Abstract:
:Fluorescence lifetime imaging microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. Recent developments in multiphoton FLIM methods are reviewed and a novel multiphoton FLIM system using a streak camera is described. An example of a typical application of the system is provided in which the fluorescence resonance energy transfer between a donor-acceptor pair of fluorescent proteins within a cellular specimen is measured.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Krishnan RV,Masuda A,Centonze VE,Herman Bdoi
10.1117/1.1577574subject
Has Abstractpub_date
2003-07-01 00:00:00pages
362-7issue
3eissn
1083-3668issn
1560-2281journal_volume
8pub_type
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