Abstract:
:The expensive fabrication of current opto-microfluidic sensors is a barrier to the successful adoption of these devices in point-of-care testing. This work reports a simple inexpensive opto-microfluidic device incorporating a poly(dimethylsiloxane)-glass hybrid microfluidic chip modified with gold nanoparticles and a high-detectivity, high-stability organic photodetector. The enhancing effect of the gold nanoparticles on horseradish peroxidase-luminol-H2O2 chemiluminescence was exploited in rapid single-analyte immunoassays. The limit of detection for 17-β estradiol was 2.5 pg/ml, which is ∼200 times more sensitive than previously reported chemiluminescent immunosensors employing other organic photodetectors. Detection was also demonstrated in complex media, including natural water and blood serum.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Pires NM,Dong Tdoi
10.1117/1.JBO.19.3.030504subject
Has Abstractpub_date
2014-03-01 00:00:00pages
30504issue
3eissn
1083-3668issn
1560-2281pii
1846616journal_volume
19pub_type
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