Abstract:
:We propose a handheld projective imaging device for orthotopic projection of near-infrared fluorescence images onto target biological tissue at visible wavelengths without any additional visual aid. The device integrates a laser diode light source module, a camera module, a projector, an ultrasonic distance sensor, a Raspberry Pi single-board computer, and a battery module in a rugged handheld unit. It is calibrated at the detected working distance for seamless coregistration between fluorescence emission and projective imaging at the target tissue site. The proposed device is able to achieve a projection resolution higher than 314 μm and a planar projection bias less than 1 mm at a projection field of view of 58 × 108 mm2 and a working distance of 27 cm. Technical feasibility for projective imaging is verified in an ex vivo model of chicken breast tissue using indocyanine green as a fluorescence agent. Clinical utility for image-guided surgery is demonstrated in a clinical trial where sentinel lymph nodes in breast cancer patients are identified and resected under the guidance of projective imaging. Our ex vivo and in vivo experiments imply the clinical utility of deploying the proposed device for image-guided surgical interventions in resource-limited settings.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Li C,Liu P,Shao P,Pei J,Li Y,Pawlik TM,Martin EW,Xu RXdoi
10.1117/1.JBO.24.8.080503subject
Has Abstractpub_date
2019-08-01 00:00:00pages
1-4issue
8eissn
1083-3668issn
1560-2281pii
JBO-190156LRjournal_volume
24pub_type
杂志文章abstract::Our purpose is to evaluate intramucosal gastric pH video imaging by 2('),7(')-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) fluorescence ratio techniques. We use a video endoscopic imaging system and BCECF as the pH fluorescent probe. Systemic in vivo pH variations are studied in 10 pigs: five in the control group ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2821698
更新日期:2007-11-01 00:00:00
abstract::Treatment of deep venous thrombosis (DVT)--a primary cause of potentially fatal pulmonary embolism (PE)--depends on the age of the thrombus. The existing clinical imaging methods are capable of visualizing a thrombus but cannot determine the age of the blood clot. Therefore, there is a need for an imaging technique to...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2992175
更新日期:2008-09-01 00:00:00
abstract::Microvascular network of the retina plays an important role in diagnosis and monitoring of various retinal diseases. We propose a three-dimensional (3-D) segmentation method with intensity-based Doppler variance (IBDV) based on swept-source optical coherence tomography. The automatic 3-D segmentation method is used to...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.7.076003
更新日期:2015-07-01 00:00:00
abstract::Vascular leakage is an important feature of various disease conditions. In vivo optical imaging provides a great opportunity for the evaluation of this phenomenon. In the present study, we focus on the development and validation of a near-infrared (NIR) imaging formula to allow reliable, cost-efficient evaluation of v...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.1.016022
更新日期:2015-01-01 00:00:00
abstract::The use of fluorescence video imaging to guide surgery is rapidly expanding, and improvements in camera readout dynamic range have not matched display capabilities. Logarithmic intensity compression is a fast, single-step mapping technique that can map the useable dynamic range of high-bit fluorescence images onto the...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.8.080504
更新日期:2015-08-01 00:00:00
abstract::High-speed (video-rate) fluorescence lifetime imaging (FLIM) through a flexible endoscope is reported based on gated optical image intensifier technology. The optimization and potential application of FLIM to tissue autofluorescence for clinical applications are discussed. ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2102807
更新日期:2005-09-01 00:00:00
abstract::This study demonstrates the use of optical spectroscopy for monitoring tumor oxygenation and metabolism in response to hyperoxic gas breathing. Hemoglobin saturation and redox ratio were quantified for a set of 14 and 9 mice, respectively, measured at baseline and during carbogen breathing (95% O(2), 5% CO(2)). In par...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3103586
更新日期:2009-03-01 00:00:00
abstract::Intraductal carcinoma is a precancerous lesion of the breast and the immediate precursor of invasive ductal carcinoma. Multiphoton microscopy (MPM) was used to monitor the progression from intraductal carcinoma to invasive ductal carcinoma, which can improve early detection of precursor lesions and halt progression to...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.9.096007
更新日期:2015-09-01 00:00:00
abstract::The measurement of changes in blood volume in tissue is important for monitoring the effects of a wide range of therapeutic interventions, from radiation therapy to skin-flap transplants. Many systems available for purchase are either expensive or difficult to use, limiting their utility in the clinical setting. A low...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.10.105005
更新日期:2014-01-01 00:00:00
abstract::We present the design and experimental results of a new MEMS-based endoscopic optical coherence tomography (OCT) probe. The uniqueness of this miniature OCT imaging probe is a two-axis MEMS mirror with through-silicon vias (TSVs) for interconnecting. The TSV interconnection enables ultracompact probe design, successfu...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3533323
更新日期:2011-02-01 00:00:00
abstract::Oblique incidence reflectometry (OIR) is an established technique for the estimation of tissue optical properties. However, a sensing footprint of a few transport mean-free paths is often needed when diffusion-regime-based algorithms are used. Smaller-footprint probes require improved light-propagation models and inve...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.6.067005
更新日期:2013-06-01 00:00:00
abstract::Image correlation spectroscopy (ICS) is a powerful technique for detecting arrangement of fluorophores in images. This tutorial gives background into the mathematical underpinnings of ICS, specifically image autocorrelation. The effects of various artifacts and image processing steps, including background subtraction,...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.8.080801
更新日期:2012-08-01 00:00:00
abstract::We have developed a novel parallel-plate diffuse optical tomography (DOT) system for three-dimensional in vivo imaging of human breast tumor based on large optical data sets. Images of oxy-, deoxy-, and total hemoglobin concentration as well as blood oxygen saturation and tissue scattering were reconstructed. Tumor ma...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3103325
更新日期:2009-03-01 00:00:00
abstract::We experimentally study potential mechanisms by which the enhancement factor in low-coherence enhanced backscattering (LEBS) can probe subtle variations in radial intensity distribution in weakly scattering media. We use enhanced backscattering of light by implementing either (1) low spatial coherence illumination or ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3443795
更新日期:2010-05-01 00:00:00
abstract::Optoacoustic (photoacoustic) diagnostic modality is a technique that combines high optical contrast and ultrasound spatial resolution. We proposed using the optoacoustic technique for a number of applications, including cancer detection, monitoring of thermotherapy (hyperthermia, coagulation, and freezing), monitoring...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.9.091512
更新日期:2017-09-01 00:00:00
abstract::There are several pathologies whose study and diagnosis is impaired by a relatively small number of documented cases. A practical approach to overcome this obstacle and advance the research in this area consists in employing computer simulations to perform controlled in silico experiments. The results of these experim...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.9.097005
更新日期:2012-09-01 00:00:00
abstract::Traditional phase-resolved Doppler optical coherence tomography (DOCT) has been reported to have potential for characterizing local fluid flow within a microporous scaffold. In this work, we apply Doppler optical microangiography (DOMAG), a new imaging technique developed by combining optical microangiography (OMAG) w...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3233590
更新日期:2009-09-01 00:00:00
abstract::Clinical imaging techniques for the anterior segment of the eye provide excellent anatomical information, but molecular imaging techniques are lacking. Molecular photoacoustic imaging is one option to address this need, but implementation requires use of contrast agents to distinguish molecular targets from background...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.24.5.056004
更新日期:2019-05-01 00:00:00
abstract::Microscopic visualization of islets of Langerhans under normal and diabetic conditions is essential for understanding the pathophysiology of the disease. The intrinsic opacity of pancreata, however, limits optical accessibility for high-resolution light microscopy of islets in situ. Because the standard microtome-base...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3470241
更新日期:2010-07-01 00:00:00
abstract::When irradiated with nanosecond laser pulses, gold nanoparticles allow for manipulation or destruction of cells and proteins with high spatial and temporal precision. Gold nanorods are especially attractive, because they have an up-to-20-fold stronger absorption than a sphere of equal volume, which is shifted to the o...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.5.058003
更新日期:2012-05-01 00:00:00
abstract::Diffuse near-infrared tomography of tissue reveals scattering changes that originate from the submicroscopic features of the tissue; yet the existing tools to use this information to predict which features contribute to the scattering spectrum are limited by the lack of direct data quantifying the particle sizes. Brea...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2398903
更新日期:2006-11-01 00:00:00
abstract::Real-time medical imaging systems such as reflectance confocal microscopes and optical coherence microscopes are being tested in multiple-patient and multiple-center clinical trials. The modulation transfer function (MTF) of these systems at any given time influences the image information content and can affect the in...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2779352
更新日期:2007-09-01 00:00:00
abstract::Skin cancer diagnosis depends not only on histopathological examination but also on visual inspection before and after the excision of suspected lesion. Neoplasm is accompanied with changes in birefringence of collagen, pleomorphicity, and hyperchromatic state of epithelial nuclei. These phenomena can be measured by s...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3609003
更新日期:2011-08-01 00:00:00
abstract::Fluorescence lifetime imaging (FLi) could potentially improve exogenous near-infrared (NIR) fluorescence imaging, because it offers the capability of discriminating a signal of interest from background, provides real-time monitoring of a chemical environment, and permits the use of several different fluorescent dyes h...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3368997
更新日期:2010-03-01 00:00:00
abstract::Utilizing a laser scanning confocal microscope system, the refractive indices of articular cartilage (AC) with mechanical or biochemical degenerations were characterized to investigate whether potential correlations exist between refractive index (RI) and cartilage degeneration. The cartilage samples collected from th...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.9.095002
更新日期:2016-09-01 00:00:00
abstract::A promising method to achieve rapid convergence for image reconstruction is introduced for the continuous-wave near-infrared (NIR) diffuse optical tomography (DOT). Tomographic techniques are usually implemented off line and are time consuming to realize image reconstruction, especially for NIR DOT. Therefore, it is e...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3299727
更新日期:2010-01-01 00:00:00
abstract::The acousto-optic deflector (AOD) is highly preferred in laser scanning microscopy for its fast scanning ability and random-addressing capability. However, its application in two-photon microscopy is frustrated by the dispersion of the AOD, which results in beam distortion and pulse lengthening. We report the analysis...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2714061
更新日期:2007-03-01 00:00:00
abstract::We instrumented a combined fluorescence spectroscopy and imaging system to characterize the single- and two-photon excited autofluorescence in epithelial tissue. Single-photon fluorescence (SPF) are compared with two-photon fluorescence (TPF) measured at the same location in epithelial tissue. It was found that the SP...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2975866
更新日期:2008-09-01 00:00:00
abstract::In the last decade, coherent anti-Stokes Raman scattering (CARS) microscopy has emerged as a powerful multiphoton imaging technique offering label-free chemical sensitivity and high three-dimensional resolution. However, its widespread application in the life sciences has been hampered by the use of costly pulsed lase...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.6.066004
更新日期:2013-06-01 00:00:00
abstract::We explore the use of Monte-Carlo-model-based approaches for the analysis of fluorescence and diffuse reflectance spectra measured ex vivo from breast tissues. These models are used to extract the absorption, scattering, and fluorescence properties of malignant and nonmalignant tissues and to diagnose breast cancer ba...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2931078
更新日期:2008-05-01 00:00:00