Abstract:
:Diffusion of therapeutic macromolecules through the extracellular matrix of tumor tissue is a crucial step in drug delivery. We use fluorescence correlation spectroscopy (FCS) to measure diffusion of IgG (150 kDa) and dextrans (155 kDa and 2 MDa) in solution, 5% gelatin hydrogel, and multicellular spheroids. Gel and spheroids are used as model systems for the extracellular matrix. The diffusion depends on the complexity of the environment, as well as on the size and structural shape of the diffusing molecules. The results based on one-photon FCS are in good agreement with diffusion coefficients obtained with two-photon fluorescence recovery after photobleaching (FRAP) using the same microscope (Zeiss LSM510 META/Confocor2). However, FCS reveals anomalous or multicomponent diffusion in gel and spheroids, which are not resolvable with FRAP. This study demonstrates that one-photon FCS can be used to study the extracellular transport of macromolecules in tumor tissue, and that FCS provides additional information about diffusion properties compared to FRAP.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Reitan NK,Juthajan A,Lindmo T,de Lange Davies Cdoi
10.1117/1.2982530subject
Has Abstractpub_date
2008-09-01 00:00:00pages
054040issue
5eissn
1083-3668issn
1560-2281journal_volume
13pub_type
杂志文章abstract::A Monte Carlo algorithm is applied to simulate the measurements of a sample with glass slides sandwiched between the double integrating sphere (DIS) setup. The effects caused by various parameters, such as the sample port of integrating sphere, thicknesses, and optical properties of the sample, on light losses and opt...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2815691
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abstract::We experimentally compare the performance and image contrast of spontaneous Raman and coherent Stokes Raman scattering microscopy. We demonstrate the differences between these techniques on a series of geometry-controlled samples that range in complexity from a point (array of tips) to one-dimensional (line grating) a...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2016-12-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 信件
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更新日期:2018-05-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.1.017005
更新日期:2014-01-01 00:00:00
abstract::Noninvasive cardiovascular imaging could lead to the early detection and timely treatment of complex atherosclerotic lesions responsible for major cardiovascular events. Recent investigations have suggested that optical coherence tomography (OCT) is an ideal diagnostic tool due to the high resolution this technology a...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
abstract::We present in vivo human total retinal blood flow measurements using Doppler Fourier domain optical coherence tomography (OCT). The scan pattern consisted of two concentric circles around the optic nerve head, transecting all retinal branch arteries and veins. The relative positions of each blood vessel in the two OCT...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2008-11-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.12.121202
更新日期:2015-12-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.10.101502
更新日期:2014-01-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.7.077007
更新日期:2012-07-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2013-04-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2016-07-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.12.125005
更新日期:2015-01-01 00:00:00
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journal_title:Journal of biomedical optics
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更新日期:2016-10-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2016-08-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
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更新日期:2000-07-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.11.117003
更新日期:2014-01-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2012-12-01 00:00:00
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更新日期:2014-06-01 00:00:00
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journal_title:Journal of biomedical optics
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journal_title:Journal of biomedical optics
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更新日期:2013-02-01 00:00:00
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journal_title:Journal of biomedical optics
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journal_title:Journal of biomedical optics
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journal_title:Journal of biomedical optics
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2010-03-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2018-03-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2005-09-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2013-03-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2010-03-01 00:00:00