Abstract:
:We present a method for tissue fluorescence quantification in situ using a handheld fiber optic probe that measures both the fluorescence and diffuse reflectance spectra. A simplified method to decouple the fluorescence spectrum from distorting effects of the tissue optical absorption and scattering is developed, with the objective of accurately quantifying the fluorescence in absolute units. The primary motivation is measurement of 5-aminolevulinic acid-induced protoporphyrin IX (ALA-PpIX) concentration in tissue during fluorescence-guided resection of malignant brain tumors. This technique is validated in phantoms and ex vivo mouse tissues, and tested in vivo in a rabbit brain tumor model using ALA-PpIX fluorescence contrast.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Kim A,Khurana M,Moriyama Y,Wilson BCdoi
10.1117/1.3523616subject
Has Abstractpub_date
2010-11-01 00:00:00pages
067006issue
6eissn
1083-3668issn
1560-2281journal_volume
15pub_type
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