Abstract:
:The development of methods that allow microscale studies of complex biomaterials based on their molecular composition is of great interest to a wide range of research fields. We show that stimulated Raman scattering (SRS) microscopy is an excellent analytical tool to study distributions of different biomolecules in multiphasic systems. SRS combines the label-free molecular specificity of vibrational spectroscopy with an enhanced sensitivity due to coherent excitation of molecular vibrations. Compared to previous imaging studies using coherent anti-Stokes Raman scattering microscopy, the main advantage of SRS microscopy is the absence of the unwanted nonresonant background, which translates into a superior sensitivity and undistorted vibrational spectra. We compare spectra of complex materials obtained with stimulated Raman scattering and spontaneous Raman scattering in the crowded fingerprint region. We find that, as expected, there is excellent correspondence and that the SRS spectra are free from interference from background fluorescence. In addition, we show high-resolution imaging of the distributions of selected biomolecules, such as lipids and proteins, in food products with SRS microscopy.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Roeffaers MB,Zhang X,Freudiger CW,Saar BG,van Ruijven M,van Dalen G,Xiao C,Xie XSdoi
10.1117/1.3516591subject
Has Abstractpub_date
2010-11-01 00:00:00pages
066016issue
6eissn
1083-3668issn
1560-2281journal_volume
15pub_type
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