Abstract:
:The acousto-optic deflector (AOD) is highly preferred in laser scanning microscopy for its fast scanning ability and random-addressing capability. However, its application in two-photon microscopy is frustrated by the dispersion of the AOD, which results in beam distortion and pulse lengthening. We report the analysis of simultaneous compensation for the angular dispersion and temporal dispersion of the AOD by merely introducing a single dispersive element such as a prism or a grating. Besides serving as a scanner, the AOD is also a part of the compressor pair by integrating the dispersive nature of the AO interaction. This compensation principle is effective for both one-dimensional (1-D) AOD and two-dimensional (2-D) AOD scanning. Switching from a 1-D to a 2-D system requires proper optical alignment with the compensation element, but does not involve any new components. Analytical expressions are given to illustrate the working principle and to help with understanding the design of the system. Fluorescence images of beads and cells are shown to demonstrate the performance of two-photon microscopy when applying this compensated 2-D AOD as scanner.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Zeng S,Lv X,Bi K,Zhan C,Li D,Chen WR,Xiong W,Jacques SL,Luo Qdoi
10.1117/1.2714061subject
Has Abstractpub_date
2007-03-01 00:00:00pages
024015issue
2eissn
1083-3668issn
1560-2281journal_volume
12pub_type
杂志文章abstract::We present a novel fluorescence imaging system developed for real-time interventional imaging applications. The system implements a correction scheme that improves the accuracy of epi-illumination fluorescence images for light intensity variation in tissues. The implementation is based on the use of three cameras oper...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3259362
更新日期:2009-11-01 00:00:00
abstract::In near-infrared spectroscopy, concentration changes in oxygenated and deoxygenated hemoglobin are calculated from the changes in the attenuation of the measurement light. This is done by solving a linear equation based on the modified Lambert-Beer law. To solve this equation, we need to know the partial optical pathl...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3147402
更新日期:2009-05-01 00:00:00
abstract::Cochlear implantation offers the potential to restore sensitive hearing in patients with severe to profound deafness. However, surgical placement of the electrode array within the cochlea can produce trauma to sensorineural components, particularly if the initial turn of the cochlea is not successfully navigated as th...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.5.057004
更新日期:2014-05-01 00:00:00
abstract::Lucigenin- and luminol-dependent chemiluminescence [(LC-CL) and (LM-CL)] in nondiluted human blood was studied. LM-CL was low in fresh blood and disappeared after its storage for 3 h, though the respiratory burst (RB) stimulation in blood was followed by high intensity and long-lasting LM-CL. LC-CL was high in fresh b...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.429921
更新日期:1999-01-01 00:00:00
abstract::Stenosis and occlusion of the superficial femoral artery (SFA) are most common in arterial occlusive disease. There are numerous interventional, surgical, and combined approaches to reconstitute maximum blood supply to the lower limb; however, despite intense clinical research, the long-term success rates are still po...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.2.025005
更新日期:2015-02-01 00:00:00
abstract::Vectorial models of focused beams are important to a variety of fields including microscopy, lithography, optical physics, and biomedical imaging. This has led to many models being developed, which calculate how beams of various profiles are focused both in free space and in the presence of stratified media. The major...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.23.9.090801
更新日期:2018-09-01 00:00:00
abstract::Confocal micro-Raman spectroscopy--a valuable analytical tool in biological and medical field of research-allows probing molecular vibrations of samples without external labels or extensive preparation. We employ confocal micro-Raman spectroscopy to characterize renal tumors and normal tissue. Results show that Raman ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.3.031103
更新日期:2013-03-01 00:00:00
abstract::The morphogenetic relationship between early patterning and polarity formation is of fundamental interest and remains a controversial issue in preimplantation embryonic development. We use a label-free three-dimensional (3-D) imaging technique of full-field optical coherence tomography (FF-OCT) successfully for the fi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.1.010503
更新日期:2013-01-01 00:00:00
abstract::Raman spectroscopy on single, living epithelial cells captured in a laser trap is shown to have diagnostic power over colorectal cancer. This new single-cell technology comprises three major components: primary culture processing of human tissue samples to produce single-cell suspensions, Raman detection on singly tra...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2748060
更新日期:2007-05-01 00:00:00
abstract::Second-harmonic-generation (SHG) microscopy has emerged as a powerful tool to image unstained living tissues and probe their molecular and supramolecular organization. In this article, we review the physical basis of SHG, highlighting how coherent summation of second-harmonic response leads to the sensitivity of polar...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.JBO.17.6.060901
更新日期:2012-06-01 00:00:00
abstract::Discrete form sensitivity functions are derived for a number of measurement types employed in autocorrelation-based ultrasound-modulated optical tomography. The Jacobian for a particular problem is constructed from the set of such sensitivity functions arising from a raster scan of a focused ultrasound field through a...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.12.126020
更新日期:2013-12-01 00:00:00
abstract::A method is proposed for determining the glucose concentration on the human fingertip by extracting two optical parameters, namely the optical rotation angle and the depolarization index, using a Mueller optical coherence tomography technique and a genetic algorithm. The feasibility of the proposed method is demonstra...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.23.4.047001
更新日期:2018-04-01 00:00:00
abstract::Laser speckle contrast imaging is a technique used for imaging blood flow without scanning. Though several studies have attempted to combine spatial and temporal statistics of laser speckle images for reducing image noise as well as preserving acceptable spatiotemporal resolution, the statistical accuracy of these spa...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3290804
更新日期:2010-01-01 00:00:00
abstract::Our current research in the framework of an interdisciplinary project focuses on modelling the dynamics of the hemoglobin reoxygenation process in post-mortem human skin by reflectance spectrometry. The observations of reoxygenation of hemoglobin in livores after postmortem exposure to a cold environment relate the re...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.1.017004
更新日期:2013-01-01 00:00:00
abstract::In part I of the study, two attachment systems [O-ring; bar-clip (BC)] were used, and the system with three individualized O-rings provided the lowest stress on the implants and the support tissues. Therefore, the aim of this study was to assess the stress distribution, through the photoelastic method, on implant-reta...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.6.066012
更新日期:2014-06-01 00:00:00
abstract::Near-infrared spectroscopy is a novel imaging technique potentially sensitive to both brain hemodynamics (slow signal) and neuronal activity (fast optical signal, FOS). The big challenge of measuring FOS noninvasively lies in the presumably low signal-to-noise ratio. Thus, detectability of the FOS has been controversi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3505007
更新日期:2010-11-01 00:00:00
abstract::Imaging technologies working in the spatial frequency domain are becoming increasingly popular for generating wide-field maps of optical properties, enabling rapid analysis of tissue parameters. While acquisition methods have become faster and are now performing in real-time, processing methods remain slow, precluding...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.11.110501
更新日期:2016-11-01 00:00:00
abstract::The development of methods that allow microscale studies of complex biomaterials based on their molecular composition is of great interest to a wide range of research fields. We show that stimulated Raman scattering (SRS) microscopy is an excellent analytical tool to study distributions of different biomolecules in mu...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3516591
更新日期:2010-11-01 00:00:00
abstract::The epithelium (EP) thickness and the standard deviation (SD) of A-mode scan intensity in the laminar propria (LP) layer are used as effective indicators for the diagnosis of oral submucous fibrosis (OSF) based on the noninvasive clinical scanning of a swept-source optical coherence tomography (OCT) system of approxim...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3233653
更新日期:2009-09-01 00:00:00
abstract::Selective retina treatment (SRT) is a novel short pulsed laser therapy of several retinal diseases associated with a decreased metabolism at the retinal pigment epithelium (RPE). The range of laser pulse energies is small, in which the desired selective RPE disintegration is achieved without adverse effects to the neu...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2339815
更新日期:2006-07-01 00:00:00
abstract::Scanning laser ophthalmoscopy (SLO) and optical coherence tomography (OCT) provide complementary views of the retina, with the former collecting fluorescence data with good lateral but relatively low-axial resolution, and the latter collecting label-free backscattering data with comparable lateral but much higher axia...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.12.126005
更新日期:2015-01-01 00:00:00
abstract::A method for the prediction of the average photon pathlength in turbid media has been developed. The method is based on spatially resolved diffuse reflectance with discrete source detector distances up to 2 mm. Light reflectance was simulated using a Monte Carlo technique with a one-layer model utilizing a wide range ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1482378
更新日期:2002-07-01 00:00:00
abstract::Optical spectroscopy, imaging, and therapy tissue phantoms must have the scattering and absorption properties that are characteristic of human tissues, and over the past few decades, many useful models have been created. In this work, an overview of their composition and properties is outlined, by separating matrix, s...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.2335429
更新日期:2006-07-01 00:00:00
abstract::We demonstrate the first application of Raman spectroscopy in diagnosing nonmalignant, premalignant, malignant, and metastatic stages of breast cancer in a three-dimensional (3-D) cell culture model that closely mimics an in vivo environment. Comprehensive study comparing classification in two-dimensional (2-D) and 3-...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.11.117008
更新日期:2013-11-01 00:00:00
abstract::Abdominal surgeries carry considerable risk of gastrointestinal and intra-abdominal hemorrhage, which could possibly cause patient death. Photoacoustic imaging is one solution to overcome this challenge by providing visualization of major blood vessels during surgery. We investigate the feasibility of in vivo blood ve...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.24.12.121905
更新日期:2019-08-01 00:00:00
abstract::Myocardial infarction leads to structural remodeling of the myocardium, in particular to the loss of cardiomyocytes due to necrosis and an increase in collagen with scar formation. Stem cell regenerative treatments have been shown to alter this remodeling process, resulting in improved cardiac function. As healthy myo...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3469844
更新日期:2010-07-01 00:00:00
abstract::When irradiated with nanosecond laser pulses, gold nanoparticles allow for manipulation or destruction of cells and proteins with high spatial and temporal precision. Gold nanorods are especially attractive, because they have an up-to-20-fold stronger absorption than a sphere of equal volume, which is shifted to the o...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.5.058003
更新日期:2012-05-01 00:00:00
abstract::We present functional imaging of the concentration of a photodynamic therapy (PDT)-related dye in scattering tissue phantoms based on spatially resolved measurements of optical properties through spectroscopic optical coherence tomography (OCT). Expressions for the OCT signal are developed, enabling estimation of dept...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1898242
更新日期:2005-03-01 00:00:00
abstract:SIGNIFICANCE:Although the clinical potential for Raman spectroscopy (RS) has been anticipated for decades, it has only recently been used in neurosurgery. Still, few devices have succeeded in making their way into the operating room. With recent technological advancements, however, vibrational sensing is poised to be a...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.25.5.050901
更新日期:2020-05-01 00:00:00
abstract::The transmembrane protein prestin is crucial to outer hair cell (OHC) electromotility and contributes to the sensitivity and frequency selectivity of mammalian hearing. The molecular mechanisms of electromotility remain unclear, but prestin is purported to function as both a voltage sensor and a molecular motor. Under...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2715202
更新日期:2007-03-01 00:00:00