Abstract:
:Spectrally resolved two-photon excited autofluorescence imaging is used to distinguish different cell types and functional areas during dynamic processes in the living gut. Excitation and emission spectra of mucosal tissue and tissue components are correlated to spectra of endogenous chromophores. We show that selective excitation with only two different wavelengths within the tuning range of a Ti:sapphire femtosecond laser system yields excellent discrimination between enterocytes, antigen presenting cells and lysosomes based on the excitation and emission properties of their autofluorescence. The method is employed for time-lapse microscopy over up to 8 h. Changes of the spectral signature with the onset of photodamage are demonstrated, and their origin is discussed.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Orzekowsky-Schroeder R,Klinger A,Martensen B,Blessenohl M,Gebert A,Vogel A,Hüttmann Gdoi
10.1117/1.3655587subject
Has Abstractpub_date
2011-11-01 00:00:00pages
116025issue
11eissn
1083-3668issn
1560-2281journal_volume
16pub_type
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