Spectral imaging technique for retinal perfusion detection using confocal scanning laser ophthalmoscopy.

abstract：:Diffuse near-infrared tomography of tissue reveals scattering changes that originate from the submicroscopic features of the tissue; yet the existing tools to use this information to predict which features contribute to the scattering spectrum are limited by the lack of direct data quantifying the particle sizes. Brea...

journal_title：Journal of biomedical optics

authors： Bartek M,Wang X,Wells W,Paulsen KD,Pogue BW

更新日期：2006-11-01 00:00:00

abstract：:We used two-photon laser scanning microscopy (TPLSM) to demonstrate for the first time its potential in studying relational details at the cellular level of atherogenesis in intact, viable mouse carotid arteries. Isolated and mounted arteries of ApoE-/-mice, aged 15 or 21 weeks (7 and 13 weeks on western diet), were i...

journal_title：Journal of biomedical optics

authors： Megens RT,oude Egbrink MG,Merkx M,Slaaf DW,van Zandvoort MA

更新日期：2008-07-01 00:00:00

abstract：:Cancer is the main cause of canine morbidity and mortality. The existing evaluation of tumors requires an experienced veterinarian and usually includes invasive procedures (e.g., fine-needle aspiration) that can be unpleasant for the dog and the owner. We investigate visible and near-infrared diffuse reflectance spect...

journal_title：Journal of biomedical optics

authors： Cugmas B,Plavec T,Bregar M,Naglič P,Pernuš F,Likar B,Bürmen M

更新日期：2015-03-01 00:00:00

abstract：:Infrared microscopic imaging has been utilized to analyze for the first time the spatial distribution of lipid structure in an ex vivo human organ culture skin wound healing model. Infrared images were collected at zero, two, four, and six days following wounding. Analysis of lipid infrared spectral properties reveale...

journal_title：Journal of biomedical optics

authors： Yu G,Stojadinovic O,Tomic-Canic M,Flach CR,Mendelsohn R

更新日期：2012-09-01 00:00:00

abstract：:We describe a computer eye model that allows for aspheric surfaces and a three-dimensional computer-based ray-tracing technique to simulate optical properties of the human eye and visual perception under various eye defects. Eye surfaces, such as the cornea, eye lens, and retina, are modeled or approximated by a set o...

journal_title：Journal of biomedical optics

authors： Fink W,Micol D

更新日期：2006-09-01 00:00:00

abstract：:Spectrally resolved two-photon excited autofluorescence imaging is used to distinguish different cell types and functional areas during dynamic processes in the living gut. Excitation and emission spectra of mucosal tissue and tissue components are correlated to spectra of endogenous chromophores. We show that selecti...

journal_title：Journal of biomedical optics

authors： Orzekowsky-Schroeder R,Klinger A,Martensen B,Blessenohl M,Gebert A,Vogel A,Hüttmann G

更新日期：2011-11-01 00:00:00

abstract：:Cortical spreading depression (CSD) is a slowly propagating wave of depolarization of neurons and glia and has a less characterized vascular component. CSD is a commonly used phenomenon to test new methods of live brain imaging. Application of a blood pulsations imaging (BPI) technique to study of CSD induced with hig...

journal_title：Journal of biomedical optics

authors： Teplov V,Shatillo A,Nippolainen E,Gröhn O,Giniatullin R,Kamshilin AA

更新日期：2014-04-01 00:00:00

abstract：:Frequency-domain diffuse optical spectroscopy (FD-DOS) utilizes intensity-modulated light to characterize optical scattering and absorption in thick tissue. Previous FD-DOS systems have been limited by large device footprints, complex electronics, high costs, and limited acquisition speeds, all of which complicate acc...

journal_title：Journal of biomedical optics

authors： Torjesen A,Istfan R,Roblyer D

更新日期：2017-03-01 00:00:00

abstract：:We report a photonic approach for selective inactivation of viruses with a near-infrared subpicosecond laser. We demonstrate that this method can selectively inactivate viral particles ranging from nonpathogenic viruses such as the M13 bacteriophage and the tobacco mosaic virus to pathogenic viruses such as the human ...

journal_title：Journal of biomedical optics

authors： Tsen KT,Tsen SW,Fu Q,Lindsay SM,Kibler K,Jacobs B,Wu TC,Karanam B,Jagu S,Roden RB,Hung CF,Sankey OF,Ramakrishna B,Kiang JG

更新日期：2009-11-01 00:00:00

abstract：:The objective of the study is the quantitative analysis of the dose-time dependences of changes occurring in collagen of bladder and rectum after gamma-irradiation using optical methods [nonlinear microscopy in a second harmonic generation (SHG) detection regime and cross-polarization optical coherence tomography (CP ...

journal_title：Journal of biomedical optics

authors： Kochueva M,Dudenkova V,Kuznetsov S,Varlamova A,Sergeeva E,Kiseleva E,Maslennikova A

更新日期：2018-08-01 00:00:00

abstract：:The metabolic rate of oxygen consumption, an important indicator of tissue metabolism, can be expressed as the change of net blood oxygen flux into and out of a tissue region per 100 g of tissue. In this work, we propose a photoacoustic and Doppler ultrasound method for imaging local blood oxygen flux of a single vess...

journal_title：Journal of biomedical optics

authors： Jiang Y,Forbrich A,Harrison T,Zemp RJ

更新日期：2012-03-01 00:00:00

abstract：:Pulse-modulated second harmonic imaging microscopes (PM-SHIMs) exhibit improved signal-to-noise ratio (SNR) over conventional SHIMs on sensitive imaging and quantification of weak collagen signals inside tissues. We quantify the spatial distribution of sparse collagen inside a xenograft model of human acute myeloid le...

journal_title：Journal of biomedical optics

authors： Raja AM,Xu S,Sun W,Zhou J,Tai DC,Chen CS,Rajapakse JC,So PT,Yu H

更新日期：2010-09-01 00:00:00

abstract：:Dynamic analyses of vibrational motion in cell membranes provide a lot of information on the complex dynamic motilities of a red blood cell (RBC). Here, we present the correlation properties of membrane fluctuation in discocyte and spherocyte RBCs by using quantitative phase microscopy (QPM). Since QPM can provide nan...

journal_title：Journal of biomedical optics

authors： Lee S,Lee JY,Park CS,Kim DY

更新日期：2011-07-01 00:00:00

abstract：:Intracellular oxygen concentration is of primary importance in determining numerous physiological and pathological processes in biological systems. In this paper, we describe the application of the oxygen sensing indicator, ruthenium dibipyridine 4-(1"-pyrenyl)-2,2'-bipyridine chloride [Ru(bpy-pyr)(bpy)(2)], for molec...

journal_title：Journal of biomedical optics

authors： Ji J,Rosenzweig N,Jones I,Rosenzweig Z

更新日期：2002-07-01 00:00:00

abstract：:Fourier domain (FD) techniques have increasingly gained attention in optical coherence tomography (OCT). This is primarily due to their demonstrated sensitivity of two to three orders of magnitude over conventional time-domain techniques. FDOCT images are subject to two primary sources of artifacts. First, a complex c...

journal_title：Journal of biomedical optics

authors： Davis AM,Choma MA,Izatt JA

更新日期：2005-11-01 00:00:00

abstract：:A new skin-imaging method, which was developed recently, gives an informative image data about skin moles by collecting scattered polarized light reflected from their tissue area at different wavelengths and polarizations. This method is called as optical rotational spectropolarimetric imaging (ORSI), which scans the ...

journal_title：Journal of biomedical optics

authors： Graham L,Yitzhaky Y,Abdulhalim I

更新日期：2013-11-01 00:00:00

abstract：:Sugar-rich diets and poor dental hygiene promote the formation of a biofilm (plaque) that strongly adheres to the dental enamel surface and fosters the evolution of aciduric bacteria. The acid contributes to demineralization of the exterior tooth enamel, which accelerates after the pH drops below a critical value (∼5....

journal_title：Journal of biomedical optics

authors： Sharma M,Graham JY,Walczak PA,Nguyen R,Lee LK,Carson MD,Nelson LY,Patel SN,Xu Z,Seibel EJ

更新日期：2019-01-01 00:00:00

abstract：:The effect of glucose on fluorescence of synthesized ZnCdS nanoparticles in the presence of glucose oxidase or in a mixture of glucose oxidase and peroxidase has been investigated. Behavior of fluorescence characteristics of ZnCdS nanoparticles with nonstabilized surface and coated with polymer shell is compared. It h...

journal_title：Journal of biomedical optics

authors： Kochubey VI,Volkova EK,Konyukhova JG

更新日期：2014-01-01 00:00:00

abstract：:We have used an adaptive optics confocal scanning laser ophthalmoscope to assess oxygen saturation in small retinal vessels. Images of the vessels with a diameter smaller than 50 μm are recorded at oxygen sensitive and isosbestic wavelengths (680 and 796 nm, respectively). The vessel optical densities (ODs) are determ...

journal_title：Journal of biomedical optics

authors： Li H,Lu J,Shi G,Zhang Y

更新日期：2011-11-01 00:00:00

abstract：:Early detection of tissue hypoxia in the intensive care unit is essential for effective treatment. Reduced nicotinamide adenine dinucleotide (NADH) has been suggested to be the most sensitive indicator of tissue oxygenation at the mitochondrial level. However, no experimental evidence comparing the kinetics of changes...

journal_title：Journal of biomedical optics

authors： Shi H,Sun N,Mayevsky A,Zhang Z,Luo Q

更新日期：2014-01-01 00:00:00

abstract：:We have combined a confocal laser scanning head modified for TPE (two-photon excitation) microscopy with some spectroscopic modules to study single molecules and molecular aggregates. The behavior of the TPE microscope unit has been characterized by means of point spread function measurements and of the demonstration ...

journal_title：Journal of biomedical optics

authors： Diaspro A,Chirico G,Federici F,Cannone F,Beretta S,Robello M

更新日期：2001-07-01 00:00:00

abstract：:Alcohol testing is an expanding area of interest due to the impacts of alcohol abuse that extend well beyond drunk driving. However, existing approaches such as blood and urine assays are hampered in some testing environments by biohazard risks. A noninvasive, in vivo spectroscopic technique offers a promising alterna...

journal_title：Journal of biomedical optics

authors： Ridder TD,Ver Steeg BJ,Laaksonen BD

更新日期：2009-09-01 00:00:00

abstract：:We present a multimodal optical microscope that incorporates six imaging modalities on one common platform. The imaging modalities include three staring modes, optical quadrature microscopy (OQM), differential interference contrast (DIC) microscopy, and epi-fluorescence microscopy, and three scanning modes, confocal r...

journal_title：Journal of biomedical optics

authors： Warger WC 2nd,Laevsky GS,Townsend DJ,Rajadhyaksha M,DiMarzio CA

更新日期：2007-07-01 00:00:00

abstract：:Reduction of optical scattering in turbid biological tissues using nonreactive chemical agents has potential applications for light-based diagnostics and therapeutics. Optical clearing effects by exogenous chemical agents, in particular sugars and sugar alcohols, have been found to be temporary with tissue rehydration...

journal_title：Journal of biomedical optics

authors： Yeh AT,Hirshburg J

更新日期：2006-01-01 00:00:00

abstract：:Muscle blood flow (MBF) and muscle oxygen saturation (SmO(2)) were measured at eight locations (four proximal, four distal) over a 4 x 8 cm(2) area of the vastus lateralis at rest and immediately after isometric, maximal quadriceps contraction using multichannel, frequency-domain, near-infrared spectroscopy. A venous ...

journal_title：Journal of biomedical optics

authors： Quaresima V,Ferrari M,Franceschini MA,Hoimes ML,Fantini S

更新日期：2004-03-01 00:00:00

abstract：:As small animal optical imaging and tomography are gaining popularity for interrogating functional and molecular events in vivo, it becomes increasingly necessary to gain knowledge of the optical properties of the species investigated to better understand and describe photon propagation through their tissues. To achie...

journal_title：Journal of biomedical optics

authors： Niedre MJ,Turner GM,Ntziachristos V

更新日期：2006-11-01 00:00:00

abstract：:Image correlation spectroscopy (ICS) is a powerful technique for detecting arrangement of fluorophores in images. This tutorial gives background into the mathematical underpinnings of ICS, specifically image autocorrelation. The effects of various artifacts and image processing steps, including background subtraction,...

journal_title：Journal of biomedical optics

authors： Robertson C,George SC

更新日期：2012-08-01 00:00:00

abstract：:A synchrotron light source dedicated to medical applications has been designed at National Institute of Radiological Sciences. The storage ring, with circumference of 80 m, is designed for acceleration of 2.3 GeV and a stored current of 420 mA. It is equipped with two multipole wigglers to produce sufficient photon fl...

journal_title：Journal of biomedical optics

authors： Torikoshi M,Tsunoo T,Endo M,Noda K,Kumada M,Yamada S,Soga F,Hyodo K

更新日期：2001-07-01 00:00:00

abstract：:Optical technologies may be applied to multiple facets of spinal surgery from diagnostics to intraoperative image guidance to therapeutics. In diagnostics, the current standard remains cross-sectional static imaging. Optical surface scanning tools may have an important role; however, significant work is required to cl...

journal_title：Journal of biomedical optics

authors： Guha D,Yang VXD

更新日期：2018-06-01 00:00:00

abstract：PURPOSE:Investigations of foveal aberrations assume circular pupils. However, the pupil becomes increasingly elliptical with increase in visual field eccentricity. We address this and other issues concerning peripheral aberration specification. METHODS:One approach uses an elliptical pupil similar to the actual pupil ...

journal_title：Journal of biomedical optics

authors： Charman WN,Mathur A,Scott DH,Hartwig A,Atchison DA

更新日期：2012-02-01 00:00:00