; however, the reliability of this method is still unclear. This study aimed to evaluate the test–retest reliability of the fNIRS-based prefrontal response to emotional stimuli. Twenty-six participa...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.1.016011
更新日期:2017-01-01 00:00:00
abstract::Time-deconvolution of the instrument response from fluorescence lifetime imaging microscopy (FLIM) data is usually necessary for accurate fluorescence lifetime estimation. In many applications, however, the instrument response is not available. In such cases, a blind deconvolution approach is required. An iterative me...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.7.075010
更新日期:2015-07-01 00:00:00
abstract::The intracellular refractive index is an important parameter that describes the optical density of the cytoplasm and the concentration of the intracellular solutes. The refractive index of adherently grown cells is difficult to access. We present a method in which silica microspheres in living cells are used to determ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.9.097001
更新日期:2012-09-01 00:00:00
abstract::The advantages of using a telecentric imaging system in digital holographic microscopy (DHM) to study biological specimens are highlighted. To this end, the performances of nontelecentric DHM and telecentric DHM are evaluated from the quantitative phase imaging (QPI) point of view. The evaluated stability of the micro...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.4.046022
更新日期:2014-04-01 00:00:00