Abstract:
:Optical spectroscopy has shown potential as a real-time, in vivo, diagnostic tool for identifying neoplasia during endoscopy. We present the development of a diagnostic algorithm to classify elastic-scattering spectroscopy (ESS) spectra as either neoplastic or non-neoplastic. The algorithm is based on pattern recognition methods, including ensemble classifiers, in which members of the ensemble are trained on different regions of the ESS spectrum, and misclassification-rejection, where the algorithm identifies and refrains from classifying samples that are at higher risk of being misclassified. These "rejected" samples can be reexamined by simply repositioning the probe to obtain additional optical readings or ultimately by sending the polyp for histopathological assessment, as per standard practice. Prospective validation using separate training and testing sets result in a baseline performance of sensitivity = .83, specificity = .79, using the standard framework of feature extraction (principal component analysis) followed by classification (with linear support vector machines). With the developed algorithm, performance improves to Se ∼ 0.90, Sp ∼ 0.90, at a cost of rejecting 20-33% of the samples. These results are on par with a panel of expert pathologists. For colonoscopic prevention of colorectal cancer, our system could reduce biopsy risk and cost, obviate retrieval of non-neoplastic polyps, decrease procedure time, and improve assessment of cancer risk.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Rodriguez-Diaz E,Castanon DA,Singh SK,Bigio IJdoi
10.1117/1.3592488subject
Has Abstractpub_date
2011-06-01 00:00:00pages
067009issue
6eissn
1083-3668issn
1560-2281journal_volume
16pub_type
杂志文章abstract::The formation of kidney stones is a common and highly studied disease, which causes intense pain and presents a high recidivism. In order to find the causes of this problem, the characterization of the main compounds is of great importance. In this sense, the analysis of the composition and structure of the stone can ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.7.076027
更新日期:2012-07-01 00:00:00
abstract::Water content is an important factor for skin condition. The determination of the hydration state of the skin is necessary to obtain basic knowledge about the penetration and loss of water in the skin stratum corneum. Attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy is used to measure hy...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.429890
更新日期:1998-07-01 00:00:00
abstract::Computer simulation is used to facilitate the design of fiber-probe geometries that enable enhanced detection of optical signals arising from specific tissue depths. Obtaining understanding of the relationship between fiber-probe design and tissue interrogation is critical when developing strategies for optical detect...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1989335
更新日期:2005-07-01 00:00:00
abstract::We explore the use of Monte-Carlo-model-based approaches for the analysis of fluorescence and diffuse reflectance spectra measured ex vivo from breast tissues. These models are used to extract the absorption, scattering, and fluorescence properties of malignant and nonmalignant tissues and to diagnose breast cancer ba...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2931078
更新日期:2008-05-01 00:00:00
abstract::We describe the protocol for an inexpensive and nondestructive optical reflectance assay for the measurement of biofilm formation. Reflectance data are obtained using an Ocean Optics (Dunedin, Florida) USB 2000 spectrometer with a polychromatic light source. A fiber optic cable is used both for illumination and collec...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1953347
更新日期:2005-07-01 00:00:00
abstract::Raman spectroscopy is an optical technique able to interrogate biological tissues, giving us an understanding of the changes in molecular structure that are associated with disease development. The Kerr-gated Raman spectroscopy technique uses a picosecond pulsed laser as well as fast temporal gating of collected Raman...
journal_title:Journal of biomedical optics
pub_type: 临床试验,杂志文章
doi:10.1117/1.1991848
更新日期:2005-07-01 00:00:00
abstract::Mueller polarimetry is applied to study the samples of nails: natural (or reference) and irradiated to 2 Gy ionizing radiation dose. We measure the whole Mueller matrices of the samples as a function of the scattering angle at a wavelength of 632.8 nm. We apply depolarization analysis to measured Mueller matrices by c...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.7.071108
更新日期:2016-07-01 00:00:00
abstract::We present a method for tissue fluorescence quantification in situ using a handheld fiber optic probe that measures both the fluorescence and diffuse reflectance spectra. A simplified method to decouple the fluorescence spectrum from distorting effects of the tissue optical absorption and scattering is developed, with...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3523616
更新日期:2010-11-01 00:00:00
abstract::The composition of cartilage is predictive of its in vivo performance. Therefore, the ability to assess its primary macromolecular components, proteoglycan (PG) and collagen, is of great importance. In the current study, we hypothesized that PG content and distribution in tissue engineered cartilage could be determine...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1922329
更新日期:2005-05-01 00:00:00
abstract::Pulse oximeters are widely used for noninvasive monitoring of oxygen saturation in arterial blood hemoglobin. We present a transmittance pulse oximetry system based on near-infrared (NIR) laser diodes (750 and 850 nm) for monitoring oxygen saturation of arterial blood hemoglobin. The pulse oximetry system is made up o...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1578495
更新日期:2003-07-01 00:00:00
abstract:SIGNIFICANCE:A noninvasive method based on surface-enhanced Raman spectroscopy (SERS) of tears was proposed as a support for diagnosing neurodegenerative pathologies, including different forms of dementia and Alzheimer's disease (AD). In this field, timely and reliable discrimination and diagnosis are critical aspects ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.25.8.087002
更新日期:2020-08-01 00:00:00
abstract::Keratoconus is an eye disease in which the cornea progressively deforms due to loss of cornea mechanical rigidity, and thus causes deterioration of visual acuity. Techniques to characterize the mechanical characteristics of the cornea are important to better monitor changes and response to treatments. To investigate t...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.9.095005
更新日期:2017-09-01 00:00:00
abstract::Optical microanglography (OMAG) is a recently developed imaging modality capable of volumetric imaging of dynamic blood perfusion, down to capillary level resolution, with an imaging depth up to 2.00 mm beneath the tissue surface. We report the use of OMAG to monitor the cerebral blood flow (CBF) over the cortex of mo...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3207121
更新日期:2009-07-01 00:00:00
abstract::An autofluorescence lifetime wide-field imaging system that can generate contrast in underlying tissue structures of normal and malignant brain tissue samples with video rate acquisition and processing time is presented. Images of the investigated tissues were acquired with high resolution (∼35 μm) using an algorithm ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.6.060504
更新日期:2013-06-01 00:00:00
abstract::Surfactant stabilized microbubbles are widely used clinical contrast agents for ultrasound imaging. In this work, the light propagation through a turbid medium in the presence of microbubbles has been investigated. Through a series of experiments, it has been found that the optical attenuation is increased when the mi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.1.015002
更新日期:2013-01-01 00:00:00
abstract::Spatially resolved spectroscopy (SRS) allows the estimation of absolute tissue oxygen saturation, the ratio of oxygenated to total hemoglobin concentration, which may facilitate the comparison of results among patients. Eighty-two premature infants were included over two years. The cerebral tissue oxygenation index (c...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3013454
更新日期:2008-11-01 00:00:00
abstract::Limited cutaneous systemic sclerosis (lcSSc) was formerly known as CREST syndrome in reference to the associated clinical features: calcinosis, Raynaud's phenomenon, esophageal dysfunction, sclerodactyly, and telangiectasias. The transforming growth factor beta has been identified as a major player in the pathogenic p...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.11.118001
更新日期:2014-01-01 00:00:00
abstract::Three-dimensional (3-D) tissue imaging offers substantial benefits to a wide range of biomedical investigations from cardiovascular biology, diabetes, Alzheimer's disease to cancer. Two-photon tissue cytometry is a novel technique based on high-speed multiphoton microscopy coupled with automated histological sectionin...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2435626
更新日期:2007-01-01 00:00:00
abstract::A fully integrated trimodality fluorescence, diffuse optical, and x-ray computed tomography (FT/DOT/XCT) system for small animal imaging is reported in this work. The main purpose of this system is to obtain quantitatively accurate fluorescence concentration images using a multimodality approach. XCT offers anatomical...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3467495
更新日期:2010-07-01 00:00:00
abstract::A hyperspectral image projector (HIP) based on liquid crystal on silicon spatial light modulators is explained and demonstrated to generate data cubes. The HIP-constructed data cubes are three-dimensional images of the spatial distribution of spectrally resolved abundances of intracellular light-absorbing oxyhemoglobi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,收录出版
doi:10.1117/1.JBO.20.11.111215
更新日期:2015-01-01 00:00:00
abstract::A synchrotron light source dedicated to medical applications has been designed at National Institute of Radiological Sciences. The storage ring, with circumference of 80 m, is designed for acceleration of 2.3 GeV and a stored current of 420 mA. It is equipped with two multipole wigglers to produce sufficient photon fl...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1383062
更新日期:2001-07-01 00:00:00
abstract::In a previous study, we investigated physical methods to reduce whole-body, diet-related autofluorescence interference in several mouse strains through changes in animal diet. Measurements of mice with an in vivo multispectral imaging system over a 21-day period allowed for the quantification of concentration changes ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2799188
更新日期:2007-09-01 00:00:00
abstract::We describe the use of a Teflon-AF liquid core optical fiber (LCOF) geometry to enhance the collection of Raman scattering from the biochemical creatinine, dissolved in water and in urine. At short integration times, where shot noise is most troublesome, the enhanced signal leads to greater accuracy in estimating the ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1917842
更新日期:2005-05-01 00:00:00
abstract::Inexpensive near-infrared microscopy (NIRM) was developed as a convenient technique to detect the medulla loss of scalp hair while reducing analytical time with easy sample preparation, leading to a field screening tool for breast cancer. NIRM has been evaluated as an alternative to synchrotron-based nanoscopy and to ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.24.9.096501
更新日期:2019-09-01 00:00:00
abstract::Given the several difficulties associated with histology, including difficulty in continuous monitoring, this study aimed to investigate the feasibility of optical imaging modalities—cross-polarization color (CPC) imaging, erythema index (EI) imaging, and laser speckle contrast (LSC) imaging—for continuous evaluation ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.10.106010
更新日期:2014-01-01 00:00:00
abstract::We assess the properties of contact pressure applied by manually operated fiber-optic probes as a function of the operator, probe contact area, and sample stiffness. First, the mechanical properties of human skin sites with different skin structures, thicknesses, and underlying tissues were studied by in vivo indentat...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.12.127002
更新日期:2015-01-01 00:00:00
abstract::Nonviral, site-specific gene delivery to deep tissue is required for gene therapy of a spinal cord injury. However, an efficient method satisfying these requirements has not been established. This study demonstrates efficient and targeted gene transfer into the spinal cord by using photomechanical waves (PMWs), which ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3642014
更新日期:2011-10-01 00:00:00
abstract::Fluorescence lifetime imaging microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. Recent developments in multiphoton FLIM methods are reviewed and a novel multiphoton FLIM system using a streak...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1577574
更新日期:2003-07-01 00:00:00
abstract::Hemoglobin (Hb), as one of main components of blood, has a unique quaternary structure. Its release of oxygen is controlled by oxygen partial pressure (PO2). We investigate the specific spectroscopic changes in Hb under different PO2 levels to optimize clinical methods of measuring tissue PO2. The transmissivity of Hb...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.12.125002
更新日期:2012-12-01 00:00:00
abstract::We have developed a novel nitroimidazole indocyanine dye conjugate for tumor-targeted hypoxia fluorescence tomography. The hypoxia probe has been evaluated in vitro using tumor cell lines and in vivo with tumor targeting in mice. The in vitro cell studies were performed to assess fluorescence labeling differences betw...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3589348
更新日期:2011-06-01 00:00:00