Abstract:
:Using single-molecule microscopy, we present a method to quantify the number of single autofluorescent proteins when they cannot be optically resolved. This method relies on the measurement of the total intensity emitted by each aggregate until it photobleaches. This strategy overcomes the inherent problem of blinking of green fluorescent proteins. In the case of small protein aggregates, our method permits us to describe the mean composition with a precision of one protein. For aggregates containing a large number of proteins, it gives access to the average number of proteins gathered and a signature of the inhomogeneity of the aggregates' population. We applied this methodology to the quantification of small purified citrine multimers.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Cognet L,Tardin C,Négrier ML,Breillat C,Coussen F,Choquet D,Lounis Bdoi
10.1117/1.2940600subject
Has Abstractpub_date
2008-05-01 00:00:00pages
031216issue
3eissn
1083-3668issn
1560-2281journal_volume
13pub_type
杂志文章abstract::UV-induced collagen cross-linking is a promising treatment for keratoconus that stiffens corneal tissue and prevents further degeneration. Since keratoconus is generally localized, the efficacy of collagen cross-linking (CXL) treatments could be improved by stiffening only the weakened parts of the cornea. Here, we de...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.9.091504
更新日期:2017-09-01 00:00:00
abstract::Vascular leakage is an important feature of various disease conditions. In vivo optical imaging provides a great opportunity for the evaluation of this phenomenon. In the present study, we focus on the development and validation of a near-infrared (NIR) imaging formula to allow reliable, cost-efficient evaluation of v...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.1.016022
更新日期:2015-01-01 00:00:00
abstract::We demonstrate the ex vivo imaging of an oral cancerous sample with a swept-source optical coherence tomography (SS-OCT) system. With the axial resolution of 8 microm in free space and system sensitivity of 108 dB, we can well distinguish the normal and abnormal tissue portions in a sample. In particular, we analyze t...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2960632
更新日期:2008-07-01 00:00:00
abstract::A swept source (SS)-based circular-state (CS) polarization-sensitive optical coherence tomography (PS-OCT) constructed entirely with polarization-maintaining fiber optics components is proposed with the experimental verification. By means of the proposed calibration scheme, bulk quarter-wave plates can be replaced by ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.2.021110
更新日期:2014-02-01 00:00:00
abstract::Spectrally resolved two-photon excited autofluorescence imaging is used to distinguish different cell types and functional areas during dynamic processes in the living gut. Excitation and emission spectra of mucosal tissue and tissue components are correlated to spectra of endogenous chromophores. We show that selecti...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3655587
更新日期:2011-11-01 00:00:00
abstract::Optical-resolution photoacoustic microscopy (ORPAM) in theory provides lateral resolution equivalent to the optical diffraction limit. Scattering media, such as biological turbid media, attenuates the optical signal and also alters the diffraction-limited spot size of the focused beam. The ORPAM signal is generated on...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.12.121202
更新日期:2015-12-01 00:00:00
abstract::Oxygen plays a very important role in living cells. The intracellular level of oxygen is under tight control, as even a small deviation from normal oxygen level affects major cellular metabolic processes and is likely to result in cellular damage or cell death. This paper describes the use of the oxygen sensitive fluo...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1344190
更新日期:2001-04-01 00:00:00
abstract::The optical properties of biological tissues under stretching are investigated using a full-field ellipsometry technique based on a differential Mueller matrix formalism. Traditional photoelastic-based formalism for extracting the linear birefringence (LB) properties of stretched anisotropic optical samples ignores th...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.3.035006
更新日期:2017-03-01 00:00:00
abstract::The earliest steps of breast cancer begin with aberrations in mammary ductal structure. Techniques that enable an investigator to image in situ and then analyze the same tissue using biochemical tools facilitates identification of genetic networks and signaling pathways active in the imaged structure. Cellular confoca...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2065827
更新日期:2005-09-01 00:00:00
abstract::In laser retinal photocoagulation, short (<20 ms) pulses have been found to reduce thermal damage to the inner retina, decrease treatment time, and minimize pain. However, the safe therapeutic window (defined as the ratio of power for producing a rupture to that of mild coagulation) decreases with shorter exposures. T...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3130282
更新日期:2009-05-01 00:00:00
abstract::SpineLab is a software tool developed for reconstructing neuronal feature skeletons from three-dimensional single- or multi-photon image stacks. These images often suffer from limited resolution and a low signal-to-noise ratio, making the extraction of morphometric information difficult. To overcome this limitation, w...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.7.076007
更新日期:2012-07-01 00:00:00
abstract::A computer simulation called RHME (Retinal Heating in Moving Eye) is developed to simulate the heating pattern that occurs in the retina during a long-duration exposure to a continuous wave laser beam. The simulation takes into account eye movements that occur during a deliberate fixation. Due to the rapid (millisecon...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1783355
更新日期:2004-09-01 00:00:00
abstract::Quantitative polarized light microscopy (qPLM) is a popular tool for the investigation of birefringent architectures in biological tissues. Collagen, the most abundant protein in mammals, is such a birefringent material. Interpretation of results of qPLM in terms of collagen network architecture and anisotropy is chal...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3241986
更新日期:2009-09-01 00:00:00
abstract::Optical measurements of metabolism are ideally acquired in vivo; however, intravital measurements are often impractical. Accurate ex vivo assessments would greatly broaden the applicability of optical measurements of metabolism. We investigate the use of live tissue culture experiments to serve as a surrogate for in v...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.11.116015
更新日期:2012-11-01 00:00:00
abstract::We theoretically investigate the effect of noise on frequency-domain heterodyne and/or homodyne measurements of intensity-modulated beams propagating through diffusive media, such as a photon density wave. We assumed that the attenuated amplitude and delayed phase are estimated by taking the Fourier transform of the n...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.1.016010
更新日期:2012-01-01 00:00:00
abstract::By applying linear perturbation theory to the radiation transport equation, the inverse problem of optical diffusion tomography can be reduced to a set of linear equations, Wμ=R, where W is the weight function, μ are the cross-section perturbations to be imaged, and R is the detector readings perturbations. We have st...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.429870
更新日期:1998-04-01 00:00:00
abstract::This paper presents comprehensive and depth-resolved retinal microvasculature images within human retina achieved by a newly developed ultrahigh sensitive optical microangiography (UHS-OMAG) system. Due to its high flow sensitivity, UHS-OMAG is much more sensitive to tissue motion due to the involuntary movement of th...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3642638
更新日期:2011-10-01 00:00:00
abstract::Quantification of nicotinamide adenine dinucleotide (NADH) changes during functional brain activation and pathological conditions provides critical insight into brain metabolism. Of the different imaging modalities, two-photon laser scanning microscopy (TPLSM) is becoming an important tool for cellular-resolution meas...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3633339
更新日期:2011-10-01 00:00:00
abstract::We quantitatively investigated the measurement sensitivity of spatially resolved spectroscopy (SRS) across six tissue models: cerebral tissue, a small animal brain, the forehead of a fetus, an adult brain, forearm muscle, and thigh muscle. The optical path length in the voxel of the model was analyzed using Monte Carl...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.23.3.030503
更新日期:2018-03-01 00:00:00
abstract::Recent technological advancements in the photonics industry have led to a resurgence of interest in optical glucose sensing and to realistic progress toward the development of an optical glucose sensor. Such a sensor has the potential to significantly improve the quality of life for the estimated 16 million diabetics ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.429962
更新日期:2000-01-01 00:00:00
abstract::Lucigenin- and luminol-dependent chemiluminescence [(LC-CL) and (LM-CL)] in nondiluted human blood was studied. LM-CL was low in fresh blood and disappeared after its storage for 3 h, though the respiratory burst (RB) stimulation in blood was followed by high intensity and long-lasting LM-CL. LC-CL was high in fresh b...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.429921
更新日期:1999-01-01 00:00:00
abstract::Probing tissue with near-infrared radiation (NIR) simultaneously produces remitted fluorescence and Raman scattering (IE) plus Rayleigh∕Mie light scattering (EE) that noninvasively give chemical and physical information about the materials and objects within. We model tissue as a three-phase system: plasma and red blo...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3625283
更新日期:2011-09-01 00:00:00
abstract::Noninvasive cardiovascular imaging could lead to the early detection and timely treatment of complex atherosclerotic lesions responsible for major cardiovascular events. Recent investigations have suggested that optical coherence tomography (OCT) is an ideal diagnostic tool due to the high resolution this technology a...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2870153
更新日期:2008-01-01 00:00:00
abstract:UNLABELLED:Temperature changes caused by laser irradiation can promote damage to the surrounding dental tissues. In this study, we evaluated the temperature changes of recently extracted human mandibular incisors during intracanal irradiation with an 810-nm diode laser at different settings. Fifty mandibular incisors w...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.1.015006
更新日期:2012-01-01 00:00:00
abstract::A new, simple, high-speed, and hardware-only integration-based fluorescence-lifetime-sensing algorithm using a center-of-mass method (CMM) is proposed to implement lifetime calculations, and its signal-to-noise-ratio based on statistics theory is also deduced. Compared to the commonly used iterative least-squares meth...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3309737
更新日期:2010-01-01 00:00:00
abstract::Toward developing a new method for restoring tissue viscoelasticity in scarred vocal folds, we previously proposed a method to localize biomaterials within subepithelial voids ablated using ultrafast laser pulses. The clinical implementation of this method necessitates the quantification of the laser parameters for ab...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.11.118001
更新日期:2013-11-01 00:00:00
abstract::A real-time photoacoustic imaging system is designed and built. This system is based on a commercially available ultrasound imaging system. It can achieve a frame rate of 8 frames/sec. Vasculature in the hand of a human volunteer is imaged, and the resulting photoacoustic image is combined with the ultrasound image. T...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3005421
更新日期:2008-09-01 00:00:00
abstract::Nonviral, site-specific gene delivery to deep tissue is required for gene therapy of a spinal cord injury. However, an efficient method satisfying these requirements has not been established. This study demonstrates efficient and targeted gene transfer into the spinal cord by using photomechanical waves (PMWs), which ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3642014
更新日期:2011-10-01 00:00:00
abstract::Intraoperative fluorescence molecular imaging based on targeted fluorescence agents is an emerging approach to improve surgical and endoscopic imaging and guidance. Short exposure times per frame and implementation at video rates are necessary to provide continuous feedback to the physician and avoid motion artifacts....
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.4.046012
更新日期:2014-04-01 00:00:00
abstract::New diagnostic tools are needed for the characterization of dental caries in the early stages of development. If carious lesions are detected early enough, they can be arrested without the need for surgical intervention. The objective of this study was to demonstrate that polarization sensitive optical coherence tomog...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1509752
更新日期:2002-10-01 00:00:00