Abstract:
:The advantages of using a telecentric imaging system in digital holographic microscopy (DHM) to study biological specimens are highlighted. To this end, the performances of nontelecentric DHM and telecentric DHM are evaluated from the quantitative phase imaging (QPI) point of view. The evaluated stability of the microscope allows single-shot QPI in DHM by using telecentric imaging systems. Quantitative phase maps of a section of the head of the drosophila melanogaster fly and of red blood cells are obtained via single-shot DHM with no numerical postprocessing. With these maps we show that the use of telecentric DHM provides larger field of view for a given magnification and permits more accurate QPI measurements with less number of computational operations.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Doblas A,Sánchez-Ortiga E,Martínez-Corral M,Saavedra G,Garcia-Sucerquia Jdoi
10.1117/1.JBO.19.4.046022subject
Has Abstractpub_date
2014-04-01 00:00:00pages
046022issue
4eissn
1083-3668issn
1560-2281pii
1867205journal_volume
19pub_type
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