Logarithmic intensity compression in fluorescence guided surgery applications.

abstract：SIGNIFICANCE:Visible light optical coherence tomography (OCT) is emerging for spectroscopic and ultrahigh resolution imaging, but challenges remain. Depth-dependent dispersion limits retinal image quality and current correction approaches are cumbersome. Inconsistent group refractive indices during image reconstruction...

journal_title：Journal of biomedical optics

authors： Zhang T,Kho AM,Srinivasan VJ

更新日期：2020-09-01 00:00:00

abstract：:Dispersion encoded full-range (DEFR) frequency-domain optical coherence tomography (FD-OCT) and its enhanced version, fast DEFR, utilize dispersion mismatch between sample and reference arm to eliminate the ambiguity in OCT signals caused by non-complex valued spectral measurement, thereby numerically doubling the usa...

journal_title：Journal of biomedical optics

authors： Wang L,Hofer B,Guggenheim JA,Povazay B

更新日期：2012-07-01 00:00:00

abstract：:Diffuse optical spectroscopy (DOS) provides a powerful tool for fast and noninvasive disease diagnosis. The ability to leverage DOS to accurately quantify tissue optical parameters hinges on the model used to estimate light-tissue interaction. We describe the accuracy of a lookup table (LUT)-based inverse model for me...

journal_title：Journal of biomedical optics

authors： Nichols BS,Rajaram N,Tunnell JW

更新日期：2012-05-01 00:00:00

abstract：:Optical quantification of large lesions imaged with diffuse optical tomography in reflection geometry is depth dependence due to the exponential decay of photon density waves. We introduce a depth-correction method that incorporates the target depth information provided by coregistered ultrasound. It is based on balan...

journal_title：Journal of biomedical optics

authors： Tavakoli B,Zhu Q

更新日期：2011-05-01 00:00:00

abstract：:Imaging large areas of tissue rapidly and with high resolution may enable rapid pathology at the bedside. The limited field of view of high-resolution microscopes requires the merging of multiple images that are taken sequentially to cover a large area. This merging or mosaicing of images requires long acquisition and...

journal_title：Journal of biomedical optics

authors： Abeytunge S,Li Y,Larson B,Toledo-Crow R,Rajadhyaksha M

更新日期：2011-05-01 00:00:00

abstract：:Waveguide evanescent field fluorescence (WEFF) microscopy is a new development that allows the imaging of contact regions between biological cells and their substratum, as well as imaging of ultrathin films such as monomolecular Langmuir-Blodgett (LB) films. Mixed-lipid monolayer systems mimicking lung surfactant were...

journal_title：Journal of biomedical optics

authors： Hassanzadeh A,Mittler S

更新日期：2011-04-01 00:00:00

abstract：:Image correlation spectroscopy (ICS) is a powerful technique for detecting arrangement of fluorophores in images. This tutorial gives background into the mathematical underpinnings of ICS, specifically image autocorrelation. The effects of various artifacts and image processing steps, including background subtraction,...

journal_title：Journal of biomedical optics

authors： Robertson C,George SC

更新日期：2012-08-01 00:00:00

abstract：:Laser-induced breakdown spectroscopy (LIBS) is applied to analyze human fingernails using nanosecond laser pulses. Measurements on 45 nail samples are carried out and 14 key species are identified. The elements detected with the present system are: Al, C, Ca, Fe, H, K, Mg, N, Na, O, Si, Sr, Ti as well as CN molecule. ...

journal_title：Journal of biomedical optics

authors： Hosseinimakarem Z,Tavassoli SH

更新日期：2011-05-01 00:00:00

abstract：SIGNIFICANCE:In breast-preserving tumor surgery, the inspection of the excised tissue boundaries for tumor residue is too slow to provide feedback during the surgery. The discovery of positive margins requires a new surgery which is difficult and associated with low success. If the re-excision could be done immediately...

journal_title：Journal of biomedical optics

authors： Beletkaia E,Dashtbozorg B,Jansen RG,Ruers TJM,Offerhaus HL

更新日期：2020-09-01 00:00:00

abstract：:The expensive fabrication of current opto-microfluidic sensors is a barrier to the successful adoption of these devices in point-of-care testing. This work reports a simple inexpensive opto-microfluidic device incorporating a poly(dimethylsiloxane)-glass hybrid microfluidic chip modified with gold nanoparticles and a ...

journal_title：Journal of biomedical optics

authors： Pires NM,Dong T

更新日期：2014-03-01 00:00:00

abstract：SIGNIFICANCE:The potential of fluorescence lifetime imaging microscopy (FLIM) is recently being recognized, especially in biological studies. However, FLIM does not directly measure the lifetimes, rather it records the fluorescence decay traces. The lifetimes and/or abundances have to be estimated from these traces dur...

journal_title：Journal of biomedical optics

authors： Guo S,Silge A,Bae H,Tolstik T,Meyer T,Matziolis G,Schmitt M,Popp J,Bocklitz T

更新日期：2021-01-01 00:00:00

abstract：:The use of reduced nicotinamide adenine dinucleotide (NADH) fluorescence to gain metabolic information on kidneys in response to an alteration in oxygen availability has previously been experimentally demonstrated, but signal quantification has not, to date, been addressed. In this work the relative contribution to ra...

journal_title：Journal of biomedical optics

authors： Raman RN,Pivetti CD,Rubenchik AM,Matthews DL,Troppmann C,Demos SG

更新日期：2009-03-01 00:00:00

abstract：:We present a quantitative analysis of dynamic diffuse optical measurements to obtain oxygen saturation of hemoglobin in volume oscillating compartments. We used a phasor representation of oscillatory hemodynamics at the heart rate and respiration frequency to separate the oscillations of tissue concentrations of oxyhe...

journal_title：Journal of biomedical optics

authors： Kainerstorfer JM,Sassaroli A,Fantini S

更新日期：2016-10-01 00:00:00

abstract：:Cerenkov light is created in clinical applications involving high-energy radiation such as in radiation therapy. There is considerable interest in using Cerenkov light as a means to perform in vivo dosimetry during radiation therapy; however, a better understanding of the light-to-dose relationship is needed. One such...

journal_title：Journal of biomedical optics

authors： Brost E,Watanabe Y

更新日期：2018-10-01 00:00:00

abstract：:Optically sectioned detection of fluorescence immunoassays using a confocal microscope enables the creation of both homo- and heterogeneous planar format assays. We report a set assays requiring optically sectioned detection using a model system and analysis procedures for separating signals of a surface layer from an...

journal_title：Journal of biomedical optics

authors： Ghafari H,Zhou Y,Ali S,Hanley QS

更新日期：2009-11-01 00:00:00

abstract：:Artificially created demineralized and remineralized carious lesions on the root and enamel of human teeth were examined by photothermal radiometry (PTR) and modulated luminescence (LUM). Fourteen extracted human teeth were used and a lesion was created on a 1 mmx4 mm rectangular window, spanning root to enamel, using...

journal_title：Journal of biomedical optics

authors： Jeon RJ,Hellen A,Matvienko A,Mandelis A,Abrams SH,Amaechi BT

更新日期：2008-05-01 00:00:00

abstract：:Doxorubicin is a commonly used chemotherapeutic employed to treat multiple human cancers, including numerous sarcomas and carcinomas. Furthermore, doxorubicin possesses strong fluorescent properties that make it an ideal reagent for modeling drug delivery by examining its distribution in cells and tissues. However, wh...

journal_title：Journal of biomedical optics

authors： Carlson M,Watson AL,Anderson L,Largaespada DA,Provenzano PP

更新日期：2017-11-01 00:00:00

abstract：:We propose a useful method to boost the imaging speed for spectral domain optical coherence tomography (SDOCT) by multiplying a number of high-speed spectrometers used in the system with selective precise control of data-recording and data-reading phases for spectral cameras employed in each spectrometer. To demonstra...

journal_title：Journal of biomedical optics

authors： An L,Guan G,Wang RK

更新日期：2011-06-01 00:00:00

abstract：:Given the several difficulties associated with histology, including difficulty in continuous monitoring, this study aimed to investigate the feasibility of optical imaging modalities—cross-polarization color (CPC) imaging, erythema index (EI) imaging, and laser speckle contrast (LSC) imaging—for continuous evaluation ...

journal_title：Journal of biomedical optics

authors： Son T,Yoon HJ,Lee S,Jang WS,Jung B,Kim WU

更新日期：2014-01-01 00:00:00

abstract：:Functional near-infrared spectroscopy (fNIRS), acquired simultaneously with electroencephalography (EEG), allows the investigation of hemodynamic brain responses to epileptic activity. Because the presumed epileptogenic focus is patient-specific, an appropriate source/detector (SD) montage has to be reconfigured for e...

journal_title：Journal of biomedical optics

authors： Machado A,Marcotte O,Lina JM,Kobayashi E,Grova C

更新日期：2014-02-01 00:00:00

abstract：:A microelectromechanical system (MEMS) mirror based endoscopic swept-source optical coherence tomography (SS-OCT) system that can perform three-dimensional (3-D) imaging at high speed is reported. The key component enabling 3-D endoscopic imaging is a two-axis MEMS scanning mirror which has a 0.8×0.8  mm2 mirror plate...

journal_title：Journal of biomedical optics

authors： Wang D,Fu L,Wang X,Gong Z,Samuelson S,Duan C,Jia H,Ma JS,Xie H

更新日期：2013-08-01 00:00:00

abstract：:Current diagnostic capabilities and limitations of fluorescence endomicroscopy in the cervix are assessed by qualitative and quantitative image analysis. Four cervical tissue types are investigated: normal columnar epithelium, normal and precancerous squamous epithelium, and stromal tissue. This study focuses on the p...

journal_title：Journal of biomedical optics

authors： Schlosser C,Bodenschatz N,Lam S,Lee M,McAlpine JN,Miller DM,Van Niekerk DJ,Follen M,Guillaud M,MacAulay CE,Lane PM

更新日期：2016-12-01 00:00:00

abstract：:The development of methods that allow microscale studies of complex biomaterials based on their molecular composition is of great interest to a wide range of research fields. We show that stimulated Raman scattering (SRS) microscopy is an excellent analytical tool to study distributions of different biomolecules in mu...

journal_title：Journal of biomedical optics

authors： Roeffaers MB,Zhang X,Freudiger CW,Saar BG,van Ruijven M,van Dalen G,Xiao C,Xie XS

更新日期：2010-11-01 00:00:00

abstract：:Optical technique based on diffuse reflectance measurement combined with indocyanine green (ICG) bolus tracking is extensively tested as a method for clinical assessment of brain perfusion in adults at the bedside. Methodology of multiwavelength and time-resolved detection of fluorescence light excited in the ICG is p...

journal_title：Journal of biomedical optics

authors： Gerega A,Milej D,Weigl W,Botwicz M,Zolek N,Kacprzak M,Wierzejski W,Toczylowska B,Mayzner-Zawadzka E,Maniewski R,Liebert A

更新日期：2012-08-01 00:00:00

abstract：:The optical activity of glucose molecules is important for diagnosing and monitoring blood glucose of diabetes. In order to accurately detect the diabetes at an early stage, there is an urgent need to develop innovative detection methods. By use of Mueller matrix decomposition (MMD), we have studied the optical activi...

journal_title：Journal of biomedical optics

authors： Sun P,Ma Y,Liu W,Yang Q,Jia Q

更新日期：2014-04-01 00:00:00

abstract：:Metal nanoparticles can be functionalized with biomolecules to selectively localize in precancerous tissues and can act as optical contrast enhancers for reflectance-based diagnosis of epithelial precancer. We carry out Monte Carlo (MC) simulations to analyze photon propagation through nanoparticle-labeled tissues and...

journal_title：Journal of biomedical optics

authors： Cihan C,Arifler D

更新日期：2011-08-01 00:00:00

abstract：:Intercellular heterogeneity is a key factor in a variety of core cellular processes including proliferation, stimulus response, carcinogenesis, and drug resistance. However, cell-to-cell variability studies at the single-cell level have been hampered by the lack of enabling experimental techniques. We present a measur...

journal_title：Journal of biomedical optics

authors： Kelbauskas L,Ashili SP,Houkal J,Smith D,Mohammadreza A,Lee KB,Forrester J,Kumar A,Anis YH,Paulson TG,Youngbull CA,Tian Y,Holl MR,Johnson RH,Meldrum DR

更新日期：2012-03-01 00:00:00

abstract：:We report an accurate optical differentiation technique between healthy and malaria-infected erythrocytes by quasi-simultaneous measurements of transmittance, reflectance, and scattering properties of unstained blood smears using a multispectral and multimode light-emitting diode microscope. We propose a technique for...

journal_title：Journal of biomedical optics

authors： Merdasa A,Brydegaard M,Svanberg S,Zoueu JT

更新日期：2013-03-01 00:00:00

abstract：:Pulse-modulated second harmonic imaging microscopes (PM-SHIMs) exhibit improved signal-to-noise ratio (SNR) over conventional SHIMs on sensitive imaging and quantification of weak collagen signals inside tissues. We quantify the spatial distribution of sparse collagen inside a xenograft model of human acute myeloid le...

journal_title：Journal of biomedical optics

authors： Raja AM,Xu S,Sun W,Zhou J,Tai DC,Chen CS,Rajapakse JC,So PT,Yu H

更新日期：2010-09-01 00:00:00

abstract：:In this study we examine the implications of excitation saturation on fluorescence recovery after photobleaching (FRAP) experiments. In particular we present both experimental and theoretical evidence that fluorescein, one of the most frequently used fluorophores in FRAP, does not always comply with the basic assumpti...

journal_title：Journal of biomedical optics

authors： Braeckmans K,Stubbe BG,Remaut K,Demeester J,De Smedt SC

更新日期：2006-07-01 00:00:00