Abstract:
:Reactive oxygen species (ROS) produced by photodynamic therapy (PDT) is recorded in vivo using a chemiluminescence (CL)-based gated optical system. A novel approach is developed to utilize the fluorescence (FL) of the CL probe as an internal fluorescence to calibrate the observed CL on pharmacokinetics of the probe in situ. The results show that during an in vivo PDT session, the intensity of CL decreases significantly and the decaying of CL is governed by fast and slow time components. By comparing the temporal profile of FL to that of the corresponding CL, it is found that the slow component is mainly attributed to the probe pharmacokinetics, whereas the fast component is likely due to rapid oxygen consumption as a result of PDT treatment. With carefully selected criteria, it is possible to minimize the effect of probe pharmacokinetics. This significantly improves the monitoring method for practical applications.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Wei Y,Xing D,Luo S,Yang L,Chen Qdoi
10.1117/1.3368688subject
Has Abstractpub_date
2010-03-01 00:00:00pages
027006issue
2eissn
1083-3668issn
1560-2281journal_volume
15pub_type
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