Reflectance confocal microscopy for characterization of mammary ductal structures and development of neoplasia in genetically engineered mouse models of breast cancer.

abstract：:Protoporphyrin IX (PpIX)-encapsulated mesoporous silica nanoparticles were synthesized, characterized, and utilized for photodynamic therapy (PDT) of cancer. Silica encapsulation is relatively transparent for activated light and can protect the PpIX against denaturation induced by the extreme bioenvironment. The mesop...

journal_title：Journal of biomedical optics

authors： Qian J,Gharibi A,He S

更新日期：2009-01-01 00:00:00

abstract：:We present unsupervised clustering methods for automatic grouping of human red blood cells (RBCs) extracted from RBC quantitative phase images obtained by digital holographic microscopy into three RBC clusters with regular shapes, including biconcave, stomatocyte, and sphero-echinocyte. We select some good features re...

journal_title：Journal of biomedical optics

authors： Ahmadzadeh E,Jaferzadeh K,Lee J,Moon I

更新日期：2017-07-01 00:00:00

abstract：:We present for the first time in vivo full three-dimensional (3-D) photoacoustic tomography (PAT) of the distal interphalangeal joint in a human subject. Both absorbed energy density and absorption coefficient images of the joint are quantitatively obtained using our finite-element-based photoacoustic image reconstruc...

journal_title：Journal of biomedical optics

authors： Sun Y,Sobel E,Jiang H

更新日期：2009-11-01 00:00:00

abstract：:An approach to an established technique that is potentially applicable for a more comprehensive understanding of the electrical properties of red blood cells (RBCs) is presented. Using a high-intensity gradient laser trap, RBCs can be singly trapped and consequentially ionized. The subsequent dynamics of the ionized c...

journal_title：Journal of biomedical optics

authors： Kelley M,Cooper J,Devito D,Mushi R,Aguinaga MDP,Erenso DB

更新日期：2018-05-01 00:00:00

abstract：:Waveguide evanescent field fluorescence (WEFF) microscopy is a new development that allows the imaging of contact regions between biological cells and their substratum, as well as imaging of ultrathin films such as monomolecular Langmuir-Blodgett (LB) films. Mixed-lipid monolayer systems mimicking lung surfactant were...

journal_title：Journal of biomedical optics

authors： Hassanzadeh A,Mittler S

更新日期：2011-04-01 00:00:00

abstract：:Optical coherence tomography (OCT) provides more parameters than pure morphology does. In a recent paper [A. Knuettel and M. Boehlau-Godau, J. Biomed. Opt. 5(1) 83-92 (2000)] we have shown that the refractive index (RI) can be evaluated in a localized manner in skin tissue under in vivo conditions. Based on a theory, ...

journal_title：Journal of biomedical optics

authors： Knüttel A,Bonev S,Knaak W

更新日期：2004-03-01 00:00:00

abstract：:Pelvic organ prolapse (POP) occurs when changes to the pelvic organ support structures cause descent or herniation of the pelvic organs into the vagina. Clinical evaluation of POP is a series of manual measurements known as the pelvic organ prolapse quantification (POP-Q) score. However, it fails to identify the mecha...

journal_title：Journal of biomedical optics

authors： Parkinson LA,Gargett CE,Young N,Rosamilia A,Vashi AV,Werkmeister JA,Papageorgiou AW,Arkwright JW

更新日期：2016-12-01 00:00:00

abstract：:Optical clearing is a well-known phenomenon. It is based on the matching of refractive indices of a bulk material and scattering particles. The same principle is also used in scattering-based optical measurements of different constituents, such as glucose. By registering changes in scattering, it is possible to evalua...

journal_title：Journal of biomedical optics

authors： Kinnunen M,Myllylä R,Vainio S

更新日期：2008-03-01 00:00:00

abstract：:Heart disease is the primary cause of death in the western world. Many of these deaths are caused by the rupture of vulnerable plaque. Vulnerable plaques are characterized by a large lipid core covered by a thin fibrous cap. One method for detecting these plaques is reflection spectroscopy. Several studies have invest...

journal_title：Journal of biomedical optics

authors： Lilledahl MB,Haugen OA,Barkost M,Svaasand LO

更新日期：2006-03-01 00:00:00

abstract：:The effect of silica/gold nanoshells and titanium dioxide nanoparticles on the optical properties of skin is studied. By implementing in vivo measurements and Monte Carlo simulations, we analyze the efficiency of using these nanoparticles as contrasting agents for optical coherence tomography (OCT) imaging of skin. In...

journal_title：Journal of biomedical optics

authors： Kirillin M,Shirmanova M,Sirotkina M,Bugrova M,Khlebtsov B,Zagaynova E

更新日期：2009-03-01 00:00:00

abstract：:We propose a rate process model for describing photochemical damage to retinal cells by short wavelength laser exposures. The rate equation for photochemical damage contains a positive rate that is temperature independent, and a negative (quenching) rate that is temperature dependent. Using the traditional Arrhenius i...

journal_title：Journal of biomedical optics

authors： Clark CD 3rd,Denton ML,Thomas RJ

更新日期：2011-02-01 00:00:00

abstract：:A transgenic mouse model with a Clim [co-factor of LIM (a combination of first letters of Lin-11 (C. elegans), ISL1 (rat), and Mec-3 (C. elegans) gene names) domain proteins] gene partially blocked in the epithelial compartment of its tissues is used to establish the sensitivity of intrinsic reflectance nonlinear opti...

journal_title：Journal of biomedical optics

authors： Lyubovitsky JG,Spencer JA,Krasieva TB,Andersen B,Tromberg BJ

更新日期：2006-01-01 00:00:00

abstract：:A classifier capable of ranking structural alterations of the cytoskeleton is developed. Images of cytoskeletal microtubules obtained from the epifluorescence microscopy of primary culture rat hepatocytes are analyzed. Morphological descriptors are extracted by contour and mass fractal analysis, direct methods, and sp...

journal_title：Journal of biomedical optics

authors： Crosta GF,Urani C,Fumarola L

更新日期：2006-03-01 00:00:00

abstract：:Investigating the elasticity of ocular tissue (cornea and intraocular lens) could help the understanding and management of pathologies related to biomechanical deficiency. In previous studies, we introduced a setup based on optical coherence tomography for shear wave elastography (SWE) with high resolution and high se...

journal_title：Journal of biomedical optics

authors： Nguyen TM,Arnal B,Song S,Huang Z,Wang RK,O'Donnell M

更新日期：2015-01-01 00:00:00

abstract：:Using the multiresolution ability of wavelets and effectiveness of singular value decomposition (SVD) to identify statistically robust parameters, we find a number of local and global features, capturing spectral correlations in the co- and cross-polarized channels, at different scales (of human breast tissues). The c...

journal_title：Journal of biomedical optics

authors： Gharekhan AH,Arora S,Oza AN,Sureshkumar MB,Pradhan A,Panigrahi PK

更新日期：2011-08-01 00:00:00

abstract：:A model of infrared neural stimulation (INS) has been developed to allow the temporal characteristics of different stimulation parameters and geometries to be better understood. The model uses a finite element approach to solve the heat equation and allow detailed analysis of heat during INS with both microsecond and ...

journal_title：Journal of biomedical optics

authors： Thompson AC,Wade SA,Cadusch PJ,Brown WG,Stoddart PR

更新日期：2013-03-01 00:00:00

abstract：:Stenosis and occlusion of the superficial femoral artery (SFA) are most common in arterial occlusive disease. There are numerous interventional, surgical, and combined approaches to reconstitute maximum blood supply to the lower limb; however, despite intense clinical research, the long-term success rates are still po...

journal_title：Journal of biomedical optics

authors： Heneweer C,Siggelkow M,Helle M,Petzina R,Wulff A,Schaefer JP,Berndt R,Rusch R,Wedel T,Klaws G,Müller-Gerbl M,Röcken C,Jansen O,Lutter G,Cremer J,Groß J

更新日期：2015-02-01 00:00:00

abstract：:Confocal micro-Raman spectroscopy--a valuable analytical tool in biological and medical field of research-allows probing molecular vibrations of samples without external labels or extensive preparation. We employ confocal micro-Raman spectroscopy to characterize renal tumors and normal tissue. Results show that Raman ...

journal_title：Journal of biomedical optics

authors： Zhuang Z,Li N,Guo Z,Zhu M,Xiong K,Chen S

更新日期：2013-03-01 00:00:00

abstract：:Myocardial infarction leads to structural remodeling of the myocardium, in particular to the loss of cardiomyocytes due to necrosis and an increase in collagen with scar formation. Stem cell regenerative treatments have been shown to alter this remodeling process, resulting in improved cardiac function. As healthy myo...

journal_title：Journal of biomedical optics

authors： Wood MF,Ghosh N,Wallenburg MA,Li SH,Weisel RD,Wilson BC,Li RK,Vitkin IA

更新日期：2010-07-01 00:00:00

abstract：:Intravascular near-infrared fluorescence (NIRF) imaging offers a new approach for characterizing atherosclerotic plaque, but random catheter positioning within the vessel lumen results in variable light attenuation and can yield inaccurate measurements. We hypothesized that NIRF measurements could be corrected for var...

journal_title：Journal of biomedical optics

authors： Dixon AJ,Hossack JA

更新日期：2013-05-01 00:00:00

abstract：:Optical imaging using voltage-sensitive dyes has become an important tool for studying vortex-like electrical waves in the heart. Such waves, known as spiral or scroll waves, can spontaneously form in pathological ventricular myocardium, causing ventricular fibrillation and sudden death. Until recently, observations o...

journal_title：Journal of biomedical optics

authors： Bernus O,Mukund KS,Pertsov AM

更新日期：2007-01-01 00:00:00

abstract：:In the last decade, coherent anti-Stokes Raman scattering (CARS) microscopy has emerged as a powerful multiphoton imaging technique offering label-free chemical sensitivity and high three-dimensional resolution. However, its widespread application in the life sciences has been hampered by the use of costly pulsed lase...

journal_title：Journal of biomedical optics

authors： Li B,Borri P,Langbein W

更新日期：2013-06-01 00:00:00

abstract：:The development of accurate and clinically applicable tools to assess cancer risk is essential to define candidates to undergo screening for early-stage cancers at a curable stage or provide a novel method to monitor chemoprevention treatments. With the use of our recently developed optical technology--spatial-domain ...

journal_title：Journal of biomedical optics

authors： Bista RK,Uttam S,Hartman DJ,Qiu W,Yu J,Zhang L,Brand RE,Liu Y

更新日期：2012-06-01 00:00:00

abstract：:By applying linear perturbation theory to the radiation transport equation, the inverse problem of optical diffusion tomography can be reduced to a set of linear equations, Wμ=R, where W is the weight function, μ are the cross-section perturbations to be imaged, and R is the detector readings perturbations. We have st...

journal_title：Journal of biomedical optics

authors： Chang J,Graber HL,Barbour RL

更新日期：1998-04-01 00:00:00

abstract：:We instrumented a combined fluorescence spectroscopy and imaging system to characterize the single- and two-photon excited autofluorescence in epithelial tissue. Single-photon fluorescence (SPF) are compared with two-photon fluorescence (TPF) measured at the same location in epithelial tissue. It was found that the SP...

journal_title：Journal of biomedical optics

authors： Zheng W,Wu Y,Li D,Qu JY

更新日期：2008-09-01 00:00:00

abstract：:Selective laser targeting of the retinal pigment epithelium (RPE) is an attractive method for treating RPE-associated disorders. We are developing a method for optically detecting intracellular microcavitation that can potentially serve as an immediate feedback of the treatment outcome. Thermal denaturation or intrace...

journal_title：Journal of biomedical optics

authors： Lee H,Alt C,Pitsillides CM,Lin CP

更新日期：2007-11-01 00:00:00

abstract：:Intrauterine photoacoustic and ultrasound imaging are probe-based imaging modalities with translational potential for use in detecting endometrial diseases. This deep-tissue imaging probe design allows for the retrofitting of commercially available endometrial sampling curettes. The imaging probe presented here has a ...

journal_title：Journal of biomedical optics

authors： Miranda C,Barkley J,Smith B

更新日期：2018-04-01 00:00:00

abstract：:A quantification method to measure endocytosis was designed to assess cellular uptake and specificity of a targeting nanoparticle platform. A simple N -hydroxysuccinimide ester conjugation technique to functionalize 100-nm hollow silica nanoshell particles with fluorescent reporter fluorescein isothiocyanate and folat...

journal_title：Journal of biomedical optics

authors： Sandoval S,Mendez N,Alfaro JG,Yang J,Aschemeyer S,Liberman A,Trogler WC,Kummel AC

更新日期：2015-08-01 00:00:00

abstract：:SpineLab is a software tool developed for reconstructing neuronal feature skeletons from three-dimensional single- or multi-photon image stacks. These images often suffer from limited resolution and a low signal-to-noise ratio, making the extraction of morphometric information difficult. To overcome this limitation, w...

journal_title：Journal of biomedical optics

authors： Jungblut D,Vlachos A,Schuldt G,Zahn N,Deller T,Wittum G

更新日期：2012-07-01 00:00:00

abstract：:Recent technological advancements in the photonics industry have led to a resurgence of interest in optical glucose sensing and to realistic progress toward the development of an optical glucose sensor. Such a sensor has the potential to significantly improve the quality of life for the estimated 16 million diabetics ...

journal_title：Journal of biomedical optics

authors： McNichols RJ,Coté GL

更新日期：2000-01-01 00:00:00