Abstract:
:Our current research in the framework of an interdisciplinary project focuses on modelling the dynamics of the hemoglobin reoxygenation process in post-mortem human skin by reflectance spectrometry. The observations of reoxygenation of hemoglobin in livores after postmortem exposure to a cold environment relate the reoxygenation to the commonly known phenomenon that the color impression of livores changes from livid to pink under low ambient temperatures. We analyze the spectra with respect to a physical model describing the optical properties of human skin, discuss the dynamics of the reoxygenation, and propose a phenomenological model for reoxygenation. For additional characterization of the reflectance spectra, the curvature of the local minimum and maximum in the investigated spectral range is considered. There is a strong correlation between the curvature of specra at a wavelength of 560 nm and the concentration of O2-Hb. The analysis is carried out via C programs, as well as MySQL database queries in Java EE, JDBC, Matlab, and Python.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Belenki L,Sterzik V,Schulz K,Bohnert Mdoi
10.1117/1.JBO.18.1.017004subject
Has Abstractpub_date
2013-01-01 00:00:00pages
17004issue
1eissn
1083-3668issn
1560-2281pii
1556205journal_volume
18pub_type
杂志文章abstract::The present report addresses the application of surface plasmon resonance (SPR) phenomenon for urea sensing. The former promises a high sensitivity, label-free detection, and real-time information by monitoring the refractive index change at the metal–dielectric interface. In the present report, a highly sensitive ure...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.8.087006
更新日期:2016-08-01 00:00:00
abstract::We describe a systematic approach to image, track, and quantify the movements of HIV viruses embedded in human cervical mucus. The underlying motivation for this study is that, in HIV-infected adults, women account for more than half of all new cases and most of these women acquire the infection through heterosexual c...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.9.096001
更新日期:2016-09-01 00:00:00
abstract::Diffusion of therapeutic macromolecules through the extracellular matrix of tumor tissue is a crucial step in drug delivery. We use fluorescence correlation spectroscopy (FCS) to measure diffusion of IgG (150 kDa) and dextrans (155 kDa and 2 MDa) in solution, 5% gelatin hydrogel, and multicellular spheroids. Gel and s...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2982530
更新日期:2008-09-01 00:00:00
abstract::The refractive index of blood is a key biophysical parameter, which can reflect the physiological state. We measured the refractive index of whole blood and other components, such as serum, plasma, and hemoglobin, based on internal reflection by using a homemade apparatus in the spectral range of 400 to 750 nm. In add...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.24.3.035003
更新日期:2019-03-01 00:00:00
abstract::The intracellular refractive index is an important parameter that describes the optical density of the cytoplasm and the concentration of the intracellular solutes. The refractive index of adherently grown cells is difficult to access. We present a method in which silica microspheres in living cells are used to determ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.9.097001
更新日期:2012-09-01 00:00:00
abstract::Intraoperative fluorescence molecular imaging based on targeted fluorescence agents is an emerging approach to improve surgical and endoscopic imaging and guidance. Short exposure times per frame and implementation at video rates are necessary to provide continuous feedback to the physician and avoid motion artifacts....
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.4.046012
更新日期:2014-04-01 00:00:00
abstract::The nEUROPt protocol is one of two new protocols developed within the European project nEUROPt to characterize the performances of time-domain systems for optical imaging of the brain. It was applied in joint measurement campaigns to compare the various instruments and to assess the impact of technical improvements. T...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.8.086012
更新日期:2014-08-01 00:00:00
abstract::Invasive brain cancer cells cannot be visualized during surgery and so they are often not removed. These residual cancer cells give rise to recurrences. journal_title:Journal of biomedical optics pub_type: 杂志文章 doi:10.1117/1.JBO.21.9.094002 更新日期:2016-09-01 00:00:00
abstract::Raman spectroscopy (RS) has demonstrated great potential for in vivo cancer screening; however, the biophysical changes that occur for specific diagnoses remain unclear. We recently developed an inverse biophysical skin cancer model to address this issue. Here, we presented the first demonstration of in vivo melanoma ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.23.5.057002
更新日期:2018-05-01 00:00:00
abstract::The study presents comparison of near-infrared light propagation and near-infrared backscattered radiation power, as simulated with numerical modeling and measured live in a patient in clinical conditions with the use of the near-infrared transillumination-backscattering sounding (NIR-TBSS) technique. A unique chance ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2753756
更新日期:2007-07-01 00:00:00
abstract::Real-time medical imaging systems such as reflectance confocal microscopes and optical coherence microscopes are being tested in multiple-patient and multiple-center clinical trials. The modulation transfer function (MTF) of these systems at any given time influences the image information content and can affect the in...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2779352
更新日期:2007-09-01 00:00:00
abstract::As small animal optical imaging and tomography are gaining popularity for interrogating functional and molecular events in vivo, it becomes increasingly necessary to gain knowledge of the optical properties of the species investigated to better understand and describe photon propagation through their tissues. To achie...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2400702
更新日期:2006-11-01 00:00:00
abstract::Traditional white-light and fluorescent imaging techniques provide powerful methods to extract high-resolution information from two-dimensional (2-D) sections, but to retrieve information from a three-dimensional (3-D) volume they require relatively slow scanning methods that result in increased acquisition time. Usin...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.JBO.17.5.050505
更新日期:2012-05-01 00:00:00
abstract::Application of a fiber optic biosensor (FOB) to the real-time investigation of the interaction kinetics between FITC-conjugated monoclonal sheep anti-human C-reactive protein (CRP) antibody and CRP isoforms on the surface of optical fiber is described. Recently, both the native pentameric CRP (pCRP), an acute phase pr...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2714029
更新日期:2007-03-01 00:00:00
abstract::We present experiments and analyses of confocal reflectance and two-photon microscopy studies of zebra finch skull samples. The thin and hollow structure of these birds' skulls is quite translucent, which can allow in vivo transcranial two-photon imaging for brain activation monitoring. However, the skull structure is...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3155522
更新日期:2009-05-01 00:00:00
abstract::The transmembrane protein prestin is crucial to outer hair cell (OHC) electromotility and contributes to the sensitivity and frequency selectivity of mammalian hearing. The molecular mechanisms of electromotility remain unclear, but prestin is purported to function as both a voltage sensor and a molecular motor. Under...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2715202
更新日期:2007-03-01 00:00:00
abstract::In breast surgery, a lack of knowledge about what is below the tissue surface may lead to positive tumor margins and iatrogenic damage. Diffuse reflectance spectroscopy (DRS) is a spectroscopic technique that can distinguish between healthy and tumor tissue making it a suitable technology for intraoperative guidance. ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.23.11.115004
更新日期:2018-11-01 00:00:00
abstract::All aberrations produced inside a biospecimen can degrade the quality of a three-dimensional image in two-photon excitation laser scanning microscopy. Previously, we developed a transmissive liquid-crystal device to correct spherical aberrations that improved the image quality of a fixed-mouse-brain slice treated with...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.12.121503
更新日期:2016-12-01 00:00:00
abstract::Quantitative polarized light microscopy (qPLM) is a popular tool for the investigation of birefringent architectures in biological tissues. Collagen, the most abundant protein in mammals, is such a birefringent material. Interpretation of results of qPLM in terms of collagen network architecture and anisotropy is chal...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3241986
更新日期:2009-09-01 00:00:00
abstract::Heart disease is the primary cause of death in the western world. Many of these deaths are caused by the rupture of vulnerable plaque. Vulnerable plaques are characterized by a large lipid core covered by a thin fibrous cap. One method for detecting these plaques is reflection spectroscopy. Several studies have invest...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2186332
更新日期:2006-03-01 00:00:00
abstract::We examine the morphological and chemical changes in the pulp chamber dentin after using endodontic agents by scanning electron microscopy (SEM), Fourier transform Raman spectroscopy (FT-Raman), and micro energy-dispersive x-ray fluorescence spectrometry (μEDXRF). Thirty teeth were sectioned exposing the pulp chamber ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.7.075008
更新日期:2012-07-01 00:00:00
abstract::The properties of polarized light emerging from turbid media in the exact backscattering direction are studied by modulating the incident light polarization state and isolating the synchronous signal with lock-in amplifier detection. The results are reported for polystyrene microsphere suspensions in distilled water, ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.430004
更新日期:2000-07-01 00:00:00
abstract::Colorectal surgery is the standard treatment for patients with colorectal cancer. To overcome two of the main challenges, the circumferential resection margin and postoperative complications, real-time tissue assessment could be of great benefit during surgery. In this ex vivo study, diffuse reflectance spectroscopy (...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.10.106014
更新日期:2017-10-01 00:00:00
abstract::In the present applications of optical coherence tomography (OCT), parameters besides pure morphology are evaluated in skin tissue under in vivo conditions. Spatially mapped refractive indices and scattering coefficients may support tissue characterization for research and diagnostic purposes in cosmetics/pharmacy and...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.429972
更新日期:2000-01-01 00:00:00
abstract::The effect of sampling region size and tissue heterogeneity is examined using fluorescence histogram assessment in a rat prostate tumor model with benzoporphyrin derivative fluorophore. Spatial heterogeneity in the fluorescence signal occurs on both macroscopic and microscopic scales. The periphery of the tumor is mor...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2002978
更新日期:2005-07-01 00:00:00
abstract::SpineLab is a software tool developed for reconstructing neuronal feature skeletons from three-dimensional single- or multi-photon image stacks. These images often suffer from limited resolution and a low signal-to-noise ratio, making the extraction of morphometric information difficult. To overcome this limitation, w...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.7.076007
更新日期:2012-07-01 00:00:00
abstract::Extension of optical monitoring of intact tissues from the visible and ultraviolet to the near-infrared (NIR) range (700-1300 nm) was first undertaken in 1977 for the purpose of monitoring the redox behavior of Cytochrome c oxidase (cyt c ox) in vivo. Soon it became evident that the much greater NIR translucency of sk...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.429952
更新日期:1999-10-01 00:00:00
abstract::For clinical optoacoustic imaging, linear probes are preferably used because they allow versatile imaging of the human body with real-time display and free-hand probe guidance. The two-dimensional (2-D) optoacoustic image obtained with this type of probe is generally interpreted as a 2-D cross-section of the tissue ju...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.2.026011
更新日期:2013-02-01 00:00:00
abstract::High-speed (video-rate) fluorescence lifetime imaging (FLIM) through a flexible endoscope is reported based on gated optical image intensifier technology. The optimization and potential application of FLIM to tissue autofluorescence for clinical applications are discussed. ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2102807
更新日期:2005-09-01 00:00:00
abstract::We interrogate the ability of free-space fluorescence tomography to image small animals in vivo using charge-coupled device (CCD) camera measurements over 360-deg noncontact projections. We demonstrate the performance of normalized dual-wavelength measurements that are essential for in-vivo use, as they account for th...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3149854
更新日期:2009-05-01 00:00:00