Abstract:
:We experimentally compare the performance and image contrast of spontaneous Raman and coherent Stokes Raman scattering microscopy. We demonstrate the differences between these techniques on a series of geometry-controlled samples that range in complexity from a point (array of tips) to one-dimensional (line grating) and, lastly, two-dimensional (checkereboard) microstructure. Through the use of this sample series, a comparison of the focal volume, achievable signal-to-noise, and resulting image contrast is made. The results demonstrate the efficiency and spatial resolution attainable in coherent Raman microscopy relative to spontaneous Raman microscopy. Additionally, we detail potential complications in the interpretation of coherent Raman images of sample fine structure, where contrast is no longer based solely on oscillator concentration but can be influenced by sample microstructure.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Kim H,Michaels CA,Bryant GW,Stranick SJdoi
10.1117/1.3533310subject
Has Abstractpub_date
2011-02-01 00:00:00pages
021107issue
2eissn
1083-3668issn
1560-2281journal_volume
16pub_type
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