Abstract:
:Waveguide evanescent field fluorescence (WEFF) microscopy is a new development that allows the imaging of contact regions between biological cells and their substratum, as well as imaging of ultrathin films such as monomolecular Langmuir-Blodgett (LB) films. Mixed-lipid monolayer systems mimicking lung surfactant were fabricated on waveguides using the LB technique and imaged by both WEFF and standard wide field epi-fluorescence microscopy. These two technologies were compared with respect to contrast, photobleaching, and sensitivity. It was found that WEFF microscopy produced images with a much higher contrast, lower photobleaching, and higher sensitivity. In addition, fine structures in the lipidic domains were observed for the first time.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Hassanzadeh A,Mittler Sdoi
10.1117/1.3569095subject
Has Abstractpub_date
2011-04-01 00:00:00pages
046022issue
4eissn
1083-3668issn
1560-2281journal_volume
16pub_type
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